Review
Chemistry, Multidisciplinary
Aurore Fraix, Cristina Parisi, Mimimorena Seggio, Salvatore Sortino
Summary: Nitric oxide (NO) is involved in various physiological and pathophysiological processes in the human body, and its controlled release through light activation is promising for therapeutic applications. Molecular constructs that can activate fluorescence while releasing NO offer the advantage of tracking the amount of released NO in real-time in a non-invasive manner. This article provides an overview of the advances in photoactivatable NO releasers with fluorescent reporting functionalities and their potential therapeutic applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Cristina Parisi, Francesca Moret, Aurore Fraix, Luca Menilli, Mariacristina Failla, Federica Sodano, Claudia Conte, Fabiana Quaglia, Elena Reddi, Salvatore Sortino
Summary: The biological activity of a molecular hybrid (DXNO-GR) combining doxorubicin (DOX) and nitric oxide (NO) has been studied against breast cancer cells. DXNO-GR shows higher cellular internalization compared to DOX and does not experience cell efflux in resistant cells. The cellular internalization of DXNO-GR is mediated by bovine serum albumin (BSA). DXNO-GR is not toxic in the dark but induces cell death following photoactivation with green light.
Article
Nanoscience & Nanotechnology
Chendong Ji, Xian Zheng, Shuolin Li, Chang Liu, Meizhen Yin
Summary: This study developed a dual light activatable perylenediimide-based photothermal agent for enhanced photothermal therapy. The agent releases nitric oxide and a photothermal molecule upon green light irradiation, achieving an improved photothermal effect and inhibiting cell protection autophagy. The release of the photothermal molecule can be monitored in real-time through NIR fluorescence.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Jing Sun, Yong Fan, Wei Ye, Limei Tian, Shichao Niu, Weihua Ming, Jie Zhao, Luquan Ren
Summary: A nitric oxide-assisted PDT nanocomposite membrane was developed for significantly enhanced bactericidal performances under a single near-infrared light irradiation. The generated nitric oxide exhibited a larger sterilization area compared to reactive oxygen species, demonstrating a vital complementary advantage in facilitating PDT antibacterial efficacy as proven in in vitro and in vivo tests. This type of nanocomposite membrane may have unique biomedical applications where both highly enhanced PDT antibacterial effects and deep penetration of NIR light are desired.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Maomao Song, Liping Li, Jiamin Liu, Yanting Gao, Mengwei Li, Liming Zhou, Bo Qin, Ajun Xiang, Xinghuai Sun, Wenpei Fan, Yuan Lei, Xiaoyuan Chen
Summary: In this study, a denitrifying hollow mesoporous organosilica nanoparticle (HMMN) was constructed to encapsulate S-nitroso-N-acetyl-d,l-penicillamine (SNAP) for controlled nitric oxide (NO) release and peroxynitrite removal. The designed SNAP@HMMN showed high corneal permeability and could lower intraocular pressure (IOP) in a precise and long-lasting manner while protecting the outflow tissues.
Article
Chemistry, Analytical
Fang Tang, Chang Gao, Jin-Yu Liu, Zhong-Lin Lu, Lan He, Ai-Xiang Ding
Summary: A new sensor, Lyso-DHP, was designed and synthesized for sensitive and selective detection of NO in lysosomes of living cells. This sensor exhibited fast response, high ?switch-on? ratio, low detection limit, and cytocompatibility. Localization studies confirmed its high specificity, precision, and brightness for tracing subcellular NO.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Pharmacology & Pharmacy
Francesca Laneri, Nadia Licciardello, Yota Suzuki, Adriana C. E. Graziano, Federica Sodano, Aurore Fraix, Salvatore Sortino
Summary: The chemotherapeutic Lenvatinib (LVB) and a nitric oxide (NO) photodonor based on a rhodamine antenna (RD-NO) are supramolecularly assembled within a polymeric host. The presence of LVB does not affect the photoinduced processes of RD-NO. The co-assembly of LVB and RD-NO in the same host is stable and does not lead to intermolecular photoinduced processes.
Article
Engineering, Biomedical
Kesi Wang, Linping Jiang, Liyan Qiu
Summary: In this study, a nano-vesicular system IA-PEP was developed to efficiently deliver photosensitizer PEGylated indocyanine green and NO donor L-Arg, achieving a ternary synergistic treatment combining NO therapy, photodynamic therapy, and mild-temperature photothermal therapy under 808 nm laser irradiation. The potent anti-tumor efficacy of IA-PEP with complete tumor elimination was verified in vivo in nude mice bearing xenograft MCF-7 tumors.
ACTA BIOMATERIALIA
(2022)
Article
Materials Science, Multidisciplinary
Kun Xu, Zhang Yuan, Yao Ding, Ye He, Ke Li, Chuanchuan Lin, Bailong Tao, Yulu Yang, Xuan Li, Peng Liu, Kaiyong Cai
Summary: This study developed a multi-mode synergetic therapy by immobilizing mesoporous dopamine nanoparticles loaded with a nitric oxide donor on the surface of titanium implants, aiming to promote antibacterial property and osseointegration.
APPLIED MATERIALS TODAY
(2021)
Article
Physiology
Agnes Keszler, Brian Lindemer, Grant Broeckel, Dorothee Weihrauch, Yan Gao, Nicole L. Lohr
Summary: Nitric oxide-dependent vasodilation facilitated by 670 nm red light has been studied in an ex vivo murine model and in vivo mouse experiments. The results show that red light can promote vasodilation and increase blood flow by releasing a vasoactive nitric oxide precursor. This treatment may offer a simple home-based therapy for individuals with impaired blood flow in the leg in the future.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Yuping Zhou, Wenbo Wu, Pengxiang Yang, Duo Mao, Bin Liu
Summary: The study presents a novel approach for the treatment of bacterial infection using nanoparticles that can achieve deep chemiluminescent imaging-guided photothermal therapy and nitric oxide gas therapy. The nanoparticles can precisely track infection-induced inflammation and actively eradicate bacteria through photothermal and nitric oxide therapy, leading to rapid recovery of infected tissues.
Article
Nanoscience & Nanotechnology
Baohong Sun, Ziqiu Ye, Ming Zhang, Qiuxian Song, Xiaohong Chu, Shurui Gao, Qicheng Zhang, Chen Jiang, Ninglin Zhou, Cheng Yao, Jian Shen
Summary: Chronic wound healing is hindered by bacterial infections and drug resistance. Antibacterial phototherapy combined with COFs offers a potential strategy for treating wound infections.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Shuting Zuo, Zhenyu Wang, Liping Zhao, Jing Wang
Summary: In this study, gold nanocages (GNCs) loaded with a hyperpyrexia-sensitive nitric oxide (NO) donor (thiolate cupferron) were constructed to integrate extrinsic radiosensitization, local photothermal therapy, and near-infrared-activated NO gas therapy. The resulting nanoplatform (GNCs@NO) showed excellent effects as a combination therapy for triple-negative breast cancer (TNBC), with high photothermal conversion efficiency and synergistic enhancement of anticancer efficacy through the production of reactive nitrogen species.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Aliya Tiemuer, Hui Yu, Chao Zhao, Wanlu Sun, Yuanyuan Zhang, Yiming Jiang, Yueqing Gu, Yi Liu
Summary: Gas therapy, particularly utilizing NO gas transmitter, has shown promise for effective cancer treatment. This study focused on addressing the drawbacks of current NO prodrugs, developing NIR light-triggered NO-releasing donors with superb spatiotemporal controllability for efficient NO delivery and real-time drug monitoring. The use of frequency upconversion luminescence (FUCL) readout for imaging provides high sensitivity and resolution, making it a potential breakthrough in deep-seated tumor therapy.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Cristina Parisi, Mariacristina Failla, Aurore Fraix, Luca Menilli, Francesca Moret, Elena Reddi, Barbara Rolando, Francesca Spyrakis, Loretta Lazzarato, Roberta Fruttero, Alberto Gasco, Salvatore Sortino
Summary: Researchers have developed a molecular hybrid that releases nitric oxide and superoxide anions, generating peroxynitrite upon red light activation. This hybrid shows good tolerance in various cancer cells and induces significant cell death under red light irradiation.