Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 21, Issue 13, Pages 5055-5060Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201406330
Keywords
carbon dots; cell imaging; fluorescence; functionalized c-dots; nucleoli
Categories
Funding
- CAPES
- FAPDF
- FINATEC
- CNPq
- INCT-Inami
- FACEPE [APT-0859-1.06/08]
- LabMIC(UFG)
- DPP-UnBand SMSdrug.net
- EPSRC [EP/I037229/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/I037229/1] Funding Source: researchfish
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Improved cellular selectivity for nucleoli staining was achieved by simple chemical modification of carbon dots (C-dots) synthesized from waste carbon sources such as cow manure (or from glucose). The C-dots were characterized and functionalized (amine-passivated) with ethylenediamine, affording amide bonds that resulted in bright green fluorescence. The new modified C-dots were successfully applied as selective live-cell fluorescence imaging probes with impressive subcellular selectivity and the ability to selectively stain nucleoli in breast cancer cell lineages (MCF-7). The C-dots were also tested in four other cellular models and showed the same cellular selection in live-cell imaging experiments.
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