Article
Food Science & Technology
Nieves Baenas, Sergio Bravo, Francisco Javier Garcia-Alonso, Jose Vicente Gil, Maria Jesus Periago
Summary: The industrial processing and cold storage of minimally processed tomato juice can lead to changes in volatile compounds responsible for fresh tomato flavor, mainly influenced by the activities of enzymes such as LOX and HPL. These changes affect the fresh and green aroma attributes in the refrigerated tomato juice, with a reduction in enzyme activities and alterations in volatile compounds content.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Chemistry, Applied
Michael Burns, Wenying Bi, Hui Kim, Manjinder S. Lall, Chao Li, Brian T. O'Neill
Summary: A biocatalytic cascade for producing tert-butyl ((2R,4R)-2-methyltetrahydro-2H-pyran-4-yl)carbamate 6 has been demonstrated at a multikilogram scale. The process involves resolving a racemic ketone by selectively reducing the undesired ketone and then catalyzing enantioselective transamination to form the desired amine. The product is recovered after Boc protection in the aqueous reaction.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Chemistry, Physical
Veronika Kazimirova, Viktoria Zezulova, Vladimir Krasnan, Vladimir Stefuca, Martin Rebros
Summary: Cis-3-hexenal and trans-2-hexenal are important chemicals in the food and perfume industries, produced through the plant lipoxygenase pathway involving enzymes like LOX and HPL. This study successfully expressed Pseudomonas aeruginosa LOX and Psidium guajava HPL in E. coli, enhancing activity and productivity through the addition of prosthetic groups and optimization of culture conditions.
Article
Biochemistry & Molecular Biology
Chiam Hashem, Julius Hochrinner, Moritz B. Buergler, Claudia Rinnofner, Harald Pichler, Margit Winkler
Summary: Green leaf volatiles (GLVs), with characteristic herbal and fruity scent, can be efficiently produced by introducing specific genes into yeast cells. The use of different induction systems has been applied to regulate the production of these volatiles.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Takuya Teshima, Risa Funai, Takehito Nakazawa, Junya Ito, Toshihiko Utsumi, Pattana Kakumyan, Hiromi Mukai, Toyoshi Yoshiga, Ryutaro Murakami, Kiyotaka Nakagawa, Yoichi Honda, Kenji Matsui
Summary: 1-Octen-3-ol is a volatile oxylipin commonly found in Basidiomycota and Ascomycota. The biosynthetic pathway of 1-octen-3-ol from linoleic acid has been characterized, but the enzymes involved are still unknown. This study identifies two genes, Ccdox1 and Ccdox2, in the mushroom Coprinopsis cinerea, and shows that CcDOX1 is responsible for the first step of 1-octen-3-ol synthesis. The study also suggests the involvement of another enzyme in the conversion of the intermediate compound. Furthermore, the study demonstrates the importance of 1-octen-3-ol in ecological communication in mushrooms.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Hyo Eun Jo, Kihwan Song, Jeong-Gu Kim, Choong Hwan Lee
Summary: The differences in VOC and primary metabolite composition were investigated among 20 representative cucumber lines. The Korean breeding line showed unique VOC composition in the fruit peel compared to the other groups, with lower levels of the main cucumber flavor components.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Anna Coenen, Valentin Gala Marti, Kira Mueller, Maria Sheremetiev, Lorenzo Finamore, Ulrich Schoerken
Summary: This study investigated the synthesis of 12-oxo-9(Z)-dodecenoic acid by cloning and expressing HPL from Carica papaya. Deleting the non-conserved N-terminal sequence improved the enzyme activity. The enzyme could efficiently catalyze the synthesis of 13S-HPODE and be used in a one-pot reaction.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Nur-Athirah Abd-Hamid, Muhammad Naeem-ul-hassan, Zamri Zainal, Ismanizan Ismail
Summary: Green leaf volatiles (GLVs) play important roles in plant defense, plant-plant interaction, and plant-insect interaction. This study found that overexpression of PmF-box1 gene inhibits the synthesis of GLVs, resulting in reduced volatile production. It also discovered that PmF-box1 interacts with SAMS1, influencing the expression of HPL pathway enzyme genes. Therefore, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions.
Article
Chemistry, Analytical
Marta Ostrihonova, Monika Antosova, Hana Dobiasova, Justina Cuchorova, Kvetoslava Vrankova, Milan Polakovic
Summary: Biocatalytic oxidation of a fatty acid preparation by enzymes released from a plant extract provided (2E,4E)-deca-2,4-dienal (2,4-DDAL), a valuable natural aroma. The highest binding capacity for 2,4-DDAL was around 30 mg/g among 17 hydrophobic adsorbents tested in batch equilibrium experiments. Ethanol was found to be the best desorbent for purifying 2,4-DDAL from the biocatalytic reaction mixture.
Article
Engineering, Environmental
Kevin Crouvisier Urion, Rebeca Garcia, Aline Boussard, Laure Degrand, Wafa Guiga
Summary: This study is the first to investigate the role of gas-liquid oxygen transfer on the determination of kinetic parameters of an oxidase and reactor sizing for pure hydroperoxides production. It was found that the addition of a small amount of ethanol was suitable for simple linoleic acid handling. By reprocessing dissolved oxygen kinetics, accurate reaction rates were determined, showing consistency with hydroperoxide production rates.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Chenming Huang, Junling Liu, Jiali Fang, Xian Jia, Zhendong Zheng, Song You, Bin Qin
Summary: This review discusses the advances in the research area of KRED-catalyzed asymmetric synthesis for biomanufacturing of chiral chemicals with at least two chiral centers through the kinetic resolution (KR) approach and the dynamic kinetic resolution (DKR) approach.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Svetlana S. Gorina, Tatiana M. Iljina, Lucia S. Mukhtarova, Yana Y. Toporkova, Alexander N. Grechkin
Summary: The genome of the neotropical fruit bat Sturnira hondurensis contains a gene encoding a plant-like protein, CYP74C44, which shares a high sequence identity with Populus trichocarpa. This study reveals that the recombinant CYP74C44 possesses the typical HPL activity, converting specific substrates into chain cleavage products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Ruth Chrisnasari, Marie Hennebelle, Jean -Paul Vincken, Willem J. H. van Berkel, Tom A. Ewing
Summary: This review focuses on the recent findings of the biochemical characteristics of bacterial LOXs and their molecular structures, exploring their potential applications in industrial processes and the approaches to improve their performance through mutagenesis.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Chemistry, Applied
Dongyan Li, He Dong, Xianwen Chen, Yangyang Li, Yiping Xu, Jing Wang, Jiali Fang, Chenming Huang, Wenhe Zhang, Xian Jia, Song You, Bin Qin
Summary: In this study, selective bio-reduction of Hajos-Parrish ketone (HPK, 1 a) and Wieland-Miescher ketone (WMK, 1 b) was achieved using Ene-reductase (ERED) from Bacillus subtilis (BsER) through protein engineering. Optically pure (R)-HPK ((R)-1 a) and (R)-WMK ((R)-1 b) were obtained with high enantiomeric excess (ee) values. Additionally, high diastereomeric ratio (dr) reduction products were also obtained. Biocatalytic cascade reduction routes involving BsER and ketoreductases (KREDs) were developed, leading to the stepwise reduction of (S)-HPK ((S)-1 a) and (S)-WMK ((S)-1 b).
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Daniel Maynard, Kamel Chibani, Sonja Schmidtpott, Thorsten Seidel, Jens Spross, Andrea Viehhauser, Karl-Josef Dietz
Summary: 13-lipoxygenases play a crucial role in catalyzing the dioxygenation of various polyunsaturated fatty acids in plants. This study focused on characterizing four annotated A. thaliana 13-LOX enzymes and their involvement in the synthesis of prostaglandin-like plant hormones. Results showed that 13-LOXs have specific functional and regulatory enzymatic features, and their activities are influenced by various physiologically relevant chemicals and molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)