Journal
ONCOLOGY REPORTS
Volume 36, Issue 6, Pages 3379-3386Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2016.5144
Keywords
miR-20a; human hepatocellular cancer; RUNX3
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Funding
- National Natural Science Foundation of China [81101744]
- Fundamental Research Funds for the Central Universities [xjj2014144]
- Funds for Technical Resources sharing Platform of Shaanxi Province, China [2015FWPT-14]
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Growing evidence indicates that some abnormally expressed microRNAs (miRNAs) influence tumorigenesis and progression. Previous studies reported that miR-20a is among the frequently altered miRNAs in human hepatocellular carcinoma (HCC), but its expression pattern and role in HCC remain controversial. In the present study, we demonstrated that miR-20a-5p exhibited aberrant expression in HCC tissues compared with paired non-tumor tissues: 52% of the tumor samples showed a greater increase. Overexpression of miR-20a contributed to HCC cell proliferation and migration in vitro, and treatment with anti-miR20a-5p caused the opposite effects. Further studies revealed RUNX3, an important tumor-suppressor, as a direct target of miR-20a-5p. We observed that the level of RUNX3 was sharply reduced in both mRNA and protein in HCC tissues compared with paired non tumor tissues. Collectively, our results support the viewpoint that miR-20-5p has an oncogenic property, miR-20a overexpression contributed to HCC cell proliferation and migration through reducing the translation of RUNX3. The data provide a new mechanism of miR-20a regulating RUNX3 in HCC.
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