Article
Multidisciplinary Sciences
Mohammed Rizwan Babu Sait, Hendrik Koliwer-Brandl, Jessica A. Stewart, Benjamin M. Swarts, Marc Jacobsen, Thomas R. Ioerger, Rainer Kalscheuer
Summary: The disaccharide trehalose is essential for the viability of Mycobacterium tuberculosis, which synthesizes trehalose de novo but can also use exogenous trehalose. The transport of trehalose and its analogues across the mycomembrane of M. tuberculosis is exclusively mediated by the PPE51 membrane protein. Mutants lacking the PPE51 gene are unable to grow on trehalose as the sole carbon source.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Cecilia B. Di Capua, Juan M. Belardinelli, Hugo A. Carignano, Maria V. Buchieri, Cristian A. Suarez, Hector R. Morbidoni
Summary: Mycolic acids, unique long-chain fatty acids produced by Mycobacterium, play vital roles in pathogenesis and are potential drug targets. This study characterized a mutant of Mycobacterium smegmatis deficient in alpha'-mycolic acids and identified the enzyme responsible for their synthesis. This finding sheds light on the growth and development of Mycobacterium abscessus and offers insights for drug development.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Medicinal
Hongyi Zhao, Yongxin Gao, Wei Li, Li Sheng, Keli Cui, Bin Wang, Lei Fu, Meng Gao, Ziyun Lin, Xiaowen Zou, Mary Jackson, Haihong Huang, Yu Lu, Dongfeng Zhang
Summary: In this study, pyrrole-2-carboxamides were designed and synthesized as potential anti-TB agents based on the crystal structure of MmpL3 and a pharmacophore model. Structure-activity relationship studies revealed the importance of specific substituents on the pyrrole ring and carboxamide moiety in enhancing the anti-TB activity. One compound showed excellent activity against drug-resistant tuberculosis and exhibited promising pharmacological properties. The target of these pyrrole-2-carboxamides was identified through functional assays. This study provides new MmpL3 inhibitors that may serve as effective treatments for tuberculosis.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Stephanie A. Valenzuela, Hannah S. N. Crory, Chao-Yi Yao, James R. Howard, Gabriel Saucedo, A. Prasanna de Silva, Eric V. Anslyn
Summary: A colorimetric indicator displacement assay was developed for high-throughput experimentation to determine the percentage of cis and trans alkenes. The method showed high accuracy in determining the percentage of alkene configurations and can be optimized for human eye response by tuning the color.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Organic
Jun-Han Ma, Philippe Jubault, Samuel Couve-Bonnaire, Li-Wen Xu, Jean-Philippe Bouillon
Summary: We report a rhodium-catalyzed method for the regioselective hydrosilylation of trifluoromethylalkenes with substituted silanes, giving α-trifluoromethyl-β-silanes in good to excellent yields. The hydrogenation products were obtained by treating the key intermediate with methanol as a protic solvent. Both transformations exhibited a broad functional tolerance and were expected to facilitate the synthesis of complex α-trifluoromethyl compounds.
Article
Multidisciplinary Sciences
Charlotte Cooper, Eliza J. R. Peterson, Rebeca Bailo, Min Pan, Albel Singh, Patrick Moynihan, Makoto Nakaya, Nagatoshi Fujiwara, Nitin Baliga, Apoorva Bhatt
Summary: This study reveals that the mycolic acid desaturase regulator (MadR) controls mycolic acid desaturation and biosynthesis in response to cell envelope stress. This unique regulation mechanism is distinct to other regulators and MadR acts as a key regulatory checkpoint during infection to coordinate mycolic acid remodeling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Anuradha Kumar, Somsundaram Chettiar, Brian S. Brown, Julie Early, Juliane Ollinger, Megan Files, Mai A. Bailey, Aaron Korkegian, Devon Dennison, Matthew McNeil, James Metz, Augustine Osuma, Michael Curtin, Aaron Kunzer, Gail Freiberg, Milan Bruncko, Dale Kempf, Tanya Parish
Summary: A high-throughput phenotypic screening of a diverse chemical library identified 24 chemotypes with anti-tubercular activity. Further exploration revealed that MmpL3 may serve as the target or mechanism of resistance for these compounds.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Vaishnav Bhaskar, Sunil Kumar, Aathira Sujathan Nair, S. Gokul, Prayaga Rajappan Krishnendu, Sonu Benny, C. T. Amrutha, Deepthi S. Manisha, Vaishnavi Bhaskar, Subin Mary Zachariah, T. P. Aneesh, Mohamed A. Abdelgawad, Mohammed M. Ghoneim, Leena K. Pappachen, Orazio Nicolotti, Bijo Mathew
Summary: In this study, an atom-based 3D QSAR model was developed to screen potential candidates for the treatment of drug-resistant tuberculosis. Through molecular docking, ADME profiling, and molecular dynamic simulations, the lead molecule ZINC11536150 was found to exhibit strong antibacterial activity and stability.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Physical
Sumin Lee, Tomislav Rovis
Summary: This study presents a Rh(III)-catalyzed three-component carboamination of alkenes, allowing for the efficient and regioselective synthesis of valuable amine products, including alpha-amino acid derivatives, without the need for directing functionalities.
Article
Chemistry, Multidisciplinary
Omrane Kadri, Abdulrahman Alfuraih
Summary: This paper investigates the application of tissue mimicking material (TMM) in the field of medical radiology. By calculating the buildup factor (BUF) and simulating the percentage depth dose (PDD), several TMMs that accurately mimic different human tissues were identified. This study is of importance for physicians and researchers in the field of dosimetry and radiological diagnosis.
APPLIED SCIENCES-BASEL
(2022)
Article
Acoustics
Hadiseh Imanian, Mina Noori, Amin Abbasiyan
Summary: This article presents a gas sensor with a single detection unit that can detect multiple gases and their different concentrations. The proposed sensor is simple, scalable to operate at any desired frequency range, and provides a multi-peak detection mechanism.
Article
Biology
Amelia C. McKitterick, Thomas G. Bernhardt
Summary: Bacteria of the order Corynebacteriales, known for their complex cell envelope structure, serve as potent barriers against antibiotic entry into cells. Studying the mechanisms underlying this structure holds promise for the development of new antibiotics. Phage interactions with host factors during infection reveal crucial genes and pathways involved in cell wall synthesis.
Article
Microbiology
Lynn G. Dover, Amy R. Thompson, Iain C. Sutcliffe, Vartul Sangal
Summary: The Corynebacterium genus includes diverse species with different pathogenic potentials, many of which lack the capability for de novo fatty acid biosynthesis. Interestingly, although a mycolic acid-based mycomembrane is widely considered a target for interventions, it is not essential in Corynebacterium species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Xue Lin, Wei Jia, Gangning Feng, Yajing Su, Yuting Kang, Chen Zhang, Wenmiao Liu, Zhidong Lu, Di Xue
Summary: This study investigates the role of a panned heptapeptide in Mycobacterium-derived mycolic acid-induced cell injury. The heptapeptide was found to inhibit apoptosis, increase autophagy, and reduce inflammation and lung damage caused by mycolic acid. It has the potential to be developed as a novel therapeutic treatment for tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Yutaro Kumagai, Takashi Hirasawa, Masaaki Wachi
Summary: The study investigated the intracellular localization of wild-type and mutant LtsA proteins fused with GFP, revealing that the C-terminal domain is required for peripheral localization. Furthermore, differential staining of cell wall structures showed that the ltsA mutant cells had impaired formation of the mycolic acid-containing layer at the cell division planes, leading to increased susceptibility to lysozyme.
Article
Immunology
Hermann Giresse Tima, Juma'a Raheem Al Dulayymi, Olivier Denis, Pauline Lehebel, Klarah Sherzad Baols, Mohsin Omar Mohammed, Laurent L'Homme, Mohaned Mohammed Sahb, Georges Potemberg, Sylvie Legrand, Roland Lang, Rudi Beyaert, Jacques Piette, Mark Stephen Baird, Kris Huygen, Marta Romano
JOURNAL OF INNATE IMMUNITY
(2017)
Article
Biochemistry & Molecular Biology
Ilke Vermeulen, Mark Baird, Juma Al-Dulayymi, Muriel Smet, Jan Verschoor, Johan Grooten
JOURNAL OF LIPID RESEARCH
(2017)
Article
Multidisciplinary Sciences
Alison Jones, Mark Pitts, Juma'a R. Al Dulayymi, James Gibbons, Andrew Ramsay, Delia Goletti, Christopher D. Gwenin, Mark S. Baird
Article
Multidisciplinary Sciences
Heena Ranchod, Fortunate Ndlandla, Yolandy Lemmer, Mervyn Beukes, Johann Niebuhr, Juma Al-Dulayymi, Susan Wemmer, Jeanni Fehrsen, Mark Baird, Jan Verschoor
Article
Biochemistry & Molecular Biology
Omar T. Ali, Mohsin O. Mohammed, Paul J. Gates, Mark S. Baird, Jumaa R. Al Dulayymi
CHEMISTRY AND PHYSICS OF LIPIDS
(2019)
Article
Biochemistry & Molecular Biology
Omar T. Ali, Mohsin O. Mohammed, Juma'a R. Al Dulayymi, Mark S. Baird
Article
Biochemistry & Molecular Biology
Alison Jones, Oona Y-C Lee, David E. Minnikin, Mark S. Baird, Juma'a R. Al Dulayymi
CHEMISTRY AND PHYSICS OF LIPIDS
(2020)
Article
Biochemistry & Molecular Biology
Zamzam S. Alhuwaymil, Intisar Q. M. Al-araj, Ahmad R. Al Dulayymi, Alison Jones, Paul J. Gates, Pedro L. Valero-Guillen, Mark S. Baird, Juma'a R. Al Dulayymi
CHEMISTRY AND PHYSICS OF LIPIDS
(2020)
Article
Parasitology
Anand Chakroborty, Deiniol Pritchard, Marc E. Bouillon, Anna Cervi, Alan Cookson, Charlotte Wild, Caroline Fenn, Joseph Payne, Peter Holdsworth, Colin Capner, Jenna O'Neill, Gilda Padalino, Josephine Forde-Thomas, Sandeep Gupta, Brendan G. Smith, Maggie Fisher, Martina Lahmann, Mark S. Baird, Karl F. Hoffmann
Summary: Control of liver fluke infections is a significant challenge in the livestock sector due to drug resistant parasites. This study evaluated the anthelmintic activities of chemically modified abietic acid analogues against F. hepatica and found moderate activity in one analogue, but it also showed toxicity to mammalian cells. Therefore, further medicinal chemistry improvements are needed to consider these analogues as potential anthelmintics against liver fluke pathogens.
VETERINARY PARASITOLOGY
(2022)
Editorial Material
Respiratory System
Julia Bashford, William Flowers, Charles Haworth, Judy Ryan, Anna Cervi, J. R. Al Dulayymi, Paul S. Mason, Ashley Plank, Mark Baird, Andres Floto
Summary: Diagnosing non-tuberculous mycobacteria (NTM) in people with cystic fibrosis is challenging. Current methods rely on sputum culture, which is resource demanding and may be compromised by various factors. This study tested the effectiveness of synthetic mycolic acids and glycopeptidolipid (GPL)-core antigen in distinguishing patients with Mycobacterium abscessus infection. The results showed promising diagnostic accuracy, suggesting potential use in clinical practice.
Article
Biochemistry & Molecular Biology
Alison Jones, Carys A. Davies, Samuel Fitch, Juma'a R. Al Dulayymi, Christopher D. Gwenin, James Gibbons, A. Prysor Williams, Mark S. Baird
Summary: The study conducted ELISA tests on serum samples from different populations to detect antibody responses to mycobacterial esters, finding that individuals exposed to mycobacteria, such as farmers, showed significantly higher antibody responses, especially older farmers. This suggests that mycobacterial exposure may lead to differences in antibody responses among different population groups.
RSC MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Orlagh Anderson, Joseph Beckett, Carla C. Briggs, Liam A. Natrass, Charles F. Cranston, Elizabeth J. Wilkinson, Jack H. Owen, Rhodri Mir Williams, Angelos Loukaidis, Marc E. Bouillon, Deiniol Pritchard, Martina Lahmann, Mark S. Baird, Paul W. Denny
RSC MEDICINAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Omar T. Ali, Mohaned M. Sahb, Juma'a R. Al Dulayymi, Mark S. Baird
CARBOHYDRATE RESEARCH
(2017)
Article
Chemistry, Organic
Chioma Don Lawson, Max Maza-Iglesias, Muthana M. Sirhan, Juma'a R. Al Dulayymi, Mark S. Baird
Article
Biochemistry & Molecular Biology
Mohsin O. Mohammed, Juma'a R. Al Dulayymi, Mark S. Baird
CARBOHYDRATE RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Raja Narayanasamy, Dandamudi Usharani, Ram Rajasekharan
Summary: This study investigated the role of ABHD16B in lipid metabolism. The overexpression of ABHD16B was found to decrease cellular triacylglycerol levels and increase phospholipid synthesis in yeast cells. Additionally, ABHD16B overexpression led to a reduction in lipid droplets and significant modifications in fatty acid composition. These findings highlight the importance of ABHD16B in lipid homeostasis and provide insights into its regulatory function in cellular lipid metabolism.
CHEMISTRY AND PHYSICS OF LIPIDS
(2024)
