Small secreted peptides (SSPs) in tomato and their potential roles in drought stress response

Tomato (Solanum lycopersicum) is one of the most important vegetable crops in the world and abiotic stresses often cause serious problems in tomato production. It is thus important to identify new regulators in stress response and to devise new approaches to promote stress tolerance in tomato. Previous studies have shown that small secreted peptides (SSPs) are important signal molecules regulating plant growth and stress response by mediating intercellular communication. However, little is known about tomato SSPs, especially their roles in responding to abiotic stresses. Here we report the identification of 1,050 putative SSPs in the tomato genome, 557 of which were classified into 38 known SSP families based on their conserved domains. GO and transcriptome analyses revealed that a large proportion of SlSSPs might be involved in abiotic stress response. Further analysis indicated that stress response related cis-elements were present on the SlCEP promotors and a number of SlCEPs were significantly upregulated by drought treatments. Among the drought-inducible SlCEPs, SlCEP10 and SlCEP11b were selected for further analysis via exogenous application of synthetic peptides. The results showed that treatments with both SlCEP10 and SlCEP11b peptides enhanced tomato drought stress tolerance, indicating the potential roles of SlSSPs in abiotic stress response.

Automated summary

This study identified 1,050 putative SSPs in the tomato genome and showed that a large proportion of SlSSPs might be involved in abiotic stress response. The presence of stress response related cis-elements on the SlCEP promotors and significant upregulation of some SlCEPs by drought treatments were also observed. Exogenous application of synthetic peptides SlCEP10 and SlCEP11b enhanced tomato drought stress tolerance, indicating the potential roles of SlSSPs in abiotic stress response.