Article
Biochemistry & Molecular Biology
Lucia Piro, Sabrina Flutsch, Diana Santelia
Summary: Research showed that starch in stomatal guard cells is mainly synthesized using carbon precursors from imported sugars. The cytosolic sucrose synthase (SUS) was found to play a role in this process. Among the Arabidopsis SUS gene family, SUS3 was highly expressed in guard cells. The sus3 mutant displayed a similar guard cell starch content to the wild type initially, but later began to accumulate starch at a much higher rate, suggesting that SUS3 regulates carbon fluxes for starch synthesis during the second half of the day.
PLANT SIGNALING & BEHAVIOR
(2023)
Review
Cell Biology
P. Lemonnier, T. Lawson
Summary: Stomatal conductance plays a crucial role in determining CO2 uptake and water loss in plants, affecting overall water status and productivity. However, the signals coordinating mesophyll demands for CO2, the role of chloroplasts in stomatal function, and other GC metabolic processes in stomatal function remain poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Article
Plant Sciences
Young-Sun Riu, Hyun-Geun Song, Hwi-Su Kim, Sam-Geun Kong
Summary: This study used different promoters to alter the response characteristics and growth phenotypes of phototropins in transgenic plants, as well as improve leaf transpiration.
Article
Biochemistry & Molecular Biology
Chao Han, Wenbo Hua, Jinge Li, Yan Qiao, Lianmei Yao, Wei Hao, Ruizi Li, Min Fan, Geert De Jaeger, Wenqiang Yang, Ming-Yi Bai
Summary: TOR promotes guard cell starch degradation and induces stomatal opening by regulating the expression and degradation of BAM1. This process is crucial for the growth and photosynthetic efficiency of plants. Sugar and TOR signaling pathways play important roles in regulating BAM1 activity, allowing carbon availability to control guard cell starch metabolism and stomatal movement.
Review
Plant Sciences
Michael R. Blatt, Mareike Jezek, Virgilio L. Lew, Adrian Hills
Summary: Stomatal pores enable gaseous exchange between leaf air spaces and the atmosphere, opening for CO2 entry during photosynthesis and closing to minimize water loss. Modeling the response of stomata to the environment has been an area of interest to understand its implications for plants and ecosystems. While models focusing on stomatal conductance make sense, it is important to connect them with the mechanics of guard cells that regulate pore size in order to comprehend the decisions made by stomata and their effects on the plant and global environment.
TRENDS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Kosuke Moriwaki, Shuichi Yanagisawa, Koh Iba, Juntaro Negi
Summary: In this study, two crucial cis-acting elements, 5'-CACGAGA-3 'and 5'-CACATGTTTCCC-3', were found to be essential for the guard cell-specific expression of SCAP1 and HT1. The findings reveal a novel transcriptional regulatory mechanism that synchronously promotes the expression of multiple genes required for stomatal maturation and function.
Article
Plant Sciences
Hui-Chen Wu, Shih-Yu Yu, Yin-Da Wang, Tsung-Luo Jinn
Summary: A study identified and characterized an Arabidopsis type-II PME, PME53, which plays an important role in stomatal development and movement, as well as heat tolerance. The expression of PME53 is regulated by stomatal differentiation factors and abscisic acid, affecting stomatal number and ABA-induced closure. PME53 may be involved in the reconstitution of Ca2+-pectate for plant thermotolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Chuanlei Xiao, Huimin Guo, Jing Tang, Jiaying Li, Xuan Yao, Honghong Hu
Summary: The study systematically investigated the expression patterns of 19 putative Guard-cell-enriched GDSL Lipases (GGLs) in Arabidopsis, revealing diverse expression patterns in guard cells. Most GGLs were localized in the endoplasmic reticulum, and some in lipid droplets and nucleus. Further analysis showed the correlation of expression pattern and biological function, as well as the functional redundancy of GGLs in plant development and environmental adaptations. Phenotypic identification of ggl mutants indicated unique and redundant roles of GGLs in stomatal dynamics, density, morphology, and plant water relation, providing insights for controlling stomatal dynamics and development, plant growth, and adaptation to the environment.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Kazuma Sakoda, Wataru Yamori, Michael Groszmann, John R. Evans
Summary: In fluctuating light conditions, both stomatal conductance and Vcmax/J play a significant role in limiting photosynthesis during induction, suggesting that these factors are crucial for understanding the dynamics of leaf photosynthesis.
Article
Plant Sciences
Francisco Bruno S. Freire, Ricardo L. G. Bastos, Raissa S. C. Bret, Silvio A. Candido-Sobrinho, David B. Medeiros, Werner C. Antunes, Alisdair R. Fernie, Danilo M. Daloso
Summary: Regulation of stomatal speed can be achieved by manipulating the expression of guard cell sucrose synthase 2 (NtSUS2), leading to slower light-induced stomatal opening, lower steady-state stomatal conductance, and reduced whole plant transpiration. Additionally, plants with reduced NtSUS2 expression show higher water use efficiency under short water deficit periods. This suggests a complex modulation of the guard cell metabolic network in regulating stomatal movement and speediness.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Lisa David, Jianing Kang, Daniel Dufresne, Dan Zhu, Sixue Chen
Summary: Systemic Acquired Resistance (SAR) enhances plant immunity against pathogens, with guard cell response playing a key role. Prior exposure to pathogenic bacteria alters stomatal response in plants, leading to reduced bacterial levels in leaves. SAR activation of guard cells involves specific molecular components that include reactive oxygen species and fatty acid signaling, potentially influencing stomatal immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Chuwei Lin, Dawei Dai, Guimei Zhao, Sarah Joe, Sixue Chen
Summary: In this study, the role of MPK4 in stomatal immune response was explored through overexpression in Arabidopsis. The MPK4-associated protein complex in guard cells was characterized, and four new interacting proteins were identified. These findings shed light on the functions of MPK4 in guard cells and provide new targets for further research.
JOURNAL OF PROTEOMICS
(2023)
Review
Plant Sciences
Rosario Pantaleno, Denise Scuffi, Carlos Garcia-Mata
Summary: Hydrogen sulphide (H2S), an endogenously produced gasotransmitter, plays a crucial role in various plant processes, including the regulation of stomatal movement. Guard cells control stomatal pore size by transducing endogenous and environmental signals through a complex signaling network, with a core linking most pathways. Evidence suggests that H2S may act as a regulator of the core signaling pathway in guard cell networks.
Article
Biochemistry & Molecular Biology
Qingting Liu, Xiaoping Li, Joerg Fettke
Summary: By using laser confocal scanning microscopy, researchers found that in Arabidopsis mutants with altered starch metabolism, guard cells contained more but smaller plastidial starch granules compared to mesophyll cells. The morphological properties of the starch granules were indiscernible in both types of leaf cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jorge Gago, Miquel Nadal, Maria Jose Clemente-Moreno, Carlos Maria Figueroa, David Barbosa Medeiros, Neus Cubo-Ribas, Lohengrin Alexis Cavieres, Javier Gulias, Alisdair Robert Fernie, Jaume Flexas, Leon Aloys Bravo
Summary: Deschampsia antarctica is a native vascular plant in Antarctica, and the availability of nutrients in the soil affects its photosynthetic and stress tolerance mechanisms. Plants growing on low-nutrient availability soils have more limiting photosynthetic and stress tolerance performances, while plants in nutrient-rich soils show vigorous growth without appreciable levels of stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Paula da Fonseca-Pereira, Paulo V. L. Souza, Liang-Yu Hou, Saskia Schwab, Peter Geigenberger, Adriano Nunes-Nesi, Stefan Timm, Alisdair R. Fernie, Ina Thormaehlen, Wagner L. Araujo, Danilo M. Daloso
PLANT CELL AND ENVIRONMENT
(2020)
Review
Plant Sciences
Paula da Fonseca-Pereira, Paulo V. L. Souza, Alisdair R. Fernie, Stefan Timm, Danilo M. Daloso, Wagner L. Araujo
Summary: Thioredoxins play a crucial role in the redox regulation of plant metabolism, activating photosynthesis and adjusting mitochondrial photorespiratory metabolism. Mitochondrial TRX o1 has been identified as a regulator for alternative oxidase and enzymes associated with the tricarboxylic acid cycle, as well as being involved in photorespiration and branched chain amino acid degradation.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Francisco Bruno S. Freire, Ricardo L. G. Bastos, Raissa S. C. Bret, Silvio A. Candido-Sobrinho, David B. Medeiros, Werner C. Antunes, Alisdair R. Fernie, Danilo M. Daloso
Summary: Regulation of stomatal speed can be achieved by manipulating the expression of guard cell sucrose synthase 2 (NtSUS2), leading to slower light-induced stomatal opening, lower steady-state stomatal conductance, and reduced whole plant transpiration. Additionally, plants with reduced NtSUS2 expression show higher water use efficiency under short water deficit periods. This suggests a complex modulation of the guard cell metabolic network in regulating stomatal movement and speediness.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Youjun Zhang, Jonas Giese, Sandra M. Kerbler, Beata Siemiatkowska, Leonardo Perez de Souza, Jessica Alpers, David Barbosa Medeiros, Dirk K. Hincha, Danilo M. Daloso, Mark Stitt, Iris Finkemeier, Alisdair R. Fernie
Summary: This study identified the roles of two mitochondrial phosphatases, Sal2 and PP2c63, along with pyruvate dehydrogenase kinase (PDK), in regulating the tricarboxylic acid (TCA) cycle. PDK specifically regulates the pyruvate dehydrogenase complex (PDC), while PP2c63 nonspecifically regulates PDC. The study also showed that these phosphatases affect flux through the TCA cycle and altered metabolism compromises plant growth in mutant lines.
Article
Plant Sciences
E. F. Capelari, L. dos Anjos, N. F. Rodrigues, R. M. de J. Sousa, J. A. G. Silvera, R. Margis
Summary: Water stress severely impacts plant performance and crop yield, but the cashew tree has shown high adaptability and resilience to acute water deficit without activating senescence pathways. Integrative studies combining physiological and transcriptomic data revealed the main adaptive mechanisms of cashew to drought, particularly in carbon metabolism, photosynthesis, and chloroplast homeostasis.
Article
Plant Sciences
Valeria F. Lima, Alexander Erban, Andre G. Daubermann, Francisco Bruno S. Freire, Nicole P. Porto, Silvio A. Candido-Sobrinho, David B. Medeiros, Markus Schwarzlaender, Alisdair R. Fernie, Leticia dos Anjos, Joachim Kopka, Danilo M. Daloso
Summary: C-13 metabolic flux analysis has advanced our understanding of plant metabolic regulation, but detailed in vivo flux mapping remains challenging. NMR-based flux investigations offer high accuracy for small networks, while mass spectrometry approaches have broader potential but are limited by lack of atomic level position information.
Correction
Plant Sciences
Paula da Fonseca-Pereira, Paulo V. L. Souza, Alisdair R. Fernie, Stefan Timm, Danilo M. Daloso, Wagner L. Araujo
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Silvio Candido-Sobrinho, Valeria Lima, Francisco Freire, Leonardo de Souza, Jorge Gago, Alisdair R. Fernie, Danilo M. Daloso
Summary: Recent research suggests that metabolism-mediated stomatal closure mechanisms play a crucial role in regulating the differences in stomatal speediness between ferns and angiosperms. Ferns showed a lack of response to ABA and slower stomatal responses to sucrose and mannitol compared to angiosperms. Metabolomics analysis revealed that ferns accumulate more secondary metabolites than primary metabolites throughout the diel course, presenting a potential mechanism for the slower stomatal closure and differences in response to exogenous substances between ferns and angiosperms.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Priscila A. Auler, Francisco Bruno S. Freire, Valeria F. Lima, Danilo M. Daloso
Summary: The ability of plants to perceive and respond to environmental signals through guard cells is crucial for their survival and stress tolerance. Recent studies have shown that guard cells play a key role in stress memory and water use efficiency. Investigating the complexity of guard cell structure and signaling networks can provide insights into plant adaptation to stress and improve drought tolerance.
THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Livia L. Cardoso, Francisco Bruno S. Freire, Danilo M. Daloso
Summary: Plant stress acclimation depends on metabolic changes. This study used a multi-species/stress condition meta-analysis to investigate how plant metabolic network is altered under stress conditions. It was found that amino acid metabolism plays a crucial role in plant stress acclimation.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Plant Sciences
Nicole P. Porto, Raissa S. C. Bret, Paulo V. L. Souza, Silvio A. Candido-Sobrinho, David B. Medeiros, Alisdair R. Fernie, Danilo M. Daloso
Summary: The study found that TRX o1 restricts the carbon flux from glycolysis and TCAC to glutamine under light conditions. In contrast to the wild type, mutants exhibited significant changes in TCAC metabolite content and 13C enrichment under both dark and light conditions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Danilo de Menezes Daloso, Eva Gomes Morais, Karen Fernanda Oliveira e Silva, Thomas Christopher Rhys Williams
Summary: Every plant organ contains different cell types with specialized functions. Investigating cell-type-specific metabolism is challenging due to the complexity of different cells within tissues. However, techniques like cell isolation, microscopy, and modeling have provided insight into cell-type-specific metabolism.
Article
Plant Sciences
Valeria F. Lima, Francisco Bruno S. Freire, Silvio A. Candido-Sobrinho, Nicole P. Porto, David B. Medeiros, Alexander Erban, Joachim Kopka, Markus Schwaerzlander, Alisdair R. Fernie, Danilo M. Daloso
Summary: Evidence suggests that guard cells have higher rate of phosphoenolpyruvate carboxylase (PEPc)-mediated dark CO2 assimilation than mesophyll cells. However, it is unknown which metabolic pathways are activated following dark CO2 assimilation in guard cells. Furthermore, it remains unclear how the metabolic fluxes throughout the tricarboxylic acid (TCA) cycle and associated pathways are regulated in illuminated guard cells.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Eduardo Rodrigues Pessoa, Fabio Roger Vasconcelos, Stelamaris de Oliveira Paula-Marinho, Danilo de Menezes Daloso, Denise Damasceno Guerreiro, Jorge Andre Matias Martins, Eneas Gomes-Filho, Arlindo Alencar Moura
Summary: This study evaluated the seminal plasma metabolome of Bos indicus Guzera bulls with good and poor sperm freezability. Sixty-two metabolites were identified in the seminal plasma, with fatty acids and conjugates and organic compounds being the predominant metabolites. Multivariate analysis showed a distinct separation of seminal plasma metabolomes between bulls with contrasting sperm freezability. Specific metabolites, such as propanoic acid, d-ribose, and glycine, were found to be associated with good sperm freezability, while heptadecanoic acid and undecanoic acid were predominant in bulls with poor sperm freezability. This study provides important information about the seminal plasma metabolome of tropically adapted bulls and its association with sperm freezability.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
