Journal
NEUROSCIENCE LETTERS
Volume 633, Issue -, Pages 227-234Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2016.09.032
Keywords
Mesenchymal stem cell; Complement component 3; Neuroprotection; Cerebral ischemia; Oxygen glucose deprivation
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Funding
- Medical Research Center Program through National Research Foundation of Korea (NRF) - Korea government (MSIP) [2011-0030074]
- Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [NRF-2015R1A2A1A15051703]
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Bone marrow-derived mesenchymal stem cells (MSCs) are used in stroke treatment despite the poor understanding of its mode of action. The immune suppressive and anti-inflammatory properties of MSCs possibly play important roles in regulating neuroinflammation after stroke. We investigated whether MSCs reduce the inflammatory complement component 3 (C3) levels, thus, providing neuroprotection during stroke. Mice were subjected to transient focal cerebral ischemia (tFCI), after which MSCs were intravenously injected. The infarct volume of the brain was reduced in MSC-injected tFCI mice, and C3 expression was significantly reduced in both the brain and the blood. Additionally, the profiles of other inflammatory mediators demonstrated neuroprotective changes in the MSCs-treated group. In order to analyze the effect of MSCs on neurons during cerebral ischemia, primary cortical neurons were co cultured with MSCs under oxygen-glucose deprivation (OGD). Primary neurons co -cultured with MSCs exhibited reduced levels of C3 expression and increased protection against OGD, indicating that treatment with MSCs reduces excessive C3 expression and rescues ischemia-induced neuronal damage. Our finding suggests that reduction of C3 expression by MSCs can help to ameliorate ischemic brain damage, offering a new neuroprotective strategy in stroke therapy. (C) 2016 Published by Elsevier Ireland Ltd.
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