Review
Cell Biology
Kyu Hwan Kwack, Hyeon-Woo Lee
Summary: Dental caries is a common disease that causes damage to teeth and pulp tissue. Dental pulp stem cells have multipotency, high proliferation rate, and immunosuppressive properties, making them ideal for regenerating damaged pulp tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Immunology
Fenyao Li, Xinxin Wang, Jin Shi, Shuting Wu, Wenbo Xing, Yan He
Summary: Dental pulp stem cells have strong immunomodulatory capabilities and can be used to treat inflammation-related diseases and autoimmune disorders. The mechanism of action is complex and may involve the regulation of immune cells through inflammatory immune-related signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Mohammed Zayed, Koichiro Iohara, Hideto Watanabe, Mami Ishikawa, Michiyo Tominaga, Misako Nakashima
Summary: The study found that hypoxia-preconditioned DPSCs (hpDPSCs) have advantages in proliferation rate and stemness, making them a potential effective source for pulp regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Yuyang Chen, Xiayi Wang, Zhuoxuan Wu, Shiyu Jia, Mian Wan
Summary: Dental-derived stem cells, such as DPSCs, SHEDs, SCAPs, DFPCs, and PDLSCs, have excellent proliferation ability and multi-directional differentiation potential. They have significant application prospects in tissue regeneration due to their abundance, biocompatibility, and effectiveness. Epigenetic regulation plays a crucial role in the biological functions of dental-derived stem cells, and their potential for pulp and periodontal regeneration is highly promising.
Article
Chemistry, Multidisciplinary
Qiannan Sun, Zimeng Zhuang, Rushui Bai, Jie Deng, Tianyi Xin, Yunfan Zhang, Qian Li, Bing Han
Summary: Dental pulp stem cells (DPSCs) are ideal seed cells for cartilage tissue engineering due to their easy availability, multi-lineage differentiation ability, and high proliferation ability. This study demonstrates that the histone-modifying enzymes KDM3A and G9A regulate the chondrogenic differentiation of DPSCs by controlling the degradation of SOX9 protein through lysine methylation. KDM3A promotes chondrogenesis by boosting the protein level of SOX9, while G9A hinders chondrogenic differentiation by reducing the protein level of SOX9. The findings provide a theoretical basis to enhance the clinical use of DPSCs in cartilage tissue-engineering therapies.
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Health Care Sciences & Services
Shankargouda Patil, Rodolfo Reda, Nezar Boreak, Hasan Ahmad Taher, Abdulaziz Abu Melha, Ashraf Albrakati, Thilla Sekar Vinothkumar, Mohammed Mustafa, Ali Robaian, Riyadh Alroomy, Rawabi Jaber Ahmed Kharaf, Taif Sharafuddin Kameli, Ahmed Alkahtani, Hosam Ali Baeshen, Vikrant R. Patil, Luca Testarelli
Summary: The study evaluated the adipogenic and osteogenic potential of human dental pulp stem cells after administration of cordycepin, demonstrating significant effects on the adipogenic and osteogenic differentiation of the cells.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Polymer Science
Raul Rosales-Ibanez, Nieves Cubo-Mateo, Amairany Rodriguez-Navarrete, Arely M. Gonzalez-Gonzalez, Tomas E. Villamar-Duque, Leticia O. Flores-Sanchez, Luis M. Rodriguez-Lorenzo
Summary: The study aimed to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. The results of cell viability tests, von Kossa and Alizarin red staining demonstrate the printed scaffolds' ability to support DPSCs attachment, proliferation, differentiation and mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be used for further modeling and preclinical experiments in bone engineering studies.
Article
Dentistry, Oral Surgery & Medicine
Wen-Jin Chen, Jing Xie, Xi Lin, Ming-Hang Ou, Jun Zhou, Xiao-Lang Wei, Wen-Xia Chen
Summary: The study demonstrated that LPS-preconditioned sEVs can enhance the proliferation, migration, angiogenesis, and differentiation of mesenchymal stem cells, leading to structurally improved regenerated tissue similar to normal dental pulp in vivo. These findings suggest that LPS-preconditioned sEVs play a significant role in dental pulp regeneration.
JOURNAL OF ENDODONTICS
(2021)
Article
Oncology
J. F. Huo, M. L. Zhang, X. X. Wang, D. H. Zou
Summary: The study found that chrysin can promote osteogenic differentiation of DPSCs and enhance DPSC-based bone formation in nude mice and rat models. Proteomics analysis revealed the crucial role of Smad3 in this process.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Lina Yu, Liting Zeng, Zeyu Zhang, Guanxiong Zhu, Zidan Xu, Junyi Xia, Jinlong Weng, Jiang Li, Janak Lal Pathak
Summary: This study found that cannabidiol enhances the proliferation, migration, and osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). CBD also exhibits anti-inflammatory, chemotactic, anti-microbial, and tissue regenerative potentials. These findings suggest the potential application of CBD in dental pulp and bone regeneration based on DPSCs.
Article
Cell & Tissue Engineering
Jie Chen, Huaxing Xu, Kun Xia, Shuhua Cheng, Qi Zhang
Summary: In this study, it was found that RvE1 promotes pulp inflammation resolution and dentin regeneration, and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. The effects of RvE1 on DPSCs were mediated at least partially through AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 shows promising application potential in regenerative endodontics.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Keegan Lott, Paris Collier, Marc Ringor, Katherine M. Howard, Karl Kingsley
Summary: The aging populations in many countries are facing the challenge of chronic neurologic conditions such as Parkinson's and Alzheimer's diseases. Research in regenerative medicine is exploring the potential of using mesenchymal stem cells (MSCs), including dental pulp stem cells (DPSCs), to regenerate neurons. This study aimed to determine if the growth factors used in MSC-based studies could induce neuronal differentiation in DPSCs. The results showed that EGF and bFGF could induce differential changes in growth and viability among some rapidly growing DPSCs, leading to the expression of neural differentiation markers.
Article
Biotechnology & Applied Microbiology
Yu Zhu, Shi-min Wei, Kai-xiao Yan, Ying-xin Gu, Hong-chang Lai, Shi-chong Qiao
Summary: The study demonstrated that ADSCs had better osteogenic differentiation potential than DPSCs, and the combined treatment with ADSCs and xenografts was more effective in enhancing bone repair processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
H. Alghutaimel, X. Yang, B. Drummond, H. Nazzal, M. Duggal, E. Raif
Summary: The study focused on investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells both in vitro and in vivo. Findings showed that recellularization with hDPSCs led to enhanced vascularization and expression of angiogenesis markers, with significant improvement in vascularization compared to unseeded groups. This suggests a promising strategy for dental pulp regeneration with the combination of DDP and hDPSCs.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
