Review
Engineering, Biomedical
Junpeng Zhu, Xinwang Wang, Lin Lin, Wen Zeng
Summary: Cardiovascular disease is a major cause of death worldwide, and there is a clinical demand for transplantable blood vessels. 3D bioprinting technology has shown significant progress in synthesizing blood vessel grafts with clinical effects. However, challenges remain in synthesizing vascular valves, small diameter vessels, and capillary networks. This review discusses the progress, limitations, and future prospects of 3D bioprinting in these areas.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Medicine, Research & Experimental
Huihui Zhang, Yilin Wang, Zijun Zheng, Xuerong Wei, Lianglong Chen, Yaobin Wu, Wenhua Huang, Lei Yang
Summary: 3D bioprinting technology can replicate native tissue and organ microenvironments by precisely placing cells in bioinks. However, finding the ideal bioink is a challenge. Organ-derived decellularized ECM (dECM) bioink has optimal biomimetic properties but is non-printable due to poor mechanical properties. Recent studies have focused on improving the printability of dECM bioinks. This review highlights the methods used to produce these bioinks, strategies to improve printability, and advancements in tissue regeneration. Challenges and potential applications are also discussed.
Review
Biotechnology & Applied Microbiology
Haonan Wang, Huaqing Yu, Xia Zhou, Jilong Zhang, Hongrui Zhou, Haitong Hao, Lina Ding, Huiying Li, Yanru Gu, Junchi Ma, Jianfeng Qiu, Depeng Ma
Summary: This article reviews the significance and recent progress of ECM-based biomaterials in 3D bioprinting, as well as their applications in tissue regeneration and cancer research. There is great potential for future development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Rita Sobreiro-Almeida, Manuel Gomez-Florit, Rita Quinteira, Rui L. Reis, Manuela E. Gomes, Nuno M. Neves
Summary: Decellularized kidney ECM-derived bioink shows great potential for bioprinting renal tissue analogs with high cell viability and structural integrity. The tissue-specific matrix can also influence cellular growth and differentiation over time, demonstrating promising translational applications and implications for bioengineering substitutes using decellularized matrices from other tissues.
Review
Engineering, Biomedical
Faraz Fazal, Sakshika Raghav, Anthony Callanan, Vasileios Koutsos, Norbert Radacsi
Summary: This article summarizes recent advancements in bioinks and 3D bioprinting methods for vascular construct fabrication, emphasizing the critical biomechanical properties needed for ideal vascular grafts and outlining various testing methods for evaluation. It highlights occlusive artery disease and cardiovascular disease as major global causes of death, and discusses the limitations of current surgical methods using autologous or synthetic grafts, calling for tissue engineering approaches to meet the urgent need for vascular grafts that can withstand hemodynamic blood pressure and promote growth and remodeling. Among these approaches, 3D bioprinting offers an attractive solution to create patient-specific vessel grafts with biomimetic structures.
Review
Engineering, Biomedical
Preety Rawal, Dinesh M. Tripathi, Seeram Ramakrishna, Savneet Kaur
Summary: Three-dimensional bioprinting technology is emerging to address limitations in current organoid technologies, showing success in printing stem cells and organoids. Vascularization strategies for bioprinted organoids are critical for achieving complex tissue organization. Harnessing and directing advancements in 3D bioprinting is essential for realizing the translational applications of organoids in disease modeling and regenerative medicine.
BIO-DESIGN AND MANUFACTURING
(2021)
Article
Engineering, Biomedical
Min Kyeong Kim, Wonwoo Jeong, Hyun-Wook Kang
Summary: A new liver decellularized extracellular matrix-gelatin composite bioink was developed to improve the printability and mechanical properties of traditional bioinks. The novel bioink showed significantly higher bioprinting resolution and compressive modulus, and enabled stable stacking of multiple layers. It also exhibited excellent compatibility with hepatocytes and endothelial cells. The liver lobule mimetic structure fabricated using the bioink demonstrated excellent hepatic functionalities and dose-dependent responses to hepatotoxic drugs.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Wonbin Park, Ge Gao, Dong-Woo Cho
Summary: This review discusses the advances in musculoskeletal tissue engineering using 3D bioprinting technology and decellularized extracellular matrix bioink. It focuses on in vivo regeneration and in vitro modeling of musculoskeletal tissue, while also addressing current breakthroughs, limitations, and future prospects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Xueting Yang, Yue Ma, Xiuting Wang, Shengmeng Yuan, Fangjun Huo, Genzheng Yi, Jingyi Zhang, Bo Yang, Weidong Tian
Summary: This study successfully designed a 3D bioprinted biomimetic periodontal module using a specific cell-laden bioink, which showed favorable mechanical properties and orientation guidance. The module also provided a conducive biochemical environment for regulating cell behavior. The experiments conducted on animals demonstrated that the bioprinted module significantly enhanced the regeneration of periodontal tissues and opened up new possibilities for future clinical practice.
Review
Biotechnology & Applied Microbiology
Mabel Barreiro Carpio, Mohammadhossein Dabaghi, Julia Ungureanu, Martin R. Kolb, Jeremy A. Hirota, Jose Manuel Moran-Mirabal
Summary: The human lungs present a challenge in modeling due to their intricate hierarchical structure and heterogeneous mechanical properties. Efforts have been made to develop reliable lung tissue models for studying cell dynamics, disease evolution, and drug interactions. 3D bioprinting is highlighted as a promising technology to address current challenges, bridging the gap between cell cultures and living tissues and potentially producing functional tissue for transplantation. Despite current limitations, optimizing bioink composition and viewing the printing process holistically could unlock the unexplored potential of 3D bioprinting technology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Jiahua Zheng, Yibin Liu, Chenxiao Hou, Zhongkang Li, Shaopeng Yang, Xiao Liang, Liang Zhou, Jiangbo Guo, Jingkun Zhang, Xianghua Huang
Summary: This study explores the use of decellularized extracellular matrix (dECM)-based bioink to fabricate 3D structures with primary ovarian cells (POCs) for correcting ovarian failure. The findings suggest that 3D bioprinting of the ovary using dECM-based bioink is a promising approach for repairing ovarian function, as it promotes neovascularization, cell proliferation, and survival, and enhances germ cell expression.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Engineering, Biomedical
Karli A. Gold, Biswajit Saha, Navaneeth Krishna Rajeeva Pandian, Brandon K. Walther, Jorge A. Palma, Javier Jo, John P. Cooke, Abhishek Jain, Akhilesh K. Gaharwar
Summary: The introduction of a new class of nanoengineered hydrogel-based cell-laden bioinks in 3D bioprinting shows promising potential for replicating the microenvironment of human vasculature, with high printability and cell protection. The ability to print anatomically accurate, multicellular blood vessels provides a valuable tool for modeling vascular function and pathophysiology, demonstrating thromboinflammatory responses similar to in vitro and in vivo models.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Meng Wang, Jianping Zhao, Yixuan Luo, Qianyi Liang, Yisi Liu, Gang Zhong, Yin Yu, Fei Chen
Summary: This study reports a method for fabricating mechanically tunable scaffolds for cartilage regeneration using 3D printing. The addition of short fibers improves printing fidelity and generates mechanically strong constructs. By optimizing the composition of the bioink, scaffolds with good biocompatibility and support for chondrogenesis can be achieved. This method has significant potential for various applications.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Dafydd O. Visscher, Hyeongjin Lee, Paul P. M. van Zuijlen, Marco N. Helder, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: The study developed a printable bioink cdECMMA derived from porcine auricular cartilage, which exhibited good rheological properties and mechanical integrity. Chondrocytes in the printed cdECMMA hydrogel maintained viability, proliferation capacity, and produced cartilage ECM components. The novel cell-based bioprinting using this cartilage-specific dECMMA bioink offers an alternative option for auricular cartilage reconstruction.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
Chen Qin, Jingge Ma, Lei Chen, Hongshi Ma, Hui Zhuang, Meng Zhang, Zhiguang Huan, Jiang Chang, Nan Ma, Chengtie Wu
Summary: This study successfully developed a multicellular system based on a bilayered co-culture scaffold for osteochondral regeneration via a 3D bioprinting strategy. The results show the potential of inducing osteochondral tissue regeneration in vivo through the 3D bioprinted co-culture scaffolds.