Article
Biochemistry & Molecular Biology
Aliakbar Alei, Fatemeh Moradian, Ayoub Farhadi, Mehdi Rostami
Summary: The aim of this study was to identify, isolate, characterize, and clone a new extracellular alkaline protease from the native bacterium Bacillus sp. RAM53 that was isolated from rice fields in Iran. The enzyme was successfully produced through culture and extraction, and its optimum temperature, pH, and molecular weight were determined. The protease showed high identity with other alkaline proteases related to Bacillus and has potential applications in various industries.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Anand Javee, Sathuvan Malairaj, Nagaraj Subramani
Summary: This study proposes a substitute method for recovering silver from waste X-ray films, which is more cost-effective and environmentally friendly compared to conventional methods. By optimizing the factors for protease production, a highly stable protease was obtained, which effectively breaks down the gelatin layer.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Review
Biotechnology & Applied Microbiology
Fatema Matkawala, Sadhana Nighojkar, Anil Kumar, Anand Nighojkar
Summary: Proteolytic enzymes play a crucial role in various industrial processes, especially in protein hydrolysis. The recent trend of biotechnological applications has sparked renewed interest in alkaline serine proteases, expanding their applications in different industries. While many microorganisms have been found to produce alkaline serine proteases on a large scale, research is still ongoing to find enzymes with desirable characteristics.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Marwa Elhamdi, Sofiane Ghorbel, Noomen Hmidet
Summary: A novel alkalophilic bacterium, Bacillus swezeyi B2, with proteolytic activity of 1400 U/ml was isolated from contaminated soil. The crude enzyme likely contained a single extracellular protease, which was highly stable and active under alkaline and high temperature conditions. It remained stable in the presence of surfactants, oxidizing agents, and commercial detergents, and showed excellent stain removal efficacy.
CURRENT MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Asma Zafar, Ziaur Rahman, Hira Mubeen, Javairia Makhdoom, Javeria Tariq, Mahjabeen, Zulqurnain Ali, Attia Hamid, Eeza Shafique, Muhammad Nauman Aftab
Summary: This study aimed to clone and express the esterase gene from Bacillus thuringiensis and investigate its purification, stability, and activity. The results showed that the recombinant esterase enzyme had high activity, stability, and resistance, which are significant for the detergent industry.
Article
Agriculture, Multidisciplinary
Shounan Wang, Yibin Xue, Peng Zhang, Qiaojuan Yan, Yanxiao Li, Zhengqiang Jiang
Summary: In this study, the CRISPR/Cas9 system was used to successfully build a multi-copy expression of an alkaline serine protease (AoproS8) from Aspergillus oryzae in Aspergillus niger, resulting in increased yield. The high protease activity of AoproS8 was utilized for the production of XOD-inhibitory peptides. This work provides a useful method for efficient expression of proteases in A.niger and high-value utilization of protein by-products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Zahoor Khan, Maryam Shafique, Nusrat Jabeen, Sehar Afshan Naz, Kousar Yasmeen, Uroosa Ejaz, Muhammad Sohail
Summary: This study investigated the potential of an alkaline serine protease from Bacillus subtilis as a bating agent in leather processing. The enzyme showed promising results in terms of increasing production and improving the quality of leather, while also being environmentally friendly. These findings provide a strong basis for the use of this protease as an efficient alternative in the leather tanneries.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Polson Mahakhan, Patapee Apiso, Kannika Srisunthorn, Kanit Vichitphan, Sukanda Vichitphan, Sukrita Punyauppa-path, Jutaporn Sawaengkaew
Summary: Protease is a widely used enzyme in the detergent industry. This research aimed to isolate alkaline protease-producing bacteria and characterize them as laundry detergent additives. The highest alkaline protease-producing bacterium, identified as Bacillus gibsonii, exhibited stability at a pH range of 7.0-12.0 and a temperature of 45 degrees C. The alkaline protease from B. gibsonii showed efficiency in blood stain removal and could be used as a detergent additive with potential applications in various industries.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chandran Masi, Getachew Gemechu, Mesfin Tafesse
Summary: This study successfully isolated and identified three Bacillus strains with high alkaline protease activity from leather industry effluent in Ethiopia. These strains demonstrate potential commercial value in producing alkaline protease.
ANNALS OF MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Poonam Bansal, Raman Kumar, Jasbir Singh, Suman Dhanda
Summary: An alkaline serine protease from Pediococcus acidilactici NCDC 252 was purified and characterized, exhibiting high hydrolyzing activity and stability. Its in-silico characterization revealed similarity with other serine proteases, indicating potential industrial applications.
Article
Biology
Dilara Abbas Bukhari, Amina Barkat, Abdul Rehman
Summary: The study aimed to isolate bacterial strains producing protease from soil samples, identifying a Bacillus subtilis with high protease activity after gene sequencing. The expressed alkaline protease showed increased enzyme activity, stability at high temperatures, and potential applications in food and biotechnological industries.
Article
Biotechnology & Applied Microbiology
Nihan Arabaci, Tuna Karaytug
Summary: This study successfully isolated an alkaline thermophilic protease from a Bacillus strain, and investigated its usability as a detergent bio-additive. The protease showed high activity and stability, and was able to effectively remove protein-based stains. Therefore, it has the potential to be used as a biological additive in laundry detergents.
INDIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jianyi Zhang, Chunhua Li, Kai Ren, Min Hong, Jiayue Cui, Jiankai Liu
Summary: We studied the inhibitory effect of alkaline serine protease (ASPNJ) on Jurkat cells and its related mechanism by examining the expression of membrane proteins or membrane-associated proteins. ASPNJ significantly inhibited the proliferation of Jurkat cells, caused morphological changes, and induced apoptosis. ASPNJ also increased the sensitivity of Jurkat cells to vincristine. 2DE-MS identified differentially expressed proteins such as Peroxiredoxin-6 (PRDX6), Calcium-binding protein (CHP1), and 40S ribosomal protein SA (RPSA). This study provides new insights for antileukemia research.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chang Chang, Siyi Gong, Zhiping Liu, Qiaojuan Yan, Zhengqiang Jiang
Summary: In this study, an alkaline serine protease gene (GsProS8) from Geobacillus stearothermophilus was successfully cloned and expressed in Bacillus subtilis. The recombinant GsProS8 exhibited high protease activity of 3807 U/mL after fermentation, showing broad substrate specificity and different kinetic parameters to casein and whey protein. The hydrolysis of whey protein using GsProS8 resulted in a large amount of peptides with high angiotensin-I-converting enzyme (ACE) inhibitory activity, suggesting its potential for industrial applications, particularly in the preparation of antihypertensive peptides.
Article
Biotechnology & Applied Microbiology
Askin Tekin, Ugur Uzuner, Kazim Sezen
Summary: In this study, a heterologous expression, enzymatic characterization, and structure homology modeling of a subtilisin-like alkaline serine protease (ASP) from Bacillus halodurans C-125 were reported. The recombinantly produced ASP demonstrated remarkable pH stability, notable thermostability, and high specific activity at extreme alkaline conditions, making it a potential novel protease candidate for various industrial sectors such as textile, detergent, leather, feed, waste, pharmaceutical, and others.
BIOTECHNOLOGY LETTERS
(2021)
