Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments

Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery

(2016) Mehmet Fatih Bolukbasi et al.   NATURE METHODS

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing

(2015) Hongshan Guo et al.   Nature Protocols

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

(2015) Nicholas C.O. Lee et al.   NUCLEIC ACIDS RESEARCH

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

(2015) Wenjun Jiang et al.   Nature Communications

Programmable DNA cleavagein vitroby Cas9

(2015) Tautvydas Karvelis et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish

(2014) Thomas O Auer et al.   Nature Protocols

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Beyond sequencing: optical mapping of DNA in the age of nanotechnology and nanoscopy

(2013) Michal Levy-Sakin et al.   CURRENT OPINION IN BIOTECHNOLOGY

Next generation biofuel engineering in prokaryotes

(2013) Luisa S Gronenberg et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Combinatorial biosynthesis of polyketides—a perspective

(2012) Fong T Wong et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

Direct cloning of large genomic sequences

(2012) Ryan E Cobb et al.   NATURE BIOTECHNOLOGY

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research

(2012) Hong-Bin Zhang et al.   Nature Protocols

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Cloning Large Gene Clusters from E. coli Using in Vitro Single-Strand Overlapping Annealing

(2012) Rui-Yan Wang et al.   ACS Synthetic Biology

Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling

(2011) Hongcang Gu et al.   Nature Protocols

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Ubiquity of Biological Ice Nucleators in Snowfall

(2008) B. C. Christner et al.   SCIENCE