Synthesis, characterization, and in vitro cytotoxic evaluation of hydroxyapatite submicron particles functionalized with 2-chlorobenzoylthiourea as a drug delivery system

In this study, hydroxyapatite (HAp) nanoparticles (NPs) were synthesized via hydrothermal synthesis method. The surfaces of HAp NPs were functionalized with (3-aminopropyl)triethoxysilane (APTES) and 2-chlorobenzoylthiourea (BT) to obtain HAp-APTES-BT NPs. The structural characterization of HAp submicron particles was made via FTIR and X-ray diffraction techniques. HAp- APTES and HAp-APTES-BT submicron particles were characterized via FTIR. The particle size of HAp submicron particles was determined to be 1281, 1484, and 1718 nm, and their zeta potentials were determined to be -3.38, -3.25, and -3.18. Zoledronic acid (ZA) was loaded onto the HAp- APTES-BT submicron particles under supercritical CO2 conditions (25 degree celsius, 200 bar, 1 h). Drug release profiles of HAp-APTES-BT-ZA submicron particles were investigated in phosphate buffered saline solution (pH 7.4, 37 degree celsius) for 72 h. The cytotoxic effects of HAp-APTES-BT-ZA submicron particles on Saos-2 cells were determined by 3-(4,5-dimethylthiazol-2-yl)-diphenyl tetrazolium bromide test.

Automated summary

In this study, hydroxyapatite nanoparticles were synthesized and functionalized. The loaded drug on the nanoparticles was investigated for drug release and cytotoxicity.