Development of SRAP markers to evaluate the genetic variability in Brachypodium distachyon complex

Brachypodium distachyon has been accepted as a model grass species for genetics and molecular genomics in cereals since 2001. However, the genetic variability present in Brachypodium spp. continue being the aim of many research. Therefore, the aim of this work was to analyze the genetic variability present in different Brachypodium spp. from different countries and to find different open reading frames (ORFs) among them by using sequence-related amplified polymorphism (SRAP) markers. In our study, a total of 12 Brachypodium spp. accessions were selected from different countries. By using SRAP markers, the genetic variability was evaluated and some fragments were sequenced to obtain differential ORF information among genotypes. SRAP molecular markers revealed high variability among accessions being USA the accession that showed the most variability at the DNA level. The analysis of sequenced SRAP fragments from monomorphic and polymorphic fragments as well as those obtained from new primer combinations targeted coding regions into B. distachyon available genome. SRAP molecular marker showed not only to be an excellent tool for Brachypodium variability studies but also to provide additional information about differential amplification of open reading frames.

Automated summary

Since 2001, Brachypodium distachyon has been widely accepted as a model grass species for genetics and molecular genomics in cereals. This study aimed to analyze the genetic variability present in different Brachypodium spp. from different countries and identify different open reading frames (ORFs) using sequence-related amplified polymorphism (SRAP) markers. The study found high genetic variability among the 12 selected Brachypodium spp. accessions, with the accession from the USA showing the highest DNA-level variability. Sequenced SRAP fragments revealed both monomorphic and polymorphic fragments, as well as differential amplification in coding regions of the available B. distachyon genome. SRAP markers prove to be an excellent tool for studying Brachypodium variability and providing additional information about differential amplification of open reading frames.