Journal
NATURAL PRODUCT RESEARCH
Volume 30, Issue 14, Pages 1646-1654Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2015.1131982
Keywords
Brassica oleracea; fatty acids; triacylglycerols; carotenoids; tocopherols; polyphenols; HPLC-PDA-ESI-MS
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Funding
- Shimadzu Corporation
- Sigma-Aldrich/Supelco Corporation
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We report an extensive chemical characterisation of fatty acids, triacylglycerols, tocopherols, carotenoids and polyphenols contained in the oil extracted from old cabbage (Brassica oleracea L. var. acephala) by cold-pressing of the seeds. Analyses were performed by GC-FID combined with mass spectrometry, HPLC with photodiode array, fluorescence and mass spectrometry detection. The 94% of the total fatty acids were unsaturated, rappresented by erucic acid (more than 50%) followed by linoleic, linolenic and oleic acids accounting for approximately 10% each. The most abundant triacylglycerols (> 13%) were represented by erucic-gadolenic-linoleic, erucic-erucilinoleic and erucic-erucic-oleic. Among tocopherols, gamma-tocopherol accounted for over 70% of the total content. Thirteen carotenoids and 11 polyphenols were identified and measured. In particular, the total content in carotenoids was 10.9 ppm and all-E-lutein was the main component (7.7 ppm); among polyphenols, six hydroxycinnamic acids and five flavonoids, were identified by combining information from retention times, PDA and MS data. [GRAPHICS] .
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