Journal
NATURAL PRODUCT RESEARCH
Volume 30, Issue 20, Pages 2364-2367Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2016.1169416
Keywords
-D-glycosidase; enzymolysis; preparative liquid chromatography; timosaponin AIII
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Funding
- programmes of the National Natural Science Foundation of China [81173561, 81274200, 81403175]
- Youth Talent Sail Plan from Shanghai Committee of Science and Technology [14YF1411300]
- Project from Shanghai Municipal Commission of Health and Family planning [20134Y053]
- Project from Shanghai Committee of Science and Technology [13401900301]
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Timosaponin AIII (TAIII) exhibits extensive pharmacological activities and has been reported as a potent antitumour agent for various human cancers. In the present study, a potential industrial process for producing TAIII that involves biotransformation directly in the crude extract liquid of rhizoma anemarrhenae (RA) was developed. -D-glycosidase was used to transform timosaponin BII (TBII) into TAIII, and monofactor experiments were conducted to optimise the enzymolysis conditions. In addition, AB-8 macroporous resin column chromatography, preparative liquid chromatography, and crystallisation technique were applied for yielding TAIII crystals with a purity > 97%. Approximately, 7g of TAIII with a high purity of > 97% was obtained from 1kg of RA through this five-step preparation method, which can be used to produce TAIII on a large scale. [GRAPHICS]
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