Journal
NANOSCALE
Volume 8, Issue 42, Pages 18087-18097Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr06203b
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Funding
- Multi-omics program through the NRF - Korean Ministry of Science, ICT and Future Planning [NRF-2012M3A9B9036679]
- Stem cell research program through the NRF - Korean Ministry of Science, ICT and Future Planning [NRF-2015M3A9C7030128]
- GIST Research Institute (GRI)
- KIST grant
- National Research Council of Science and Technology
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The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research.
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