Journal
NANOSCALE
Volume 8, Issue 44, Pages 18635-18639Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr05806j
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Funding
- National Natural Science Foundation of China (NSFC) [21535006]
- National Basic Research Program of China (973 Program) [2011CB933600]
- China Postdoctoral Science Foundation [2015M582493]
- Postdoctoral Science Foundation of Chongqing [xm2015094]
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Tracking virus infection events in live cells is useful for understanding the mechanism of virus infection, and fluorescent labelling is a critical step. Herein a noninvasive strategy for labelling viruses with His-tags was developed by in situ modifying the cell surface proteins with polypeptides containing His-tags during progeny virus assembly. The His-tagged viruses were further conjugated with Ni2+-nitrilotriacetate complex modified quantum dots, and retained their infectivity for real-time single virus tracking in living cells.
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