Journal
NANOSCALE
Volume 8, Issue 33, Pages 15233-15240Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr03199d
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Funding
- Danish National Research Foundation (Centre for DNA Nanotechnology) [DNRF81]
- Sino-Danish Centre for Education and Research
- Carlsberg Foundation
- Karen Elise Jensens Fond
- AUFF NOVA-project
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A spatial arrangement of proteins provides structural and functional advantages in vast technological applications as well as fundamental research. Most protein patterning procedures employ complicated, time consuming and very costly nanofabrication techniques. As an alternative route, we developed a fully biomolecular self-assembly method using DNA Origami Frames (DOF) as a template for both small and large scale protein patterning. We employed a triangular DOF (tDOF) to arrange the Bovine Serum Albumin (BSA) protein. Our in situ protein patterning strategy provides a novel, fully organic platform using a fast and low-cost surface approach with possible utilization in fundamental science and technological applications.
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