Review
Engineering, Manufacturing
Amitava Bhattacharyya, Gopinathan Janarthanan, Insup Noh
Summary: The complexity of biological architectures has led to extensive use of additive manufacturing techniques for tissue engineering scaffolding, with a current focus on cell-laden 3D bioprinting for tissue or organ regeneration. Nanocomposite bioinks have gained attention for their ability to improve structural stability, shape fidelity, and induce differentiation and cell growth through controlled use of nano-biomaterials. The review emphasizes the significant importance of bioactive nanomaterials in bioinks for 3D bioprinting processes, highlighting recent advances and future possibilities in the field.
ADDITIVE MANUFACTURING
(2021)
Review
Pharmacology & Pharmacy
Izeia Lukin, Itsasne Erezuma, Lidia Maeso, Jon Zarate, Martin Federico Desimone, Taleb H. Al-Tel, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Tissue engineering has become a viable medical alternative in response to the increasing lifespan of society. This review focuses on gelatin, a widely used biomaterial in tissue engineering, and discusses ongoing concerns and the latest developments in this field. Advancements in technology, such as 3D bioprinting and gelatin functionalization, have allowed for better replication of native tissue in gelatin-based systems. This breakthrough has implications for both the manufacturing process, making it more practical and cost-effective, and the future use of gelatin in disease detection and diagnosis rather than treatment.
Review
Materials Science, Multidisciplinary
Shivesh Anand, Serena Danti, Lorenzo Moroni, Carlos Mota
Summary: It is estimated that by 2050 one in every ten people will be suffering from disabling hearing loss. Perforated tympanic membranes (TMs) are the most common injury to the human ear, resulting in a partial or complete hearing loss due to inept sound conduction. Tissue engineering approaches, including TM repair and reconstruction, have been developed for TM regeneration.
PROGRESS IN MATERIALS SCIENCE
(2022)
Review
Engineering, Biomedical
Daniel Salthouse, Katarina Novakovic, Catharien M. U. Hilkens, Ana Marina Ferreira
Summary: This review highlights the importance of the immune response to biomaterials and discusses challenges and opportunities in the development and standardized assessment of the immune response to biomaterials, including the importance of endotoxin levels. It also explores the properties of biomaterials that impact the host immune response and the use of immunomodulatory biomaterials in regenerative medicine. Finally, a standardized in vitro pathway for evaluating the immune response to biomaterials (hydrogels) and their regenerative potential is proposed to ensure safety, consistency, and reduce costs and animal use.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
David R. Maestas, Liam Chung, Jin Han, Xiaokun Wang, Sven D. Sommerfeld, Sean H. Kelly, Erika Moore, Helen Hieu Nguyen, Joscelyn C. Mejias, Alexis N. Pena, Hong Zhang, Joshua S. T. Hooks, Alexander F. Chin, James I. Andorko, Cynthia A. Berlinicke, Kavita Krishnan, Younghwan Choi, Amy E. Anderson, Ronak Mahatme, Christopher Mejia, Marie Eric, JiWon Woo, Sudipto Ganguly, Donald J. Zack, Liang Zhao, Edward J. Pearce, Franck Housseau, Drew M. Pardoll, Jennifer H. Elisseeff
Summary: The immune system plays an important role in tissue repair. A regenerative immunotherapy using the helminth Schistosoma mansoni soluble egg antigen (SEA) was developed, which stimulates the production of IL-4 and other type 2-associated cytokines. The application of regenerative SEA (rSEA) promotes accumulation of IL-4-expressing T helper cells, eosinophils, and regulatory T cells, leading to improved repair and reduced fibrosis. Encapsulation and controlled release of rSEA in a hydrogel further enhance tissue repair.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Sunena Dhania, Manju Bernela, Ruma Rani, Minakshi Parsad, Sapna Grewal, Santosh Kumari, Rajesh Thakur
Summary: Tissue engineering technology utilizes biopolymer materials like polyhydroxyalkanoates to mimic and replace damaged tissues, with excellent biocompatibility and biodegradability. This technology has vast potential applications in areas such as bone grafts, cardiovascular repair, and nerve regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biotechnology & Applied Microbiology
Karolina Lawkowska, Clemens Rosenbaum, Piotr Petrasz, Luis Kluth, Krzysztof Koper, Tomasz Drewa, Marta Pokrywczynska, Jan Adamowicz
Summary: Despite tissue engineering studies heralding the introduction of this method into clinics for over a decade, its application in reconstructive urology is still limited.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Immunology
Alok Shiomurti Tripathi, Magdi E. A. Zaki, Sami A. Al-Hussain, Bidhyut Kumar Dubey, Prabhjot Singh, Laiba Rind, Rajnish Kumar Yadav
Summary: Biomaterials are widely used in various medical applications, but their introduction into the body can cause immune responses. The immune system recognizes natural biomaterials as foreign substances, activating immune cells and leading to inflammation. The surface properties and released immunomodulatory factors of biomaterials can influence immune cell behavior, and a better understanding of this interaction can help design improved biomaterials.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Francisco Vieira dos Santos, Renato Luiz Siqueira, Lucas de Morais Ramos, Sergio Akinobu Yoshioka, Marcia Cristina Branciforti, Daniel Souza Correa
Summary: This review highlights the versatile application of silk fibroin (SF)-derived electrospun materials in tissue regeneration, disease treatment, and drug delivery systems, as well as explores potential future trends and limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Engineering, Biomedical
Vishnu Priya Murali, Christina A. Holmes
Summary: This review summarizes various biomaterial-based extracellular vesicle (EV) delivery systems used in preclinical tissue regeneration studies and other therapeutic applications. EV-based therapies have shown success in preclinical models for biomedical applications, but the challenge lies in prolonging EV retention at target sites for improved therapeutic efficacy through biomaterial-based systems.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Lingfei Ren, Zhiwei Jiang, Hui Zhang, Yani Chen, Danji Zhu, Jin He, Yunxuan Chen, Ying Wang, Guoli Yang
Summary: The hard palate mucosa (HPM) serves as an autologous donor area with superior biosafety and trauma healing ability, making it widely used and researched. Specific mesenchymal stem cells have been isolated from HPM, which have played an essential role in various clinical applications.
MATERIALS TODAY BIO
(2023)
Article
Green & Sustainable Science & Technology
Mano Govindharaj, Noura Sayed Al Hashemi, Soja Saghar Soman, Sanjairaj Vijayavenkataraman
Summary: This study successfully identified an environmentally destructive tunicate species and proposed sustainable utilization of tunicate tunics as wound-dressing materials, decellularized extracellular matrix (dECM) scaffolds, and bioinks. The tunics showed excellent biocompatibility and tensile modulus. The findings of this work contribute to the preservation of marine ecosystems and the reduction of costs associated with tunicate management.
JOURNAL OF CLEANER PRODUCTION
(2022)
Review
Pharmacology & Pharmacy
Roisin Lynch, Ed C. Lavelle
Summary: Biocompatible and biodegradable biomaterials are widely used in regenerative medicine. Inflammation, though initially essential for wound healing, can have negative effects if prolonged. Therefore, careful consideration should be given to the choice of biomaterials to minimize inflammation.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Agnese Gugliandolo, Luigia Fonticoli, Oriana Trubiani, Thangavelu S. Rajan, Guya D. Marconi, Placido Bramanti, Emanuela Mazzon, Jacopo Pizzicannella, Francesca Diomede
Summary: Tissue engineering has become a major focus of research, with the potential of biomaterials and mesenchymal stem cells (MSCs) showing promise for bone regeneration, particularly in oral bone regeneration. Combining MSCs with biomaterials enhances bone regenerative power, and utilizing the MSC secretome also presents a promising future for bone tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Thiago Domingues Stocco, Mayara Cristina Moreira Silva, Marcus Alexandre Finzi Corat, Gabriely Goncalves Lima, Anderson Oliveira Lobo
Summary: This study presents a new biofabrication strategy for developing biomimetic meniscus scaffolds. The use of bioprinted cell-laden hydrogel reinforced by aligned nanofiber sheets improved the structural integrity, shape fidelity, and mechanical properties of the scaffolds. Importantly, the introduction of nanofiber mats did not negatively affect cell viability. This bioengineering approach offers a promising strategy for tissue engineering of the knee meniscus.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Cell & Tissue Engineering
Kieran Joyce, Zaklina Buljovcic, Goran Rosic, Marietta Kaszkin-Bettag, Abhay Pandit
Summary: This article evaluates the regulatory landscape of tissue-engineered products (TEPs) and identifies the trends in clinical trial activity in the European Union (EU). The study highlights the most active member states, commercial involvement, a shift toward the use of allogeneic cells, and a renewed interest in TEP development.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Neurosciences
Vaibhav Patil, Raghvendra Bohara, Carla Winter, Michelle Kilcoyne, Siobhan McMahon, Abhay Pandit
Summary: This study investigated the glycosylation modulation in a mixed glial culture model and identified considerable differences in glycosylation from acute to chronic phases. Clustering analysis revealed dense clusters for control and LPS treated groups, while the cytokine combination group was scattered into different clusters. Lectin immunostaining confirmed the presence of various glycans in astrocytes and microglia, including GalNAc, GlcNAc, mannose, fucose, and sialic acid-binding residues. Inhibition of sialyltransferase suppressed the upregulation of sialic acid expression, resulting in modulation of mitochondrial functions.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Yazhong Bu, Wenwen Zhang, Sergio Martin-Saldana, Amir M. Alsharabasy, Mark Da Costa, Luyao Feng, Ze Zhang, Xinlan Ge, Chonghui Li, Shichun Lu, Abhay Pandit
Summary: This study develops a series of multifunctional bioadhesives using succinic anhydride-modified hyaluronic acid (HASA) and plant-derived tannic acid (TA) with centrifuging and freeze-drying. By varying the ratio of HASA to TA, HASATA 1:2, 1:7, and 1:14 are fabricated with tunable adhesive strength. The bioadhesives accelerate wound healing, exhibit antioxidant properties, and have antibacterial activity, making them suitable for noninvasive wound closure in the clinical setting.
ADVANCED MATERIALS INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Sean O'Halloran, Abhay Pandit, Andreas Heise, Andrew Kellett
Summary: Two-photon polymerization (TPP) is a leading method for microscale fabrication of customized polymeric devices and surfaces. It has a wide range of applications in optics, drug delivery, tissue engineering, and microfluidics. TPP has evolved from standard acrylate- and epoxy-based photoresists to custom formulated monomers that can modify the properties of resulting structures. This article provides an overview of the essential principles and advanced chemical modification strategies for TPP materials and introduces commonly used photoresists and their applications.
Article
Clinical Neurology
Rosanna Rossi, Andrew Douglas, Sara Molina Gil, Duaa Jabrah, Abhay Pandit, Michael Gilvarry, Ray McCarthy, James Prendergast, Katarina Jood, Petra Redfors, Annika Nordanstig, Erik Ceder, Dennis Dunker, Jeanette Carlqvist, Istvan Szikora, John Thornton, Georgios Tsivgoulis, Klearchos Psychogios, Turgut Tatlisumak, Alexandros Rentzos, Karen M. M. Doyle
Summary: By analyzing 122 thrombi from 80 patients with acute ischemic stroke, it was found that elevated S100b levels were associated with an increased risk of PTIH. Additionally, higher S100b expression, higher NIHSS scores at admission, and a greater number of passes during thrombectomy were all associated with PTIH.
FRONTIERS IN NEUROLOGY
(2023)
Review
Biochemistry & Molecular Biology
Caglar Ersanli, Athina Tzora, Ioannis Skoufos, Chrysoula (Chrysa) Voidarou, Dimitrios I. Zeugolis
Summary: Biomaterial-based therapies are being explored to combat drug-resistant bacterial strains and off-target impacts of conventional strategies in treating microbial infections. Collagen, a fibrous, non-soluble protein, has been extensively studied for the development of antimicrobial biomaterials due to its superior properties. This review focuses on the various approaches used to develop collagen-based antimicrobial devices, including non-pharmacological, antibiotic, metal oxide, antimicrobial peptide, herbal extract-based, and combination approaches, with a particular emphasis on preclinical studies from the past decade.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Paolo Contessotto, Renza Spelat, Federico Ferro, Vaidas Vysockas, Ausra Krivickiene, Chunsheng Jin, Sandrine Chantepie, Clizia Chinello, Audrys G. Pauza, Camilla Valente, Mindaugas Rackauskas, Alvise Casara, Vilma Zigmantaite, Fulvio Magni, Dulce Papy-Garcia, Niclas G. Karlsson, Egle Ereminiene, Abhay Pandit, Mark Da Costa
Summary: The lack of a reproducible pre-clinical model hinders the study of the pathophysiology and possible interventions for non-ST-segment elevation myocardial infarction (NSTEMI). In this study, authors develop an ovine model to reproduce post-ischemic remodeling in NSTEMI and reveal distinct complex sugar moieties in cellular membranes and extracellular matrix patterns in infarcted tissue.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
Caglar Ersanli, Athina Tzora, Ioannis Skoufos, Konstantina Fotou, Eleni Maloupa, Katerina Grigoriadou, Chrysoula (Chrysa) Voidarou, Dimitrios I. Zeugolis
Summary: The increase in antimicrobial resistance is a serious public health problem, and there is a need for alternative antimicrobial agents. Essential oils extracted from aromatic and pharmaceutical plants contain bioactive compounds that can positively affect human and animal health. This study evaluated the antimicrobial and anti-biofilm activity of Thymus sibthorpii, Origanum vulgare, Salvia fruticosa, and Crithmum maritimum essential oils against Staphylococcus aureus strains. The results showed that Thymus sibthorpii and Origanum vulgare could be potential alternatives as antimicrobial agents to overcome microbial resistance.
Article
Engineering, Biomedical
Eugenia Pugliese, Ignacio Sallent, Sofia Ribeiro, Alexandre Trotier, Stefanie H. Korntner, Yves Bayon, Dimitrios I. Zeugolis
Summary: A three-layer scaffold was designed to mimic the matrix composition of the enthesis and loaded with bioactive molecules to promote cell differentiation. The cells differentiated towards osteogenic and fibrocartilage lineages in response to the scaffold composition. Compartmentalised scaffolds show potential for repairing and regenerating the enthesis.
MATERIALS TODAY BIO
(2023)
Review
Biology
Caglar Ersanli, Athina Tzora, Chrysoula (Chrysa) Voidarou, Stylianos Skoufos, Dimitrios I. Zeugolis, Ioannis Skoufos
Summary: Cutaneous wound healing is a complex process that can be affected by the penetration of microorganisms, leading to imbalances in the skin microbiota. Understanding the major bacteria species in wounds and their interaction with the immune system is crucial for wound healing.
Article
Engineering, Biomedical
Caglar Ersanli, Chrysoula (Chrysa) Voidarou, Athina Tzora, Konstantina Fotou, Dimitrios I. Zeugolis, Ioannis Skoufos
Summary: This article briefly reviews preclinical studies on electrospun nanofibrous scaffolds as delivery systems for herbal extracts, with a particular focus on wound-healing applications in the past five years. The indirect effects of herbal extracts on wound healing by altering the characteristics of electrospun mats are also discussed.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Chemistry, Medicinal
Amir M. Alsharabasy, Amal Aljaabary, Raghvendra Bohara, Pau Farras, Sharon A. Glynn, Abhay Pandit
Summary: The study demonstrates that hemin can be used as a potential anticancer agent by scavenging nitric oxide (NO), inhibiting cancer cell migration, and regulating the expression of migration-associated proteins.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Materials Science, Biomaterials
Mangesh Morey, Aitor Larranaga, Sunny Akogwu Abbah, Raghvendra Bohara, Amal Aljaabary, Abhay Pandit
Summary: Nucleic acid therapy has the potential to promote wound healing through gene expression modification, but protecting the nucleic acid payload and achieving efficient bioresponsive delivery remain challenging. In this study, a glucose-responsive gene delivery system was designed using fibrin-coated polymeric microcapsules, which effectively loaded and released multiple nucleic acids with no cytotoxic effects in vitro. When applied to diabetic wounds, the system improved wound healing and upregulated key proteins involved in the process. This hydrogel-based system shows promise for promoting wound healing and can be encapsulated with different therapeutic nucleic acids.
Meeting Abstract
Cell & Tissue Engineering
Honorata Kraskiewicz, Piotr Hinc, Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Maria Paprocka, Danuta Witkowska, Abhay Pandit, Aleksandra Klimczak
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Jack Schofield, Thomas Ritter, Abhay Pandit
TISSUE ENGINEERING PART A
(2023)
