Journal
NANOMEDICINE
Volume 11, Issue 16, Pages 2073-2082Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2016-0071
Keywords
short RNA analyte; SOI-FET biosensor; surface modification
Funding
- Presidium RAS Program [FIMT 2014-154]
- Russian Federation [SP-3752.2015.4, 14.607.21.0125]
- RFBR [16-04-01029, 16-34-00782]
- Basic Program of Research of State Academies of Sciences
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Aim: A new type of surface modification of SOI-FET sensors with ultrathin sensor-probe transition layer and uncharged probes for highly specific detection of short RNA (saRNA) was suggested. Materials & methods: Carbonyldiimidazole (CDI) or glycidoxypropyltrimethoxysilane were used as precursors of sensor-probe interface layers, together with peptide nucleic acids and new NA analogues - phosphoryl guanidine oligo(2'-OMe) ribonucleotides (PGO) as probes for RNA hybridization. RNA sequences corresponding to mRNA NELFA (NM_005663) and microRNA-29a (cancer markers) were used as saRNA targets. Real-time saRNA detection by SOI-FET sensors and fluorescence analysis were applied. Results: Highly specific response with femtomolar sensitivity to saRNA was demonstrated for CDI-PGO-modified sensors fabricated by optical lithography. Conclusion: The proposed CDI-PGO protocol of modification of Si sensor surface is a promising procedure for biomedical applications.
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