Article
Immunology
Yi-Yi Qian, Hong-Yu Wang, Yang Zhou, Hao-Cheng Zhang, Yi-Min Zhu, Xian Zhou, Yue Ying, Peng Cui, Hong-Long Wu, Wen-Hong Zhang, Jia-Lin Jin, Jing-Wen Ai
Summary: Metagenomic next generation sequencing (mNGS) has shown higher sensitivity and better diagnostic performance in detecting pathogens in pulmonary infections, especially for rare and emerging pathogens. It provides reliable phylogenetic and epidemiological information, with a shorter turnaround time, and can complement traditional methods to improve the identification of respiratory infection agents.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Respiratory System
Siqin Zhang, Jing Ou, Yuxue Tan, Bin Yang, Yaoyao Wu, Lin Liu
Summary: This study evaluated the diagnostic performance of metagenomic next-generation sequencing (mNGS) in diabetic patients with pulmonary infections. The results showed that mNGS had a significantly higher microbial positive rate than conventional testing methods, especially in bacterial detection. Klebsiella pneumoniae was the most common pathogen identified by mNGS in patients with diabetes. mNGS is a valuable tool for etiological diagnosis of pulmonary infections in diabetic patients.
BMC PULMONARY MEDICINE
(2023)
Article
Medicine, General & Internal
Xiaoxu Ma, Suping Zhang, Haizhou Xing, Huiling Li, Jiajun Chen, Haijun Li, Mengfan Jiao, Qingmiao Shi, Aiguo Xu, Lihua Xing, Weijie Cao
Summary: Invasive pulmonary aspergillosis is a major cause of morbidity and mortality in immunocompromised patients. This study demonstrates the effectiveness of using peripheral blood metagenomic next-generation sequencing for the rapid and non-invasive diagnosis of invasive pulmonary aspergillosis.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Chaofan Zhang, Yunzhi Lin, Changyu Huang, Zida Huang, Xinyu Fang, Guochang Bai, Zeyu Zhang, Wenbo Li, Wenming Zhang
Summary: This study retrospectively analyzed the clinical features and surgical outcomes of FOI patients in our institution, finding that most patients required multiple surgeries and long-term antibiotic treatment, and indicated that the use of mNGS assisted in the diagnosis and treatment of FOI. Most patients needed multiple surgeries followed by long-term antifungal treatment, with an overall success rate of 100%.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Yuqian Chen, Wei Feng, Kai Ye, Li Guo, Han Xia, Yuanlin Guan, Limin Chai, Wenhua Shi, Cui Zhai, Jian Wang, Xin Yan, Qingting Wang, Qianqian Zhang, Cong Li, Pengtao Liu, Manxiang Li
Summary: Metagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage (BAL) samples showed high diagnostic sensitivity and negative predictive value for pulmonary infections, as well as an advantage in describing the respiratory microbiome changes in patients with different underlying diseases. This method has the potential to play a crucial role in the future clinical use of antimicrobial agents.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Wenjing Tang, Yu Zhang, Chong Luo, Lina Zhou, Zhiyong Zhang, Xuemei Tang, Xiaodong Zhao, Yunfei An
Summary: This study evaluated the utility of mNGS for detecting pathogens in patients with PID, demonstrating its superiority over conventional methods in pathogen identification, especially in detecting rare opportunistic pathogens. Compared to traditional methods, mNGS can provide faster pathogen diagnosis, enabling clinicians to make more timely and targeted therapeutic decisions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Martin Hoenigl, Matthias Egger, Jessica Price, Robert Krause, Juergen Prattes, P. Lewis White
Summary: Timely diagnosis remains an unmet need in non-neutropenic patients at risk for aspergillosis, including those with COVID-19-associated pulmonary aspergillosis (CAPA). Currently available mycological tests show limited sensitivity when testing blood specimens. Metagenomic next-generation sequencing (mNGS) to detect microbial cell-free DNA (mcfDNA) in plasma might overcome some of the limitations of conventional diagnostics.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Immunology
Liang Xu, Zheng Zhou, Yao Wang, Chao Song, Hongdong Tan
Summary: This study evaluated the potential value of metagenomic next-generation sequencing (mNGS) in diagnosing spinal infection. The results showed that mNGS had better performance in pathogen identification compared to conventional microbiological tests (CMTs), and discovered new pathogens. The sensitivity and specificity of mNGS in diagnosing spinal infection were higher than CMTs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Kaipeng Jia, Shiwang Huang, Chong Shen, Hongjun Li, Zhe Zhang, Lei Wang, Gangjian Zhao, Zhouliang Wu, Yuda Lin, Han Xia, Mingze Tang, Huifen Yang, Hailong Hu
Summary: mNGS technology has clear advantages over culture in detecting pathogens and guiding treatment strategies for urinary tract infections (UTIs). It helps improve diagnosis and treatment of difficult-to-diagnose and treat UTIs, and is an effective complement to urine culture.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medical Laboratory Technology
Chendi Jing, Hongbin Chen, Yong Liang, Ying Zhong, Qi Wang, Lifeng Li, Shijun Sun, Yifan Guo, Ruobing Wang, Zhi Jiang, Hui Wang
Summary: The mNGS workflow showed a high clinical sensitivity of 87.1%, improved clinical specificity of 80.2%, and reduced false-positive results by utilizing multiple control groups. This optimization can enhance the utility of mNGS as a powerful tool for identifying pathogens in bloodstream infections.
CLINICAL CHEMISTRY
(2021)
Article
Microbiology
Ziqian Qin, Yiwu Zou, Zehe Huang, Ning Yu, Zhenfeng Deng, Zhencheng Chen, Yuanli Wang
Summary: In this study, we demonstrated that mNGS could accurately distinguish Cryptococcus from M. tuberculosis in pulmonary infection, and it was capable of swiftly and precisely detecting pathogens in mixed bacterial and fungal pulmonary infection. The results of mNGS also have the potential to adjust anti-infective therapies.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Infectious Diseases
Guijuan Xie, Bo Zhao, Xun Wang, Liang Bao, Yiming Xu, Xian Ren, Jiali Ji, Ting He, Hongqing Zhao
Summary: mNGS is a valuable tool for the detection of pulmonary infections, especially in cases of mixed infections. The most common combinations found were bacterial-fungal coinfection and bacterial-bacterial coinfection. Challenges still exist in the clinical application of mNGS in the diagnosis of pulmonary infections, particularly in interpreting the reports and refining clinical judgment and literature analysis strategies.
INFECTIOUS DISEASES AND THERAPY
(2021)
Article
Immunology
Ying Liu, Huifen Wang, Yaoguang Li, Zujiang Yu
Summary: The study aimed to evaluate the clinical diagnostic value of metagenomic next-generation sequencing (mNGS) for tuberculosis (TB). Retrospective analysis of 52 patients with suspected TB infection was conducted, and multiple detection methods were performed. The results showed that mNGS had a higher detection rate of MTB compared to acid-fast staining and PCR, but no statistical difference with T-SPOT.TB assay. As an adjunctive diagnostic technology, mNGS can be combined with traditional methods to guide the diagnosis and treatment of TB.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Jing Zhang, Guoliang Xu, Jiumei Shen, Gangfu Ye
Summary: Fungal infection is common and often misdiagnosed due to its various types and similar symptoms. A case report presents a 32-year-old female diagnosed with a rare pulmonary fungal infection, Schizophyllum commune, through Metagenomic Next-Generation Sequencing (mNGS). The patient was successfully treated with voriconazole and itraconazole, with no recurrence during follow-up.
Article
Immunology
Dengfeng Wu, Wei Wang, Qiufen Xun, Hongluan Wang, Jiarong Liu, Ziqing Zhong, Chao Ouyang, Qing Yang
Summary: This study evaluated the diagnostic performance of metagenomic next-generation sequencing (mNGS) in patients with suspected pulmonary infection and found that mNGS can greatly enhance the accuracy and detection rate of pathogens. Comorbidities and types of pathogens should be taken into consideration when interpreting the results of mNGS.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)