Article
Chemistry, Multidisciplinary
Miaomiao Liu, Yunhong Song, Yi-Heng P. Job Zhang, Chun You
Summary: A method for carrier-free immobilization of multi-enzyme complexes with more than four enzymes is developed using polypeptide interactions and enzyme component self-oligomerization. The self-assembled enzyme complexes show higher initial reaction rates than the four-enzyme cocktail. Water-insoluble self-assembled multi-enzyme complexes are observed, serving as carrier-free immobilized multi-enzyme complex aggregates. These immobilized enzyme complexes can be easily recycled and obtained from either purified enzyme components or crude cell extracts. This strategy sheds light on improving the catalytic capability of in vitro synthetic enzymatic biosystems.
Review
Engineering, Chemical
Hannaneh Rasouli, Kaven Nguyen, Maria C. Iliuta
Summary: Currently, immobilization of the enzyme carbonic anhydrase (CA) is considered a promising approach to enhance the activity, stability, and reusability of CA for CO2 capture. Different immobilization strategies and supports have been developed to improve the performance and efficiency of large-scale applications. This state-of-the-art review analyzes the scientific literature on immobilization of CA and carbon capture technologies using immobilized CA, highlighting the benefits, issues, and limitations. Modelling approaches and techno-economic analyses are also presented to assess the feasibility of enzymatic capture processes in an industrial context.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Guanzhang Liu, Hang Yuan, Xiaobo Li, Ke Li, Lei Mao, Guangya Zhang
Summary: This study proposes an all-in-one strategy to address the challenges of carbon dioxide capture, conversion, and utilization using carbonic anhydrase (CA) as a green and sustainable method. The strategy involves the self-assembly of nanosized CA oligomeric particles (nCAOPs) into micrometer-sized CA supraparticles (mCASPs) with well-designed tags. The prepared mCASPs, with a simple and effective purification method, exhibited high yields and excellent enzymatic properties. Furthermore, the mCASPs could serve as carrier-free immobilized CAs, and the nCAOPs formed from them showed excellent catalytic performances.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Agricultural Engineering
R. Reshmy, Eapen Philip, Ranjna Sirohi, Ayon Tarafdar, K. B. Arun, Aravind Madhavan, Parameswaran Binod, Mukesh Kumar Awasthi, Sunita Varjani, George Szakacs, Raveendran Sindhu
Summary: Nanobiocatalysts, produced by integrating advanced biotechnology and nanotechnology, show great potential in improving enzyme stability, function, and efficiency in bioprocessing. Functional nanostructures are used to create nanobiocatalysts, playing a critical role in controlling enzyme activity. Their enormous potential in designing effective laboratory trials and applications in various fields is extensively discussed.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Simone Adorinni, Giulio Goti, Lorenzo Rizzo, Federica Grassi, Slavko Kralj, Fatima Matroodi, Mirco Natali, Rita De Zorzi, Silvia Marchesan, Luca Dell'Amico
Summary: The conjugation of photoactive benzophenone with diphenylalanine resulted in a self-assembling photocatalyst, which was investigated in the E -> Z photoisomerization of stilbene derivatives.
CHEMICAL COMMUNICATIONS
(2023)
Review
Engineering, Chemical
Gizem Oelcuecue, Ulrich Krauss, Karl-Erich Jaeger, Joerg Pietruszka
Summary: The combination of biocatalysis and flow chemistry shows great potential in the development of efficient and environmentally friendly industrial processes. This review provides an overview of immobilization strategies suitable for flow chemistry, with a focus on recently developed carrier-free immobilization methods, highlighting advances in the field and presenting future trends.
CHEMIE INGENIEUR TECHNIK
(2023)
Article
Biochemistry & Molecular Biology
Meng Qiao, Junjie Zhang, Jingmin Li, Lu Xing, Xue Zhou, Yunchang Xie, Xing Zhang
Summary: This study successfully constructed an expression system and immobilized the enzymes, obtaining high expression levels and good water solubility for hyaluronic acid synthase and chondroitin synthase. The use of metal-organic frameworks as carriers efficiently catalyzed the synthesis of hyaluronic acid and chondroitin, with good stability and reusability.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Luis Gerardo Ramirez-Ramirez, David Enrique Zazueta-Alvarez, Hector Alonso Fileto-Perez, Damian Reyes-Jaquez, Cynthia Manuela Nunez-Nunez, Juan De Dios Galindo-De la Rosa, Javier Lopez-Miranda, Perla Guadalupe Vazquez-Ortega
Summary: This research aimed to immobilize a recombinant beta-glucosidase from Trichoderma reesei using a commercial zeolite. The immobilized enzyme showed improved catalytic characteristics, stability, and a lower KM value compared to the free enzyme. The immobilization parameters obtained through simple adsorption show promising applications in various fields.
Article
Chemistry, Physical
Oliver Pauli, Achim Ecker, Alvaro Cruz-Izquierdo, Alessandra Basso, Simona Serban
Summary: A novel FT-IR microscopy method was developed to analyze the diffusion of lipase CalB in different resins. The study found that the kinetic of immobilization, distribution, and activity of the enzyme varied between the hydrophobic and hydrophilic resins.
Article
Biochemistry & Molecular Biology
Yan Liu, Jingyi Yang, Ke Wang, Feiyu Duan, Lili Lu
Summary: This study established a method for batch synthesis of alpha-GOSs using immobilized enzymes, achieving high activity recovery and stability in the resulting biocatalyst. The immobilized enzyme product showed advantages in storage and reuse.
Article
Chemistry, Multidisciplinary
Martin D. Gelenter, Ad Bax
Summary: Post-translational modifications are commonly found in eukaryotic proteomes. However, these modifications are rarely studied using NMR in eukaryotic proteins, which are usually produced in E. coli through recombinant expression. The native C-terminal amide of melittin, a major peptide in honey bee venom, significantly affects its structure and dynamics. This study presents a method to produce isotopically labeled native melittin through recombinant expression and chemical amidation. It also confirms the accuracy of structural models generated by AlphaFold-Multimer using experimental residual dipolar couplings.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Inorganic & Nuclear
Maniya Gharib, Leili Esrafili, Ali Morsali, Christophe M. L. Vande Velde, Zhifang Guo, Peter C. Junk
Summary: A new porous metal-organic framework was successfully synthesized, showing high performance in CO2 adsorption and conversion. The catalytic efficiency was enhanced by introducing a highly acidic ligand through a postsynthesis method.
INORGANIC CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Anamul Hoque Bhuiyan, Takuma Nagakawa, Mohammad Zakaria, Koji Nakane
Summary: An enzyme-immobilized PVB-Zr hybrid tube was developed and shown to exhibit stability and higher activity in different environments compared to free enzymes. The Km value of immobilized enzymes was slightly higher, but they retained activity over multiple reaction cycles. Furthermore, immobilized lipase in organic solvents demonstrated high catalytic conversion efficiency.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Sudip Bhattacharjee, Sayantan Chongdar, Arindam Modak, Piyali Bhanja, Bikash Kumar Jena, Asim Bhaumik
Summary: A novel microporous zinc phosphonate catalyst (ZnGLy) was synthesized and showed excellent catalytic performance for the chemical fixation of CO2 into mono- to tetra-cyclic carbonates. It also displayed good recyclability.
Article
Chemistry, Multidisciplinary
Piera De Santis, Noemi Petrovai, Lars-Erik Meyer, Markus Hobisch, Selin Kara
Summary: UPOs have been extensively studied in the past decade, with immobilization of the enzyme offering increased stability and resistance, effectively addressing challenges in industrial applications.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Chang Liu, David L. Steer, Haipeng Song, Lizhong He
Summary: This study discovered a novel approach to enhance the affinity between SBPs and silica surfaces by altering the relative orientation of amino acids. It provides a new controllable method for protein immobilization.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Nanoscience & Nanotechnology
Yiyi Liu, Xiaoyu Chen, Xiangfeng Lai, Christian K. O. Dzuvor, Letian Lyu, Seong Hoong Chow, Lizhong He, Lei Yu, Yajun Wang, Jiangning Song, Hsien-Yi Hsu, Tsung-Wu Lin, Philip Wai Hong Chan, Hsin-Hui Shen
Summary: This study presents a new class of furoxan-based NO-releasing nanoparticles that are coassembled with Pluronic F127, which exhibit potent antimicrobial activities against MRSA strains. The coassembled nanoparticles also demonstrate stronger interactions with lipid bilayers and have strong plasma membrane-damaging capabilities. This coassembly approach can be applied to other small molecule-based antimicrobials.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
Zongsheng Zou, Tayyaba Younas, Geoff Dumsday, Victoria S. Haritos, Lizhong He
Summary: RNA aptamers have the ability to specifically and selectively bind to various macromolecules, cell surfaces, and viruses, and find wide applications in biosensors, diagnostics, and therapeutic treatments and drug delivery. This article presents a new E. coli-based approach for rapidly producing multimeric RNA aptamer transcripts in vivo, using a designed double-stranded spacer to protect the transcript ends. The produced multimeric and fluorescent RNA aptamers were stable and easily isolated, and their full functionality was confirmed through binding assays and fluorescence measurements. This approach shows promise for rapid and cost-effective production of RNA aptamers.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Applied
Smriti Shrestha, Leonie van 't Hag, Victoria S. Haritos, Sushil Dhital
Summary: This review explores the potential of lentil and mungbean proteins as plant-based protein sources, which have comparable protein contents but different protein composition compared to soy and pea proteins. Lentil protein exhibits comparable gelling, emulsifying, and superior foaming properties to pea and soy protein, while the gelling, water holding, and oil absorption capacities of mungbean proteins are comparable to soy.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Multidisciplinary
Tayyaba Younas, Chang Liu, Weston B. Struwe, Philipp Kukura, Lizhong He
Summary: Researchers have engineered light-responsive RNA-protein nanowires that exhibit non-covalent light-switching ability. By utilizing the high affinity between RNA and protein, they replaced green fluorescence protein with an optogenetic protein pair system, allowing blue light-controlled photo-switching. These RNA-protein structures can serve as biocompatible frameworks for incorporating versatile elements such as RNA, DNA, and enzymes.
Article
Chemistry, Multidisciplinary
Christian K. O. Dzuvor, Bhuvana K. Shanbhag, Hsin-Hui Shen, Victoria S. Haritos, Lizhong He
Summary: Protein-based nanomaterials have outstanding properties and wide applications in biomedical and bionanotechnological sectors. However, the development of unique nanostructures for next-generation antibacterial agents has been limited. This study reports the discovery of protein nanospears, which are supramolecular nanostructures with well-defined shapes based on engineered proteins. The nanospears exhibit exceptional antibacterial activities, rapidly damaging bacterial morphology via their nanostructure and enzymatic action. These protein-based nanospears show promise as potent tools to combat drug-resistant bacteria and inspire the engineering of other antibacterial protein nanomaterials with diverse properties.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Applied
Smriti Shrestha, Leonie van't Hag, Victoria Haritos, Sushil Dhital
Summary: Lentils and mungbean proteins have been less studied compared to pea and soybean proteins. The proteins of lentils, mungbean, and yellow pea were extracted using alkaline extraction and isoelectric precipitation, and their molecular and higher-order structures were investigated using various methods. The extracted proteins had similar protein content but higher nitrogen solubility index compared to commercial pea and soy protein isolates. The soluble proteins of lentils and yellow pea were identified as legumin-like and vicilin-like, while mungbean proteins were dominated by vicilin-like proteins. The soluble extracts showed native structural characteristics unlike the commercial extracts, and there were also differences in secondary structure between the extracts.
Article
Engineering, Chemical
Li Ji, Puja Paul, Bhuvana K. Shanbhag, Ian Dixon, Shibo Kuang, Lizhong He
Summary: This review summarizes the emerging applications of hydrocyclones in food and bioproduct processing. While hydrocyclones are efficient in removing larger particles such as mammalian cells, separating small cells like yeast is more challenging. Strategies such as decreasing body diameters and increasing inlet velocities have been explored to tackle this challenge at the cost of increasing the water split at underflow and reducing the concentration ratio.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biology
Kevin H. Putera, Jinhee Kim, So Young Baek, Stephen H. Schlecht, Melanie L. Beaulieu, Victoria Haritos, Ellen M. Arruda, James A. Ashton-Miller, Edward M. Wojtys, Mark Banaszak M. Holl
Summary: Approximately 300,000 ACL tears occur annually in the US, with 50% leading to knee osteoarthritis within a decade. Repetitive loading causes collagen unravelling and structural failure, but the relationship between tissue changes is poorly understood. This study shows that submaximal loading of cadaver knees leads to collagen unravelling and tissue compliance increase, particularly in highly mineralized regions at the ACL femoral enthesis.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Applied
Smriti Shrestha, Leonie van 't Hag, Victoria Haritos, Sushil Dhital
Summary: The molecular structure and conformation of plant-based proteins play a significant role in their gelation behavior. This study compared the rheological and textural properties of gels formed by lentils, mungbean, and yellow pea proteins with those of commercial soy and pea protein isolates. The results showed that mungbean protein had the lowest critical protein concentration and formed stronger gels compared to lentils and yellow pea proteins. Commercial isolates with larger particle size, water holding capacity, and denatured state had lower critical protein concentrations than their folded and more soluble counterparts. These findings provide insights into the structure-gelation relationship of pulse proteins and support the development of plant protein-gelled products beyond meat and dairy analogues.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Multidisciplinary
Rahul Sharma, Kevin Putera, Mark M. Banaszak Holl, Gil Garnier, Victoria S. Haritos
Summary: Fine nanocellulose fibers were obtained through a tandem enzyme reaction using carboxylated cellulose, as an alternative to the conventional toxic chemical process. The combination of fungal laccases and lytic polysaccharide monooxygenases (LPMOs) mimicked nature and resulted in hydrolyzed and oxidized cellulose, leading to the formation of nanofibers.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Christian K. O. Dzuvor, Bhuvana K. Shanbhag, Tayyaba Younas, Hsin-Hui Shen, Victoria S. Haritos, Lizhong He
Summary: Antimicrobial nanomaterials, such as protein-based nanoparticles, have been developed to combat antibiotic-resistant bacteria. In this study, protein-only nanoparticles were engineered to target resistant Gram-positive bacteria. The engineered nanoparticles showed stronger bactericidal ability, enhanced stability, and caused extensive damages to bacterial cell walls, making them a promising alternative to conventional antibiotics.
ACS APPLIED BIO MATERIALS
(2022)
