Article
Biochemistry & Molecular Biology
Sarah Adriana Scuderi, Laura Cucinotta, Alessia Filippone, Marika Lanza, Michela Campolo, Irene Paterniti, Emanuela Esposito
Summary: This study investigates the effect of melatonin on psoriatic phenotype and S. aureus infection-associated psoriasis using an in vitro model. The results demonstrate that melatonin reduces histological damage, restores tight-junction expression, and reduces pro-inflammatory cytokine levels. Melatonin may have potential as a promising strategy for treating psoriasis-like skin inflammation and complications of psoriasis, such as S. aureus infection.
Article
Geriatrics & Gerontology
Julia T. Oliveira, Vanja Dakic, Gabriela Vitoria, Carolina da S. G. Pedrosa, Mayara Mendes, Luiz Guilherme H. S. Aragao, Thyago R. Cardim-Pires, Damien Lelievre, Daniel Rodrigues Furtado, Roberta O. Pinheiro, Debora Foguel, Lionel Breton, Charbel Bouez, Rodrigo De Vecchi, Marilia Zaluar P. Guimaraes, Stevens Rehen
Summary: This study reveals that oligomeric alpha-Synuclein (O alpha-Syn) induces inflammation and degeneration in the skin, leading to a decrease in keratinocyte proliferation and thickness of the epidermis. These findings shed light on the potential role of O alpha-Syn in the aging process of the skin.
NEUROBIOLOGY OF AGING
(2022)
Article
Cell Biology
Jeniffer Farias Dos Santos, Bruna Leticia Freitas-Marchi, Gustavo Roncoli Reigado, Silvia Romano de Assis, Silvya Stuchi Maria Engler, Felipe Santiago Chambergo Alcalde, Viviane Abreu Nunes
Summary: In skin traumas, tissue engineering using mesenchymal stem cells (MSC) from the umbilical cord shows promise for skin regeneration. MSCs can differentiate into keratinocytes and express important epidermal markers, indicating their potential use in producing skin substitutes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Toxicology
Hui Kheng Lim, Christopher Owen Hughes, Michelle Jing Sin Lim, Jia'En Jasmine Li, Moumita Rakshit, Calvin Yeo, Kern Rei Chng, Angela Li, Joanne Sheot Harn Chan, Kee Woei Ng, David Ian Leavesley, Benjamin Paul Chapman Smith
Summary: The micronucleus assay is commonly used to evaluate the genotoxic potential of chemicals, but traditional in vitro cell lines do not accurately represent the physiology, biochemistry, and genomic events in the gut. This study adapted and validated the micronucleus assay for use with 3D reconstructed intestinal microtissues, showing that these microtissues replicate the physiological, biochemical, and genomic responses of the native human small intestine. The study highlights the potential of the reconstructed intestine micronucleus cytome assay as a promising tool for predictive toxicology.
ARCHIVES OF TOXICOLOGY
(2022)
Review
Immunology
Praxedis Martin, Jeremie D. Goldstein, Loic Mermoud, Alejandro Diaz-Barreiro, Gaby Palmer
Summary: IL-1 family cytokines play important roles in skin disorders, and dysregulated signaling may lead to the development of chronic inflammatory diseases. Anti-inflammatory IL-1 family members such as IL-1Ra and IL-36Ra control the biological activity of pro-inflammatory IL-1 family agonists. These cytokines are highly expressed in the epidermis and have therapeutic potential for human inflammatory skin diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Ana Sofia Agonia, Ana Palmeira-de-Oliveira, Catarina Cardoso, Catia Augusto, Christian Pellevoisin, Christelle Videau, Ricardo Jorge Dinis-Oliveira, Rita Palmeira-de-Oliveira
Summary: The use of in vitro human skin permeation tests is valuable for evaluating the quality and equivalence of topical drug products. In this study, the use of reconstructed human epidermis (RhE) as a biological membrane for bioequivalence testing of clotrimazole creams demonstrated its discriminatory power and higher permeability compared to human skin.
Article
Pharmacology & Pharmacy
Dorottya Kocsis, Hichem Kichou, Katalin Dome, Zsofia Varga-Medveczky, Zsolt Revesz, Istvan Antal, Franciska Erdo
Summary: Various ex vivo and in vitro skin models are used in dermatological and cosmetic research, some for drug penetration testing. This study compared the chemical composition of different skin models and established a testing system for drug diffusion and accumulation. The results showed variations in chemical composition among the skin models, with the reconstructed skin having lower water content and higher urea and protein levels. The skin-on-a-chip and confocal Raman microspectroscopy were suitable for testing drug penetration and distribution, while caution is advised when testing the absorption of drugs on obese human skin due to its imbalanced composition.
Article
Biochemistry & Molecular Biology
Zsofia Szilagyi, Zsuzsanna Nemeth, Jozsef Bakos, Gyoergyi Kubinyi, Peter Pal Necz, Erika Szabo, Gyoergy Thuroczy, Rosanna Pinto, Brahim Selmaoui
Summary: In recent years, there has been increasing exposure to radiofrequency (RF) radiation emitted by wireless devices in the human environment, which could be absorbed in the body. This study aimed to investigate the combined effects of 2422 MHz (Wi-Fi) RF exposure and UV radiation on the inflammation process in the skin. The results suggest a slight but not significant increase in cytokine concentrations after prior UV exposure to 2422 MHz Wi-Fi radiation, and no adaptive response phenomenon was detected.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Jee-hyun Hwang, Seungmi Lee, Ho Geon Lee, Dalwoong Choi, Kyung-Min Lim
Summary: The effects of acidic pH on skin need to consider both pH and concentration. Organic and inorganic acids can be irritants to the skin at certain concentrations. Alkaline agents also cause damage to the skin.
Article
Toxicology
Takami Akagi, Tomomi Yamada, Hiromi Miyazaki, Hiroyuki Taguchi, Hidefumi Ikeda, Masakazu Katoh, Simona Mura, Patrick Couvreur, Paninee Chetprayoon, Rawiwan Maniratanachote, Hiroaki Yoshida, Hiroharu Ajiro, Koji Hashimoto, Takao Ashikaga, Hajime Kojima, Mitsuru Akashi
Summary: The aim of this study is to validate a skin irritation test using three-dimensional reconstructed human epidermal skin equivalents prepared by a layer-by-layer method. The validation study showed that the developed skin model has similar characteristics to validated reference methods and native human skin. The results obtained from the study demonstrated the reproducibility and accuracy of the test in distinguishing skin irritants from nonirritants.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Chemistry, Analytical
Manuel Chacon, Natalia Vazquez, Sergio Alonso-Alonso, Mairobi Persinal-Medina, Sara Llames, Marta Pevida, Ignacio Alcalde, Jesus Merayo-Lloves, Alvaro Meana
Summary: The rabbit skin irritation test has been the standard for evaluating chemical irritation potential, but alternative methods are encouraged. Reconstructed human epidermis (RhE) models mimic human epidermis and can be used as an alternative. As the use of RhE models increases, new challenges have emerged, such as the need for non-invasive and marker-free methodologies. Electrochemical impedance spectroscopy (EIS) is a promising method to meet these requirements.
Article
Dermatology
Xiao Ma, Feifei Wang, Bo Wang
Summary: The use of 3D skin models for skin irritation testing of cosmetic products provides a more accurate evaluation of the safety and efficacy of the products. The study found that certain cosmetic products can cause skin irritation based on tissue activity and IL-1 alpha expression, highlighting the importance of using 3D skin models for such evaluations.
JOURNAL OF COSMETIC DERMATOLOGY
(2021)
Article
Toxicology
Hideyuki Mizumachi, Sho Suzuki, Megumi Sakuma, Midori Natsui, Noriyasu Imai, Masaaki Miyazawa
Summary: The hazards and potency of skin sensitizers have traditionally been determined using animal tests, but significant progress has been made in the development of non-animal test methods like EpiSensA, which can accurately predict and detect sensitizing constituents and mixtures with higher sensitivity and applicability.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Irini M. Dijkhoff, Benedetta Petracca, Roxane Prieux, Giuseppe Valacchi, Barbara Rothen-Rutishauser, Marc Eeman
Summary: The three-dimensional human epidermis model reconstructed from neonatal primary keratinocytes mimics the morphology of native human skin, serving as a tool to assess environmental effects and a broad range of skin-related studies. The model displays fully differentiated stratified epidermis and responsive barrier functions to proinflammatory stimuli, making it a straightforward and reproducible method for in vitro studies.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Oncology
Elisabetta Michielon, Marta Lopez Gonzalez, Dorian A. Stolk, Joeke G. C. Stolwijk, Sanne Roffel, Taco Waaijman, Sinead M. Lougheed, Tanja D. de Gruijl, Susan Gibbs
Summary: Invasion, immune modulation, and angiogenesis are crucial in melanoma progression. This study successfully generated several Mel-RhS models mimicking different stages of melanoma progression and demonstrated their potential for studying the disease and evaluating therapeutic strategies.
