Enhanced expression and solubility of main protease (Mpro) of SARS-CoV-2 from E. coli

Repurposing of US-FDA approved drugs against SARS-CoV-2 main protease (Mpro) by using STD-NMR spectroscopy, in silico studies and antiviral assays

(2023) A. Mateen Khan et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Quinazolinone-Peptido-Nitrophenyl-Derivatives as Potential Inhibitors of SARS-CoV-2 Main Protease

(2023) Huynh-Nguyet-Huong Giang et al.   Viruses-Basel

Crystal Structures of Inhibitor-Bound Main Protease from Delta- and Gamma-Coronaviruses

(2023) Sarah N. Zvornicanin et al.   Viruses-Basel

Search of Novel Small Molecule Inhibitors for the Main Protease of SARS-CoV-2

(2023) Wenfa Zhang et al.   Viruses-Basel

Effect of chitooligosaccharide on the inhibition of SARS-CoV-2 main protease

(2023) Qian Wang et al.   Biomaterials Research

Structural Basis for the Inhibition of Coronaviral Main Proteases by a Benzothiazole-Based Inhibitor

(2022) Xiaohui Hu et al.   Viruses-Basel

SARS-CoV-2 Main Protease Drug Design, Assay Development, and Drug Resistance Studies

(2022) Bin Tan et al.   Accounts of Chemical Research

Multiple pathways for SARS-CoV-2 resistance to nirmatrelvir

(2022) Sho Iketani et al.   NATURE

Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor

(2021) Krishna Kumar Haridhasapavalan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structural Basis of Potential Inhibitors Targeting SARS-CoV-2 Main Protease

(2021) Hylemariam Mihiretie Mengist et al.   Frontiers in Chemistry

Scutellaria barbata D. Don Inhibits the Main Proteases (Mpro and TMPRSS2) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection

(2021) Sheng-Teng Huang et al.   Viruses-Basel

The Repurposed Drugs Suramin and Quinacrine Cooperatively Inhibit SARS-CoV-2 3CLpro In Vitro

(2021) Raphael J. Eberle et al.   Viruses-Basel

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

(2021) Nadide Altincekic et al.   Frontiers in Molecular Biosciences

Repurposing clinically approved drugs for COVID-19 treatment targeting SARS-CoV-2 papain-like protease

(2021) Yunxia Xu et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Effects of Lower Temperature on Expression and Biochemical Characteristics of HCV NS3 Antigen Recombinant Protein

(2021) Chen-Ji Huang et al.   Catalysts

The deadly coronaviruses: The 2003 SARS pandemic and the 2020 novel coronavirus epidemic in China

(2020) Yongshi Yang et al.   JOURNAL OF AUTOIMMUNITY

Emerging coronaviruses: Genome structure, replication, and pathogenesis

(2020) Yu Chen et al.   JOURNAL OF MEDICAL VIROLOGY

Drug Development and Medicinal Chemistry Efforts toward SARS‐Coronavirus and Covid‐19 Therapeutics

(2020) Arun K. Ghosh et al.   ChemMedChem

Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease

(2020) Chunlong Ma et al.   CELL RESEARCH

Structural stability of SARS-CoV-2 3CLpro and identification of quercetin as an inhibitor by experimental screening

(2020) Olga Abian et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Low temperature bacterial expression of the neutral amino acid transporters SLC1A5 (ASCT2), and SLC6A19 (B0AT1)

(2020) Michele Galluccio et al.   MOLECULAR BIOLOGY REPORTS

The SARS-CoV-2 main protease as drug target

(2020) Sven Ullrich et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Both Boceprevir and GC376 efficaciously inhibit SARS-CoV-2 by targeting its main protease

(2020) Lifeng Fu et al.   Nature Communications

Feline coronavirus drug inhibits the main protease of SARS-CoV-2 and blocks virus replication

(2020) Wayne Vuong et al.   Nature Communications

Biochemical and biophysical characterization of the main protease, 3-chymotrypsin-like protease (3CLpro) from the novel coronavirus SARS-CoV 2

(2020) Juliana C. Ferreira et al.   Scientific Reports

Recombinant production of active Streptococcus pneumoniae CysE in E. coli facilitated by codon optimized BL21(DE3)-RIL and detergent

(2019) Deepali Verma et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Soluble expression of IGF1 fused to DsbA in SHuffle™ T7 strain: optimization of expression and purification by Box-Behnken design

(2019) Nabbi Emamipour et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A general platform for efficient extracellular expression and purification of Fab from Escherichia coli

(2019) Manyu Luo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression, purification, and characterization of the native intracellular domain of human epidermal growth factor receptors 1 and 2 in Escherichia coli

(2019) Supaphorn Seetaha et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression of a Chimeric Allergen with High Rare Codons Content in Codon Bias-Adjusted Escherichia coli: Escherichia coli BL21 (DE3)-Codon Plus RIL as an Efficient Host

(2016) Hamid Reza Nouri et al.   CURRENT MICROBIOLOGY

Spectroscopic and thermodynamic properties of recombinant heat shock protein A6 from Camelus dromedarius

(2014) Ajamaluddin Malik et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

(2014) Sofia Costa et al.   Frontiers in Microbiology

Recombinant protein expression in Escherichia coli: advances and challenges

(2014) Germán L. Rosano et al.   Frontiers in Microbiology

Mass spectrometry-based protein identification by integrating de novo sequencing with database searching

(2013) Penghao Wang et al.   BMC BIOINFORMATICS

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

(2012) David J. Leibly et al.   PLoS One

Soluble expression and purification of the anthrax protective antigen in E. coli and identification of a novel dominant-negative mutant N435C

(2010) Gaobing Wu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY