Journal
MOLECULES
Volume 21, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/molecules21050560
Keywords
Bailinggu; comparative transcriptomic analysis; cold stress; qPCR-PCR; EST-SSR
Funding
- National Basic Research Program of China [2014CB138305]
- National Natural Science Foundation of China [31471926]
- Natural Science Foundation of Jilin Province [20140101149JC]
- University S AMP
- T Innovation Platform of Jilin Province for Economic Fungi [2014B-1]
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Cold stimulation of Bailinggu's mycelia is the main factor that triggers primordia initiation for successful production of fruiting bodies under commercial cultivation. Yet, the molecular-level mechanisms involved in mycelia response to cold stimulation are still unclear. Here, we performed comparative transcriptomic analysis using RNA-Seq technology to better understand the gene expression regulation during different temporal stages of cold stimulation in Bailinggu. A total of 21,558 Bailinggu mycelia unigenes were de novo assembled and annotated from four libraries (control at 25 degrees C, plus cold stimulation treatments at -3 degrees C for a duration of 1-2 days, 5-6 days, and 9-10 days). GO and KEGG pathway analysis indicated that functional groups of differentially expressed unigenes associated with cell wall and membrane stabilization, calcium signaling and mitogen-activated protein kinases (MAPK) pathways, and soluble sugars and protein biosynthesis and metabolism pathways play a vital role in Bailinggu's response to cold stimulation. Six hundred and seven potential EST-based SSRs loci were identified in these unigenes, and 100 EST-SSR primers were randomly selected for validation. The overall polymorphism rate was 92% by using 10 wild strains of Bailinggu. Therefore, these results can serve as a valuable resource for a better understanding of the molecular mechanisms associated with Bailinggu's response to cold stimulation.
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