Article
Biochemistry & Molecular Biology
Roberto Morellon-Sterling, Juan M. Bolivar, Roberto Fernandez-Lafuente
Summary: The immobilization of ficin on vinyl sulfone agarose caused its almost complete inactivation, possibly due to the modification of catalytic cysteine. By switching off ficin with dipyridyl-disulfide during immobilization and switching it on with 1 M beta-mercaptoethanol, the activity of the immobilized ficin could be significantly increased.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Khadijeh Pourkhanali, Gholam Khayati, Farhang Mizani, Fereshteh Raouf
Summary: This study investigated the immobilization of lipase from Penicillium sp. onto modified bentonites using adsorption and crosslinking methods. The immobilized lipase showed better thermal and pH stability compared to free lipase. Among the immobilized enzymes, GDU-bent-lipase exhibited the most efficient performance in various aspects.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Shailja Mishra, Biswajit Hansda, Ankit Ghosh, Sneha Mondal, Bhabatosh Mandal, Pallavi Kumari, Basudev Das, Tanay Kumar Mondal, Tirtha Biswas
Summary: Enzymes have great potential in industrial applications, but utilizing them at high efficiency levels, especially above room temperature, has not been fully realized. The immobilization of papain onto homo-functionalized silica gel via multipoint covalent attachment has successfully addressed this issue, resulting in a stable and reusable enzyme catalyst with improved activity and adaptability to higher temperatures.
Review
Biochemistry & Molecular Biology
Veymar G. Tacias-Pascacio, Roberto Morellon-Sterling, Daniel Castaneda-Valbuena, Angel Berenguer-Murcia, Majid Rasool Kamli, Olga Tavano, Roberto Fernandez-Lafuente
Summary: This paper reviews the immobilization of papain on different supports for the first time, with a special focus on the preparation of magnetic biocatalysts and specific problems related to the immobilization of proteases. The benefits of proper immobilization and potential artifacts are discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Juan Han, Ting Zhang, Zhuoyue Zhou, Houjin Zhang
Summary: The demand for greener food production has led to a preference for biocatalysts over traditional approaches. The efficiency of biocatalysts is limited by immobilization strategies. This study developed a novel immobilization method using the tyrosine-tag crosslinking mechanism and enhanced immobilization efficiency with ultrasound treatment. The strategy proved effective in immobilizing food enzyme lipase, d-amino acid oxidase, and glucose dehydrogenase on various materials. The developed enzyme immobilization strategy has significant implications for the application of biocatalysts in the food industry.
Article
Engineering, Chemical
Zhe Dong, Jian Tan, Manuel Pinelo, Hao Zhang, Yinhua Wan, Jianquan Luo
Summary: This study developed a mussel-inspired coating for enzyme immobilization. Through the screening of different silane coupling agents and optimization of treatment methods, the coating showed improved enzyme loading, activity, and pH tolerance. The optimized coating exhibited excellent stability and reusability at different pH conditions.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Pedro Abellanas-Perez, Diego Carballares, Roberto Fernandez-Lafuente, Javier Rocha-Martin
Summary: This study immobilized two lipases, CALB and TLL, on octyl agarose, and observed the stability of the enzymes under different treatment methods and buffers. It was found that the stability of the enzymes varied depending on the treatment method and buffer used. The presence of intermolecular crosslinkings enhanced stability for the highly loaded enzymes, while intramolecular crosslinkings played a role in stabilizing the lowly loaded enzymes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Chan Hee Lee, Hye Sun Lee, Jae Won Lee, Jangyong Kim, Jin Hyung Lee, Eon Seon Jin, Ee Taek Hwang
Summary: Enzyme immobilization using inorganic materials, such as CaCO3, has been shown to improve enzyme stability and activity. In this study, in situ and crosslink-immobilized enzymes in a CaCO3 carrier were compared, with both methods preserving over 30% of initial enzyme activity after 180 days. However, differences in enzyme loading, activity, and characteristics were observed between the two methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Multidisciplinary
Nian Shi, Mingqiang Zheng, Xinming Wu, Ning Chen, Luying Jiang, Baogen Chang, Fuping Lu, Fufeng Liu
Summary: A novel method of affinity peptide orientation and light-controlled covalent immobilization was developed in this study. The targeted immobilization region was selected using molecular simulation, and a short peptide with high affinity was designed and validated. Through directional photo-crosslinking, the enzymes were immobilized onto the surface of the epoxy resin, resulting in improved recycling and heat resistance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jose R. Guimaraes, Diego Carballares, Javier Rocha-Martin, Paulo W. Tardioli, Roberto Fernandez-Lafuente
Summary: Mineralization of immobilized enzymes can improve their properties, but the effects depend on the immobilization protocol and enzyme type.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Diego Carballares, Roberto Fernandez-Lafuente, Javier Rocha-Martin
Summary: This paper presents different methodologies for the immobilization of D-amino acid oxidase (DAAO). The results suggest that immobilization on glutaraldehyde support at pH 7 provides the best stability and activity for the enzyme.
PROCESS BIOCHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Qikai Tian, Jing Zhang, Jing Yan, Shaobin Zhang, Ziyi Yu
Summary: Pickering interfacial biocatalysis has gained significant attention due to the need for cleaner and more efficient chemical industry. The crucial step in this process is the preparation of enzyme-loaded particles, which often involves complex modification and functionalization processes. This study presents a one-step method to generate polymer nanoparticles with innate carboxyl functional groups, offering adjustable wettability and excellent reusability and storage stability for the immobilized enzymes.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Hector Sanchez-Moran, James S. Weltz, Daniel K. Schwartz, Joel L. Kaar
Summary: "The long-term goal of biotechnology is to develop design rules for stabilizing enzymes when immobilized to materials. By investigating the stabilization of lipases immobilized on PEGMA and SBMA copolymer brush surfaces, it was found that the stability of the lipases at elevated temperatures depended on the fraction of PEGMA in the brush layer. This understanding can lead to the rational tuning of enzyme-material interfaces for reliable ruggedization of enzymes under extreme conditions."
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Jose M. Guisan, Gloria Fernandez-Lorente, Javier Rocha-Martin, Daniel Moreno-Gamero
Summary: This review discusses different strategies for the preparation of efficient and robust immobilized biocatalysts, including immobilization on pre-existing porous supports, multipoint covalent attachment on support surfaces, and additional stabilization through physical or chemical modification with polymers. These strategies can be easily developed and result in stabilizations ranging between 1000 and 100,000 folds compared to diluted soluble enzymes.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2022)
Article
Agricultural Engineering
Chunyang Bao, Yan Wang, Xiaoling Xu, Die Li, Jing Chen, Zhangbin Guan, Bingyu Wang, Mei Hong, Jingyu Zhang, Tianheng Wang, Qiang Zhang
Summary: A facile strategy was developed for the reversible immobilization of enzymes by utilizing non-covalent interaction between protein and carbohydrate. Laccase was successfully immobilized onto glycopolymer microspheres with high enzyme loading and yield, showing enhanced pH stability and activity in biodegradation. The immobilized laccases could be recycled multiple times while the glycopolymer microspheres could be efficiently regenerated for further enzyme immobilization, making them potential reusable carriers for different enzymes.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Applied
Rodolpho R. C. Monteiro, Jose J. Virgen-Ortiz, Angel Berenguer-Murcia, Thays N. da Rocha, Jose C. S. dos Santos, Andres R. Alcantara, Roberto Fernandez-Lafuente
Summary: This review presents some of the latest studies on lipase A from Candida antarctica (CALA), focusing on its stability and ability to attack the sn-2 position of triglycerides, as well as its commercial availability. It discusses the cloning, production, structural facts, and applications of the enzyme, with a special emphasis on enzyme immobilization.
Article
Biotechnology & Applied Microbiology
S. Fabbricino, S. Del Prete, M. E. Russo, C. Capasso, A. Marzocchella, P. Salatino
Summary: The study focuses on using a thermostable CA-based biocatalyst for CO2 absorption, with results showing the positive impact of reusability and equilibration with alkaline buffer on the performance of the catalyst. Further studies on optimizing enzyme loading and handling in CO2 absorption reactors are encouraged.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Polymer Science
Laura Celina Ochoa-Meza, Eber Addi Quintana-Obregon, Irasema Vargas-Arispuro, Alejandro Bernardo Falcon-Rodriguez, Emmanuel Aispuro-Hernandez, Jose J. Virgen-Ortiz, Miguel Angel Martinez-Tellez
Summary: Strategies to combat wheat diseases include global monitoring of pathogens, developing resistant genetic varieties, and applying agrochemicals, but their suitability is not permanent, leading to exploration of alternative options based on stimulating plants' systemic responses.
Review
Chemistry, Physical
Giuseppe Olivieri, Rene H. Wijffels, Antonio Marzocchella, Maria Elena Russo
Summary: This review focuses on the development of enzymatic saccharification technologies in industrial processes, aiming to maximize sugar yields and concentrations, while minimizing the impact of enzyme costs. Discussions include bioreactor configurations, operating modes, and the application of laccase in lignin depolymerization.
Article
Chemistry, Physical
Veymar G. Tacias-Pascacio, Arnulfo Rosales-Quintero, Rafael C. Rodrigues, Daniel Castaneda-Valbuena, Pablo F. Diaz-Suarez, Beatriz Torrestiana-Sanchez, Erik F. Jimenez-Gomez, Roberto Fernandez-Lafuente
Summary: This study evaluated the aqueous enzymatic extraction method for obtaining oil from mamey sapote seed, finding that the oil obtained by AEE had higher oxidative stability compared to conventional solvent extraction. The optimal conditions for AEE were determined, leading to a higher quality oil with enhanced oxidative stability.
Article
Energy & Fuels
F. Raganati, A. Procentese, G. Olivieri, M. E. Russo, P. Salatino, A. Marzocchella
Summary: This study demonstrates the intensive production of butanol using immobilized cells of Clostridium acetobutylicum in a novel continuous fermentation system. By implementing a stagewise simulated moving bed design and integrating the reaction and the product recovery sections, the system achieves high productivity and concentration of butanol.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Agricultural Engineering
Pablo Diaz-Suarez, Arnulfo Rosales-Quintero, Roberto Fernandez-Lafuente, Enrique Pola-Sanchez, Maritza C. Hernandez-Cruz, Sandy L. Ovando-Chacon, Rafael C. Rodrigues, Veymar G. Tacias-Pascacio
Summary: This study successfully extracted oil from castor seeds using aqueous enzymatic extraction, achieving a high oil yield of 80% under optimized conditions. Compared with solvent extraction, oil extracted using AEE exhibited higher oxidative stability, indicating higher quality.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Food Science & Technology
Karen Chavez-Zaragoza, Alejandro Morales-Guerrero, Citlali Colin-Chavez, Luis Tovar-Diaz, Jose de Jesus Ornelas-Paz, Juan A. Osuna-Castro, Irasema Vargas-Arispuro, Miguel A. Martinez-Tellez, Jose J. Virgen-Ortiz
Summary: Blue LED treatment delays the senescence processes in mangoes, improves their antioxidant capacity and nutritional status, and enhances the activity of antioxidant enzymes.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Maria Elena Russo, Clemente Capasso, Antonio Marzocchella, Piero Salatino
Summary: Carbonic anhydrase (CA) is a promising biocatalyst for the capture and utilization of CO2. Efficient immobilization methods have made it increasingly attractive for continuous bioreactors and industrial applications. This mini-review discusses various CA immobilization techniques and their advantages and disadvantages in CO2 capture and utilization processes. It also highlights the criteria for selecting the optimal CA-biocatalyst and designing CO2 absorption units, as well as presents potential applications of CA in enzymatic CO2 absorption processes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
M. E. Russo, A. Procentese, F. Montagnaro, A. Marzocchella
Summary: The use of waste lignocellulose feedstock for sugar-based biorefineries is of interest to scientists and industries. This study characterized the enzymatic hydrolysis kinetics of coffee silverskin and provided an optimized process that can be applied to various biomasses.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Food Science & Technology
Irma Ofelia Maya-Meraz, Jose de Jesus Ornelas-Paz, Jaime David Perez-Martinez, Alfonso A. Gardea-Bejar, Claudio Rios-Velasco, Saul Ruiz-Cruz, Juan Ornelas-Paz, Ramona Perez-Leal, Jose Juan Virgen-Ortiz
Summary: This study found that spraying 'Shiraz' vines with CaCO3-rich residues from the cement industry can increase fruit yield, improve color development, and enhance the content of phenolic compounds and antioxidant capacity in grapes and wine.
Article
Food Science & Technology
Citlali Colin-Chavez, Jose J. Virgen-Ortiz, Marco A. Miranda-Ackerman, Orlando Hernandez-Cristobal, Miguel Angel Martinez-Tellez, Fabiola Esquivel-Chavez, Nancy Lissete Gallegos-Santoyo
Summary: Active sachets containing oregano oil microencapsulated with starch/agave fructans were developed to control phytopathogens in avocados and reduce postharvest decay. The sachets inhibited the growth of multiple phytopathogens and successfully reduced anthracnose infection in avocados without negative effects on fruit quality. In addition, the sachets increased the activity of antioxidant enzymes and the contents of phenolic compounds in treated avocados.
Article
Biotechnology & Applied Microbiology
G. Ruggiero, F. Lanzillo, F. Raganati, M. E. Russo, P. Salatino, A. Marzocchella
Summary: A dynamic-kinetic model for gas fermentation of C. carboxidivorans was developed and validated in this study. The model considered gas-liquid mass transfer, carbon monoxide uptake, and multiple conversion pathways of acid/alcohol production. The analysis revealed that adjusting the operating conditions could greatly improve the fermentation performance.
FOOD AND BIOPRODUCTS PROCESSING
(2022)
Article
Food Science & Technology
Fabiola Esquivel-Chavez, Citlali Colin-Chavez, Jose Juan Virgen-Ortiz, Miguel Angel Martinez-Tellez, Roberto de Jesus Avena-Bustillos, Gabriela Pena-Madrigal, Marco A. Miranda-Ackerman
Summary: The aim of this study was to control phytopathogens associated with mango decay by exposing them to antifungal thyme oil-starch/agave fructans microcapsules in Nylon sachets. Results showed that different doses of the microcapsules effectively inhibited the growth of pathogens and reduced the incidence and severity of Colletotrichum gloeosporioides in mango.
