4.6 Article

Bryonolic Acid, a Triterpenoid, Protect Against N-methyl-D-Aspartate-Induced Neurotoxicity in PC12 Cells

Journal

MOLECULES
Volume 21, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/molecules21040418

Keywords

bryonolic acid; PC12 cells; Ca2+; Bcl-2; Bax; p-CaMKII; p-CREB

Funding

  1. Important Subject of Fujian Province Science and Technology Hall of China [2012Y0041]
  2. National College Students' Innovative Entrepreneurial Training Plan Project [201410393008]
  3. Strategic Emerging Industries Project of Fujian Province [2015Y0067]

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Calciumoverload is considered to be one of themechanisms of cerebral ischemia. Ca2+ influx and Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cAMP response element-binding protein (CREB) phosphorylation are considered to be involved in N-Methyl-D-aspartate (NMDA)-induced apoptosis process. This study investigated the neuroprotective effects of bryonolic acid (BA) in an NMDA-induced rat adrenal pheochromocytoma cell line (PC12) cells and the potential mechanism. PC12 was treated by NMDA to establish an excitotoxicity model. BA (110,100 and 1000 mu M final concentration) was added to the medium 24 h prior to the addition of NMDA. Subsequently, a methyl thiazolyl tetrazolium (MTT) assay and a lactate dehydrogenase (LDH) release were performed. Ca2+ concentration was demonstrated using a scanning-dual wavelength fluorimetric method. In addition, protein and mRNA levels were determined via Western blot and real-time PCR. In the presence of BA, MTT assay and LDH assay showed that more cells were viable in comparison with the NMDA group. Moreover, the concentration of Ca2+ decreased with the addition of BA in culture. Furthermore, BA could upregulate protein expressions of Bcl-2, p-CREB, and p-CaMKII and downregulate protein expression of Bax. The mRNA results showed that the pattern of mRNA expression were similar to their respective protein levels. All these results indicate that BA protected PC12 cells against NMDA-induced apoptosis by inhibiting Ca2+ influx and regulating gene expression in the Ca2+-CaMKII-CREB signal pathway. Therefore, the present study supports the notion that BA may be a promising neuroprotective agent for the treatment of cerebral ischemia disease.

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