Article
Chemistry, Analytical
Hao Chang, Yu Mei, Yidan Li, Luqing Shang
Summary: In this study, a novel neuraminidase fluorescent probe with aggregation-induced emission and excited state intramolecular proton transfer activity was developed for influenza virus detection. The probe showed high sensitivity and strong fluorescence signal towards neuraminidase, making it suitable for influenza virus detection and living cell imaging. It was also able to distinguish oseltamivir-resistant influenza virus from wild type.
Article
Chemistry, Medicinal
Hongqian Zhao, Siyuan Jiang, Zhifan Ye, Hongxi Zhu, Baichun Hu, Peipei Meng, Yanmei Hu, Huicong Zhang, Kuanglei Wang, Jun Wang, Yongshou Tian
Summary: The study designed a series of oseltamivir derivatives bearing hydrazide fragment, successfully targeting the 150 cavity, showing potent activity and satisfactory metabolic stability. This provides an important reference for potent inhibition against the H274Y mutant of NAs.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Wenjuan Du, Erik de Vries, Frank J. M. van Kuppeveld, Mikhail Matrosovich, Cornelis A. M. de Haan
Summary: Human influenza A viruses originate from animal viruses, with HA and NA playing important roles in the virus particles, although the role of NA in host adaptation is less understood. NA plays a crucial role in adjusting the HA-NA balance of virus particles and affecting virus replication, likely influencing the viral host range.
Article
Biochemistry & Molecular Biology
Md Amran Howlader, Ekaterina P. Demina, Suzanne Samarani, Tianlin Guo, Antoine Caillon, Ali Ahmad, Alexey Pshezhetsky, Christopher W. Cairo
Summary: The isoenzymes of the neuraminidase family (NEU1, 3, and 4) play essential roles in the activation, extravasation, and migration of immune cells in the induction of acute inflammatory response. NEU1 and NEU3 deficiency decreases leukocyte recruitment, while NEU4 deficiency increases it, indicating opposite effects of NEU isoenzymes on leukocyte infiltration. Further investigation is needed to determine the mechanisms and importance of NEU isoenzymes as regulators of the inflammatory cascade.
Article
Immunology
Nelson Lee, Stephanie W. Smith, David S. C. Hui, Ming Ye, Nathan Zelyas, Paul K. S. Chan, Steven J. Drews, Lori Zapernick, Rity Wong, Mary Labib, Sandy Shokoples, Dean T. Eurich
Summary: The study proposed a 6-point ordinal scale for studying outcomes of adults hospitalized with influenza, and found that treatment with neuraminidase inhibitors could improve clinical status over 30 days. Baseline scoring and age of patients were found to have an impact on the outcomes.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Chemistry, Medicinal
Mengwu Xiao, Lvjie Xu, Ding Lin, Wenwen Lian, Manying Cui, Meng Zhang, Xiaowei Yan, Shuishi Li, Jun Zhao, Jiao Ye, Ailin Liu, Aixi Hu
Summary: A series of 4-thiazolinone derivatives were synthesized and evaluated for their inhibitory activities on neuraminidase from various influenza virus subtypes. The study identified several compounds with potent inhibitory activities against different influenza viral subtypes, indicating their potential as candidates for new anti-influenza drug development.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Liming Yu, Jun Peng, Chieko Mineo
Summary: Sialylation is a common modification in eukaryotic cells that plays a key role in various biological processes. It regulates protein-protein interactions, intracellular localization, vesicular trafficking, and signal transduction. Sialylation of lipoproteins is closely related to lipoprotein metabolism and atherogenesis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Chika Kikuchi, Aristotelis Antonopoulos, Shengyang Wang, Tadashi Maemura, Rositsa Karamanska, Chiara Lee, Andrew J. Thompson, Anne Dell, Yoshihiro Kawaoka, Stuart M. Haslam, James C. Paulson
Summary: The evolution of human H3N2 influenza viruses has led to a limited specificity of the hemagglutinin to a subset of glycan receptors, presenting challenges for virus recognition and vaccine production. By studying glyco-engineered cell lines, the authors demonstrate the importance of extended glycan receptors for the growth of recent H3N2 viruses and their relevance to vaccine production.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Asuka Shirakawa, Yoshiyuki Manabe, Roberta Marchetti, Kumpei Yano, Seiji Masui, Alba Silipo, Antonio Molinaro, Koichi Fukase
Summary: The fully sialylated tetraantennary N-glycan was synthesized for the first time using a specific strategy, and the synthesized compounds were used to study the recognition mechanism of neuraminidase.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Pharmacology & Pharmacy
Sook Kwan Brown, Yeu-Yang Tseng, Ammar Aziz, Mariana Baz, Ian G. Barr
Summary: This study tested 3425 influenza B viruses collected from the Asia-Pacific region against four registered neuraminidase inhibitors (NAIs). It identified 45 influenza B viruses with reduced susceptibility to one or more NAIs, including some with newly identified neuraminidase mutations. The study highlights the potential impact of these mutations on the clinical effectiveness of NAIs in treating influenza B infections in humans.
ANTIVIRAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Harrison Steele, Andrew J. Tague, Danielle Skropeta
Summary: Respiratory infections caused by viruses like influenza and coronavirus pose a serious global problem with high infection rates and pandemic potential. Vaccination is the most successful prevention strategy, but not always available. Hence, there is a need for broad-spectrum anti-viral drugs to treat such infections, especially for immunocompromised patients or outbreaks of novel virus strains.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Anil Gattani, Aditya Agrawal, M. Hira Khan, Rohini Gupta, Praveen Singh
Summary: With the exception of plants, nearly all organisms produce neuraminidase/sialidase, which plays a crucial role in determining the virulence of microorganisms. Neuraminidase, an important enzyme in orthomyxoviruses and paramyxoviruses, acts by destroying receptors. In addition to facilitating the release of viral particles, it also performs various other functions throughout the viral life cycle. Neuraminidase is a valuable target for therapeutic and diagnostic purposes, with inhibitors effectively preventing disease spread and viral infection. While traditional assays are commonly used to characterize neuraminidase activity, there is a need for sensitive, rapid, and cost-effective high throughput assays, including those involving biosensors.
ANALYTICAL BIOCHEMISTRY
(2023)
Review
Chemistry, Applied
Alvin Jin Wei Ling, Lee Sin Chang, Abdul Salam Babji, Jalifah Latip, Mamoru Koketsu, Seng Joe Lim
Summary: Sialic acids, a group of alpha-keto acids with nine carbons, exist in over 50 forms, with sources ranging from edible bird's nests to enzymatic extraction. Recognized for its bioactivities, sialic acid can be utilized as a functional ingredient across various industries.
Article
Immunology
Konstantin Andreev, Jeremy C. Jones, Patrick Seiler, Ahmed Kandeil, Jasmine C. M. Turner, Subrata Barman, Adam M. Rubrum, Richard J. Webby, Elena A. Govorkova
Summary: The antiviral susceptibility of currently circulating highly pathogenic avian influenza A(H5N1) viruses was assessed using genotypic and phenotypic approaches. The majority of the viruses retain susceptibility to antiviral drugs, but novel mutations have been identified that reduce the inhibitory effects of certain drugs.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Ruifang Jia, Jiwei Zhang, Jian Zhang, Chiara Bertagnin, Anna Bonomini, Laura Guizzo, Zhen Gao, Xiangkai Ji, Zhuo Li, Chuanfeng Liu, Han Ju, Xiuli Ma, Arianna Loregian, Bing Huang, Peng Zhan, Xinyong Liu
Summary: Novel boron-containing derivatives were designed and synthesized to address drug resistance to influenza virus. Compound 2c exhibited strong antiviral activity against multiple influenza viruses, low cytotoxicity, and no acute toxicity.
Article
Biochemistry & Molecular Biology
Yuan-Yuan Han, Hai-Yun Yue, Xiao-Yang Zhang, Yong-Mei Lyu, Li Liu, Josef Voglmeir
Summary: This study describes the recombinant expression of an unstudied, heterodimeric beta-galactosidase from Lactobacillus brevis in Escherichia coli, and investigated six different constructs with fused protein subunits. The co-expression of independent and fused subunits in a single Duet vector increased the beta-galactosidase activity significantly. The recombinant beta-galactosidase showed comparable hydrolyzing properties towards different substrates.
PROTEIN AND PEPTIDE LETTERS
(2021)
Article
Chemistry, Analytical
Gerard Comamala, Camilla C. Krogh, Vibe S. Nielsen, Joerg P. Kutter, Josef Voglmeir, Kasper D. Rand
Summary: This study presents an improved method for HDX-MS analysis of challenging PTM-rich proteins by immobilizing enzymes on microfluidic chips to create IMERs. The combination of different enzymes allows for efficient online disulfide-bond reduction, deglycosylation, and proteolysis in HDX-MS analysis. This integrated workflow enables the mapping of epitopes and identification of regions involved in protein interactions.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Huimin Mao, Shurui Li, Binru Yin, Xisha Lin, Jingyu Guo, Ting Wang, Josef Voglmeir, Li Liu
Summary: This study demonstrates that intact human milk N-glycome is transferred into bacterial cells and decomposed intracellularly. The principal components responsible for the probiotic function of human milk N-glycome towards B. infantis ATCC 15697 are galactose-containing glycans.
Article
Food Science & Technology
Xisha Lin, Hongliang Yao, Jingyu Guo, Yingying Huang, Wenjiao Wang, Binru Yin, Xiang'an Li, Ting Wang, Chunbao Li, Xinglian Xu, Guanghong Zhou, Josef Voglmeir, Li Liu
Summary: This study investigates the impact of exposure to Neu5Gc-rich red meat on healthy Cmah knock-out mice and the underlying mechanisms. The results indicate that long-term intake of Neu5Gc-rich red meat has no adverse effects on the health of the mice, which may be related to the limited absorption of Neu5Gc regulated by protein glycosylation and the metabolism of Neu5Gc by gut microorganisms.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Cheng Cheng, Zi-Xuan Hu, Meng He, Li Liu, Josef Voglmeir
Summary: This study reports the soluble and functional expression of the human NPL gene in Escherichia coli, and investigates the biochemical properties of two clinically relevant NLP mutations. One mutant variant is enzymatically active but has lower expression levels and reduced stability, while the other mutant variant does not express and therefore has no enzymatic activity.
CARBOHYDRATE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ting Wang, Li Liu, Josef Voglmeir
Summary: Monoclonal antibodies have been successful in treating diseases, with recent research focused on the functional role and production methods of antibody N-glycosylation.
CARBOHYDRATE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Zi-Xuan Hu, Cheng Cheng, Yu-Qian Li, Xiao-Han Qi, Ting Wang, Li Liu, Josef Voglmeir
Summary: This study describes a sialic acid aldolase (sNPL) from a freshwater snail, which shows significant differences in function and substrate scope compared to a sialic acid aldolase from chicken (chNPL). sNPL is active towards sialic acid derivatives but unable to catalyze a specific reaction. Furthermore, sNPL and chNPL exhibit contrasting substrate selectivity, and sNPL has the ability to synthesize a series of 4-hydroxy-2-oxoates, which is not achievable by chNPL.
Article
Plant Sciences
Dusan Velickovic, Yen-Chen Liao, Stephanie Thibert, Marija Velickovic, Christopher Anderton, Josef Voglmeir, Gary Stacey, Mowei Zhou
Summary: In this study, an enzyme-assisted matrix-assisted laser desorption/ionization mass spectrometry imaging strategy was used to visualize the distribution and identity of N-glycans in soybean root nodules. Ion images showed that N-glycosylation mainly occurred in the sclerenchyma layer and infected cells within the infection zone, and mutant nodules had more abundant N-glycan structures. Proteomic analysis revealed that these glycans likely played a role in nitrogen fixation and phenylpropanoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Marius Gramlich, Sandra Maier, Philipp D. Kaiser, Bjoern Traenkle, Teresa R. Wagner, Josef Voglmeir, Dieter Stoll, Ulrich Rothbauer, Anne Zeck
Summary: N-linked glycosylation is a common posttranslational modification of proteins, but it poses challenges for their analytical characterization. A new peptide N-glycanase has been developed that can efficiently work under challenging conditions and has been successfully applied in HDX-MS workflow.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Rui-Rui Guo, Tian-Chan Zhang, Thomas Ole Tandrup Lambert, Ting Wang, Josef Voglmeir, Kasper D. Rand, Li Liu
Summary: This article describes a previously unstudied superacidic bacterial N-glycanase (PNGase H+) from the soil bacterium Rudaea cellulosilytica (Rc), which has significantly improved enzymatic properties compared to previously described variants. PNGase Rc shows faster and more versatile deglycosylation of glycoproteins, making it suitable for N-glycan analysis in different samples.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Plant Sciences
Jianjun Zhang, Yang Xia, Dinghe Wang, Yamin Du, Yongwu Chen, Congcong Zhang, Juan Mao, Muyang Wang, Yi-Min She, Xinxiang Peng, Li Liu, Josef Voglmeir, Zuhua He, Linchuan Liu, Jianming Li
Summary: This study demonstrates that in rescuing bri1-5 protein degradation, AtEDEM1 plays a predominant role over AtEDEM2, while a mutation in ALG3 eliminates the inhibitory effect of irb1-1 on bri1-5 ERAD.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mattia Ghirardello, Yao-Yao Zhang, Josef Voglmeir, M. Carmen Galan
Summary: The use of ionic liquid based derivatisation agents in glycoscience, especially in sensitive mass spectrometry applications, is of great importance. They provide enhanced polarity and labelability to glycans, allowing for the monitoring and detection of glycans in complex samples. Additionally, ionic liquid based derivatisation agents can be used for the labelling of live cells, enabling the detection of cancer biomarkers and tuning of cell surface properties.
CARBOHYDRATE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Lucy I. Crouch, Paulina A. Urbanowicz, Arnaud Basle, Zhi-Peng Cai, Li Liu, Josef Voglmeir, Javier M. Melo Diaz, Samuel T. Benedict, Daniel I. R. Spencer, David N. Bolam
Summary: The human colonic microbiota can utilize complex glycans as a source of nutrients. Gut microbes have a diverse array of carbohydrate-active enzymes that can degrade plant N-glycans. The discovery of these enzymes provides a potential tool for modifying plant N-glycans for various applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Jin-Min Shi, Ting-Ting Wu, Hui Zhou, Yao-Yao Zhang, Li Liu, Goran Widmalm, Josef Voglmeir
Summary: Galactokinases (GalKs) are a group of structurally conserved proteins found in all life forms. A unique GalK orthologue from the bacterium Solitalea canadensis (ScGalK) has been discovered with relaxed substrate requirements and the ability to convert xylose to xylose-1-phosphate. Further investigation identified a putative Glucose-1-phosphate Uridyltransferase (ScGPUT) in close proximity to the ScGalK gene, which can convert xylose-1-phosphate to uridine diphosphate xylose (UDP-xylose) with the presence of uridine triphosphate (UTP).
Article
Chemistry, Multidisciplinary
Yao-Yao Zhang, Mattia Ghirardello, Ting Wang, Ai-Min Lu, Li Liu, Josef Voglmeir, M. Carmen Galan
Summary: The novel imidazolium derivative (GITag) enhances ionisation efficiency for improved mass spectrometric detection of oligosaccharides and N-glycans. Direct labelling of human serum samples with GITag on a MALDI target plate eliminates the need for sample pretreatment or clean-up steps.
CHEMICAL COMMUNICATIONS
(2021)