Article
Engineering, Chemical
Yuichi Kato, Takahiro Morimoto, Kazufumi Kobashi, Tetsuji Yamaguchi, Tetsuya Mori, Takushi Sugino, Toshiya Okazaki
Summary: In this study, we investigated the sedimentation of porous nylon-6 particles with and without a density gradient. The analysis of inner fluid buoyancy was used to estimate the size and particle porosity in the liquid phase.
Article
Agriculture, Dairy & Animal Science
Maria V. O. Santos, Andreia M. Silva, Leonardo V. C. Aquino, Lhara R. M. Oliveira, Samara S. J. Moreira, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira
Summary: This study compared three commonly used methods, swim-up, Percoll gradient, and washing by centrifugation, for extracting sperm from collared peccaries. The results showed that swim-up method had poor efficiency in isolating motile sperm, while Percoll gradient and washing by centrifugation had similar quality and fertilization capacity of the sperm. Therefore, Percoll gradient and washing by centrifugation are recommended for sperm extraction in collared peccaries.
Article
Virology
Mara Elena Heinrichs, Daniele De Corte, Bert Engelen, Donald Pan
Summary: Flow cytometry has been used to understand the impact of viruses on shaping marine microbial communities and biogeochemical cycles in the pelagic zone. A new protocol has been developed for the enumeration of marine sediment viruses by flow cytometry, enabling fast and accurate quantification of viral abundances and opening up options for virus sorting, targeted viromics, and single-virus sequencing. Further optimization may be needed for sediments with low biomass or high organic content.
Article
Environmental Sciences
Mingdong Ren, Min Zhang, Fan Fan, Jinsheng Yang, Zhen Yang, Kaining Chen, Yun Chuang Li, Xiaoli Shi
Summary: The study developed a method to effectively separate benthic cyanobacteria in lake sediment and found significant spatial-temporal variations in cyanobacterial biomass in both the water column and sediment. Cyanobacterial biomass in the water column showed peaks in February and April, while sediment biomass showed peaks from October to February.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Yi-Yang Zhan, Daisuke Ogawa, Koki Sano, Xiang Wang, Fumito Araoka, Nobuyuki Sakai, Takayoshi Sasaki, Yasuhiro Ishida
Summary: The article presents a reconfigurable photonic crystal that exhibits single-peak and double-peak structural colors and allows for reversible state transitions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biotechnology & Applied Microbiology
Sofie Van Dorpe, Lien Lippens, Robin Boiy, Claudio Pinheiro, Glenn Vergauwen, Pekka Rappu, Ilkka Miinalainen, Philippe Tummers, Hannelore Denys, Olivier De Wever, An Hendrix
Summary: In this study, an automated liquid handling workstation was developed for the density-based separation of extracellular vesicles (EV) from human body fluids. The performance of the automated method was compared to manual handling by experienced or inexperienced researchers. The results showed that the automated EV separation method significantly reduced the variability in EV recovery and exhibited high reproducibility, specificity, and reduced hands-on time.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ludmila Vcelarova, Vladimir Skalicky, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Ales Pencik, Ondrej Novak
Summary: The endoplasmic reticulum (ER) is a crucial intracellular membrane network involved in protein and lipid synthesis, as well as regulating auxin homeostasis in plants. Isolating ER poses a challenge due to its similarity with other cellular membranes, but an optimized protocol can yield high-quality samples for analyzing auxin metabolites. Analysis of auxin metabolites revealed elevated levels of active auxin form (IAA) in the ER compared to whole plants, and samples prepared using the optim
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Guilan Di, Guorong Zhu, Xinhua Chen, Xiulian Miao, Min Li, Jingqiang Fu, Ning Wang, Lei Zhu, Miaoqin Huang, Caihuan Ke
Summary: The study investigated the phagocytic activities of hyalinocytes and granulocytes in Babylonia areolata using flow cytometry, electron microscopy, and proteomics analysis. Significant differences in phagocytic abilities between the two cell types were observed based on changes in morphological, functional, and molecular features.
Article
Biochemistry & Molecular Biology
Yuki Itoh, Shousaku Itoh, Haruna Naruse, Takumi Kagioka, Mai Thi Hue, Makoto Abe, Mikako Hayashi
Summary: This study found that intracellular density increases during differentiation of osteoblast lineage cells, and this density variation is associated with the differentiation stages. The high-density fraction showed elevated expression of osteoblast markers while the low-density fraction exhibited higher expression of MSC surface markers. This suggests that intracellular density may serve as a novel indicator for osteoblast differentiation stages and density gradient centrifugation is a valuable technique for studying this process.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Angela Ecija-Arenas, Vanesa Roman-Pizarro, Juan Manuel Fernandez-Romero
Summary: A method for monitoring the efficiency of separating hybrid magnetoliposomes using multiphase density gradient centrifugation coupled with a continuous flow system is described. Different h-MLs suspensions containing hydrophobic magnetic gold nanoparticles and various fluorophores were synthesized and separated using the MDGC system. The separation efficiency was tested and different fractions were identified for each separated h-MLs sample.
Article
Multidisciplinary Sciences
Li-fang Guo, Liu Wang, Sai Ren, Ning Su, Kun Wei, Xian-Ge Sun, Xiao-Dong Ren, Qing Huang
Summary: A novel 3D printing leukocyte sorting accumulator combined with centrifugation was demonstrated to achieve label-free initial leukocyte enrichment based on cell density and size. The upgraded design, leukocyte sorting accumulator-3, optimized the recovery of buffy coats compared to earlier models, with mean leukocyte recovery rates of 96.2 +/- 2.38% by pushing method and 94.4 +/- 0.8% by pulling method. New procedures for preliminary leukocyte enrichment are essential for modern clinical and basic research assays.
Article
Chemistry, Applied
Ziyi Wang, Qinghua Liu, Liang Wang, Robert G. Gilbert, Mitchell A. Sullivan
Summary: Liver glycogen, a branched glucose polymer in animals, plays a role as a blood-sugar buffer. This study aims to optimize sucrose density gradient centrifugation for preserving glycogen's molecular structure by varying sucrose solution density and testing a boiling denaturation step. Lower sucrose concentrations and the boiling step were found beneficial in obtaining a more structurally representative and preserved glycogen sample.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemical Research Methods
Jiayang Jiang, Xiaoman Zhou, Chunliu Gao, Rongqin Ke, Qiwei Guo
Summary: In this study, two modified methods for isolating mouse Leydig cells were developed and compared with a regular method. Modified method 1 achieved higher purity and yields than the regular method, while modified method 2, although lower in purity, could still be used before further purification. These alternative methods provide useful options for isolating Leydig cells in mice.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Biology
Usha Punjabi, Ella Roelant, Kris Peeters, Ilse Goovaerts, Helga Van Mulders, Diane De Neubourg
Summary: There is significant variability in semen and sperm DNA fragmentation parameters in a longitudinal IUI trial. Older males tend to have higher SDF values. After semen processing, different patient categories react differently. SDF testing after processing may be crucial for therapeutic purposes.
Article
Biology
Cagla Guler, Sureyya Melil, Umit Ozekici, Yaprak Donmez Cakil, Belgin Selam, Mehmet Cincik
Summary: This study compared the effects of density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in patients with astheno-teratozoospermia. The results showed that the microfluidic chip group had higher sperm motility and a significantly higher proportion of excellent blastocysts compared to the density gradient group.
Article
Engineering, Environmental
Xinping Zhang, Yuxin Guo, Xiaoyang Liu, Shun-Yu Wu, Ya-Xuan Zhu, Shao-Zhe Wang, Qiu-Yi Duan, Ke-Fei Xu, Zi-Heng Li, Xiao-Yu Zhu, Guang-Yu Pan, Fu-Gen Wu
Summary: This study develops a nanotrigger HCFT for simultaneous photodynamic therapy and light-triggered ferroptosis therapy. The nanotrigger can relieve tumor hypoxia, induce enhanced photodynamic reaction, and facilitate the continuation of Fenton reaction, ultimately leading to lethal ferroptosis in tumor cells.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Olumide Bolarinwa Ayodele, Toyin Daniel Shittu, Olayinka S. Togunwa, Dan Yu, Zhen-Yu Tian
Summary: This study focused on the semihydrogenation of acetylene in an ethylene-rich stream using two alloyed Pt catalysts PtCu and PtCo. The PtCu catalyst showed higher activity and ethylene yield compared to PtCo due to its higher unoccupied Pt d-orbital density. This indicates that alloying Pt with Cu is more promising for industrial relevant SHA catalyst.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Guowei Chen, Wen-Cheng Chen, Yaozu Su, Ruicheng Wang, Jia-Ming Jin, Hui Liang, Bingxue Tan, Dehua Hu, Shaomin Ji, Hao-Li Zhang, Yanping Huo, Yuguang Ma
Summary: This study proposes an intramolecular dual-locking design for organic luminescent materials, achieving high luminescence efficiency and performance for deep-blue organic light-emitting diodes. The material also exhibits unique mechanochromic luminescence behavior and strong fatigue resistance.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Joren van Stee, Gregory Hermans, Jinu Joseph John, Koen Binnemans, Tom Van Gerven
Summary: This work presents a continuous solvent extraction method for the separation of cobalt and nickel in a millifluidic system using Cyphos IL 101 (C101) as the extractant. The optimal conditions for extraction performance and solvent properties were determined by investigating the effects of channel length, flow rate, and temperature. The performance of a developed manifold structure was compared to a single-channel system, and excellent separation results were achieved. The continuous separation process using the manifold structure resulted in high purity cobalt and nickel products.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Yan Xu, Jingai Jiang, Xinyi Lv, Hui Li, Dongliang Yang, Wenjun Wang, Yanling Hu, Longcai Liu, Xiaochen Dong, Yu Cai
Summary: A programmed gas release nanoparticle was developed to address the challenges in treating diabetic infected wounds. It effectively removes drug-resistant pathogens and remodels the wound microenvironment using NO and H2S. The nanoparticle can eliminate bacteria and promote wound healing through antibacterial and anti-inflammatory effects.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Tong Xia, Zhilin Xi, Lianquan Suo, Chen Wang
Summary: This study investigated a highly efficient coal dust suppressant with low initial viscosity and high adhesion-solidification properties. The results demonstrated that the dust suppressant formed a network of multiple hydrogen bonding cross-linking and achieved effective adhesion and solidification of coal dust through various chemical reactions.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Jinzhi Cai, Zhenshan Li
Summary: A density functional theory-based rate equation was developed to predict the gas-solid reaction kinetics of CaO carbonation with CO2 in calcium looping. The negative activation energy of CaO carbonation close to equilibrium was accurately predicted through experimental validation.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Jianxiong Chen, Fuhao Ren, Ningning Yin, Jie Mao
Summary: This study presents an economically efficient and easily implementable surface modification approach to enhance the high-temperature electrical insulation and energy storage performance of polymer dielectrics. The self-assembly of high-insulation-performance boron nitride nanosheets (BNNS) on the film surface through electrostatic interactions effectively impedes charge injection from electrodes while promoting charge dissipation and heat transfer.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Zijian Li, Zhaohui Yang, Shao Wang, Hongxia Luo, Zhimin Xue, Zhenghui Liu, Tiancheng Mu
Summary: This study reports a strategy for upgrading polyester plastics into value-added chemicals using electrocatalytic methods. By inducing the targeted transfer of *OH species, polyethylene terephthalate was successfully upgraded into potassium diformate with high purity. This work not only develops an excellent electrocatalyst, but also provides guidance for the design of medium entropy metal oxides.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Navneet Singh Shekhawat, Surendra Kumar Patra, Ashok Kumar Patra, Bamaprasad Bag
Summary: This study primarily focuses on developing a sulphur dyeing process at room temperature using bacterial Lysate, which is environmentally friendly, energy and cost effective, and sustainable. The process shows promising improvements in dye uptake and fastness properties.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Dengjia Shen, Hongyang Ma, Madani Khan, Benjamin S. Hsiao
Summary: This study developed cationic PVC nanofibrous membranes with high filtration and adsorption capability for the removal of bacteria and hexavalent chromium ions from wastewater. The membranes demonstrated remarkable performance in terms of filtration efficiency and maximum adsorption capacity. Additionally, modified nanofibrous membranes were produced using recycled materials and showed excellent retention rates in dynamic adsorption processes.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Xiaoyan Wang, Zhikun Wang, Ben Jia, Chunling Li, Shuangqing Sun, Songqing Hu
Summary: Inspired by photosystem II, self-supported Fe-doped NiCoP nanowire arrays modified with carboxylate were constructed to boost industrial-level overall water splitting by employing the concerted proton-coupled electron transfer mechanism. The introduction of Fe and carboxyl ligand led to improved catalytic activity for HER and OER, and NCFCP@NF exhibited long-term durability for overall water splitting.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Pengyao Yu, Ge Yang, Yongming Chai, Lubomira Tosheva, Chunzheng Wang, Heqing Jiang, Chenguang Liu, Hailing Guo
Summary: Thin LTA zeolite membranes were prepared through secondary growth of nano LTA seeds in a highly reactive gel, resulting in membranes with superior permeability and selectivity in gas separation applications.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Baiqin Zhou, Huiping Li, Ziyu Wang, Hui Huang, Yujun Wang, Ruichun Yang, Ranran Huo, Xiaoyan Xu, Ting Zhou, Xiaochen Dong
Summary: The use of machine learning to predict the performance of specific adsorbents in phosphate adsorption shows great promise in saving time and revealing underlying mechanisms. However, the small size of the dataset and insufficient detailed information limits the model training process and the accuracy of results. To address this, the study employs a fuzzing strategy that replaces detailed numeric information with descriptive text messages on the physiochemical properties of adsorbents. This strategy allows the recovery of discarded samples with limited information, leading to accurate prediction of adsorption amount, capacity, and kinetics. The study also finds that phosphate uptake by adsorbents is generally through physisorption, with some involvement of chemisorption. The framework established in this study provides a practical approach for quickly predicting phosphate adsorption performance in urgent scenarios, using easily accessible information.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Engineering, Environmental
Paula Alejandra Lamprea Pineda, Joren Bruneel, Kristof Demeestere, Lisa Deraedt, Tex Goetschalckx, Herman Van Langenhove, Christophe Walgraeve
Summary: This study evaluates the use of four esterified fatty acids and three vegetable oils as absorption liquids for hydrophobic VOCs. The experimental results show that isopropyl myristate is the most efficient liquid for absorbing the target VOCs.
CHEMICAL ENGINEERING JOURNAL
(2024)