Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 61, Issue 4, Pages -Publisher
WILEY
DOI: 10.1002/mnfr.201600169
Keywords
Ellagitannins; NF-kappa B; Polyphenols; Punicalagins; Urolithin A
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Funding
- Procell sprl
- Walloon region
- United States Department of Agriculture ARS Specific Cooperative Agreement [58-6408-2-0009]
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Scope: We investigated whether punicalagin-rich pomegranate extract (PE) protects skeletal muscle of mice against inflammation induced by an acute injection of TNF-alpha. Results: Mice fed with PE or standard chow during 6 wk were injected with TNF-alpha (100 ng/g) or vehicle and sacrificed 6 h later. Prior supplementation with PE prevented the loss of tibialis anterior mass induced by TNF-alpha. In skeletal muscle, the activation of the NF-kappa B signaling and the induction of cytokines mRNA were reduced in mice having received PE. In those mice, the activity of the Akt/mTORC1 pathway and the protein synthesis were maintained after TNF-alpha injection whereas markers involved in the ubiquitin proteasome pathway were less activated. As urolithin A was the only punicalaginmetabolite detectable in plasma ofmice supplemented with PE, we performed in vitro experiments using a murine cell line (C2C12) to provide evidence that urolithin A is likely the active compound protecting skeletalmuscle against TNF-alpha-induced inflammation. Conclusion (focus on nutritional relevance): These results suggest that supplementation with a punicalagin-rich PE may protect skeletal muscle against an acute inflammation.
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