Site-Specifically Labeled Immunoconjugates for Molecular Imaging—Part 2: Peptide Tags and Unnatural Amino Acids

Hydrolytically Stable Site-Specific Conjugation at the N-Terminus of an Engineered Antibody

(2015) Pamela Thompson et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugates: The Nexus of Bioorthogonal Chemistry, Protein Engineering, and Drug Development

(2015) Paresh Agarwal et al.   BIOCONJUGATE CHEMISTRY

In vivo imaging with antibodies and engineered fragments

(2015) Amanda C. Freise et al.   MOLECULAR IMMUNOLOGY

Antibody Conjugates with Unnatural Amino Acids

(2015) Trevor J. Hallam et al.   MOLECULAR PHARMACEUTICS

Use of 18F-2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer

(2015) Mohammad Rashidian et al.   ACS Central Science

Enzyme-Mediated Site-Specific Bioconjugation of Metal Complexes to Proteins: Sortase-Mediated Coupling of Copper-64 to a Single-Chain Antibody

(2014) Brett M. Paterson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improving the Serum Stability of Site-Specific Antibody Conjugates with Sulfone Linkers

(2014) James T. Patterson et al.   BIOCONJUGATE CHEMISTRY

Chemoenzymatic Strategy for the Synthesis of Site-Specifically Labeled Immunoconjugates for Multimodal PET and Optical Imaging

(2014) Brian M. Zeglis et al.   BIOCONJUGATE CHEMISTRY

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugation through Glycoengineering

(2014) Qun Zhou et al.   BIOCONJUGATE CHEMISTRY

Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes

(2014) Penelope M. Drake et al.   BIOCONJUGATE CHEMISTRY

Derivatization of Antibody Fab Fragments: A Designer Enzyme for Native Protein Modification

(2014) Sandra Liebscher et al.   CHEMBIOCHEM

Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins

(2014) Kathrin Lang et al.   CHEMICAL REVIEWS

Site-Specific Trastuzumab Maytansinoid Antibody–Drug Conjugates with Improved Therapeutic Activity through Linker and Antibody Engineering

(2014) Thomas H. Pillow et al.   JOURNAL OF MEDICINAL CHEMISTRY

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

Optimising the radiolabelling properties of technetium tricarbonyl and His-tagged proteins

(2014) Adam Badar et al.   EJNMMI Research

Sortase-Tag Expressed Protein Ligation: Combining Protein Purification and Site-Specific Bioconjugation into a Single Step

(2013) Robert Warden-Rothman et al.   ANALYTICAL CHEMISTRY

Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells

(2013) Han Xiao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site Specific Discrete PEGylation of 124I-Labeled mCC49 Fab′ Fragments Improves Tumor MicroPET/CT Imaging in Mice

(2013) Haiming Ding et al.   BIOCONJUGATE CHEMISTRY

Mass Spectrometric Characterization of Transglutaminase Based Site-Specific Antibody–Drug Conjugates

(2013) Santiago E. Farias et al.   BIOCONJUGATE CHEMISTRY

Location Matters: Site of Conjugation Modulates Stability and Pharmacokinetics of Antibody Drug Conjugates

(2013) Pavel Strop et al.   CHEMISTRY & BIOLOGY

Methods for site-specific drug conjugation to antibodies

(2013) Christopher R Behrens et al.   mAbs

DOTA-Functionalized Polylysine: A High Number of DOTA Chelates Positively Influences the Biodistribution of Enzymatic Conjugated Anti-Tumor Antibody chCE7agl

(2013) Jürgen Grünberg et al.   PLoS One

Generation and Characterization of a Diabody Targeting the αvβ6 Integrin

(2013) Heide Kogelberg et al.   PLoS One

Sortase-mediated modification of αDEC205 affords optimization of antigen presentation and immunization against a set of viral epitopes

(2013) Lee Kim Swee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthesis of Heterobifunctional Protein Fusions Using Copper-Free Click Chemistry and the Aldehyde Tag

(2012) Jason E. Hudak et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

PET imaging with 89Zr: From radiochemistry to the clinic

(2012) Melissa A. Deri et al.   NUCLEAR MEDICINE AND BIOLOGY

Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR

(2012) S. A. Kazane et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Pictet-Spengler ligation for protein chemical modification

(2012) P. Agarwal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SNAP-Tag Technology Mediates Site Specific Conjugation of Antibody Fragments with a Photosensitizer and Improves Target Specific Phototoxicity in Tumor Cells

(2011) Ahmad Fawzi Hussain et al.   BIOCONJUGATE CHEMISTRY

Engineering of an anti-epidermal growth factor receptor antibody to single chain format and labeling by sortase A-mediated protein ligation

(2011) Mariusz P. Madej et al.   BIOTECHNOLOGY AND BIOENGINEERING

Site-specific modification of ED-B-targeting antibody using intein-fusion technology

(2011) Sina Möhlmann et al.   BMC BIOTECHNOLOGY

In vitro Sortagging of an Antibody Fab Fragment: Overcoming Unproductive Reactions of Sortase with Water and Lysine Side Chains

(2011) Sina Möhlmann et al.   CHEMBIOCHEM

A practical guide to the construction of radiometallated bioconjugates for positron emission tomography

(2011) Brian M. Zeglis et al.   DALTON TRANSACTIONS

Preclinical screening of anti-HER2 nanobodies for molecular imaging of breast cancer

(2011) Ilse Vaneycken et al.   FASEB JOURNAL

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

(2011) Cassandra Guarino et al.   GLYCOBIOLOGY

Site-Specific Coupling and Sterically Controlled Formation of Multimeric Antibody Fab Fragments with Unnatural Amino Acids

(2011) Benjamin M. Hutchins et al.   JOURNAL OF MOLECULAR BIOLOGY

Site-specific protein modifications through pyrroline-carboxy-lysine residues

(2011) W. Ou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-Specific and Stoichiometric Modification of Antibodies by Bacterial Transglutaminase

(2010) Simone Jeger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Chemoenzymatic methods for site-specific protein modification

(2010) David Rabuka   CURRENT OPINION IN CHEMICAL BIOLOGY

Conjugation and radiolabeling of monoclonal antibodies with zirconium-89 for PET imaging using the bifunctional chelate p-isothiocyanatobenzyl-desferrioxamine

(2010) Maria J W D Vosjan et al.   Nature Protocols

Site-specifically 89Zr-labeled monoclonal antibodies for ImmunoPET

(2010) Jeff N. Tinianow et al.   NUCLEAR MEDICINE AND BIOLOGY

Cell-free production of Gaussia princeps luciferase – antibody fragment bioconjugates for ex vivo detection of tumor cells

(2009) Kedar G. Patel et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Molecularly Defined Antibody Conjugation through a Selenocysteine Interface

(2009) Thomas Hofer et al.   BIOCHEMISTRY

Synthesis and Application of a N-1′ Fluorescent Biotinyl Derivative Inducing the Specific Carboxy-Terminal Dual Labeling of a Novel RhoB-Selective scFv

(2009) L. Chaisemartin et al.   BIOCONJUGATE CHEMISTRY

Multiple Site-Specificin VitroLabeling of Single-Chain Antibody

(2009) Boopathy Ramakrishnan et al.   BIOCONJUGATE CHEMISTRY

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY

Modification of Different IgG1 Antibodies via Glutamine and Lysine using Bacterial and Human Tissue Transglutaminase

(2007) Thomas L. Mindt et al.   BIOCONJUGATE CHEMISTRY