Journal
MOLECULAR BIOSYSTEMS
Volume 12, Issue 1, Pages 102-109Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5mb00627a
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Funding
- National Science and Technology Key Projects for Major Drug Innovation and Development [2013ZX09401002]
- National High Technology R&D Program (863 Program) of China [2012AA02A302]
- National Science Foundation of China (NSFC) [1470896]
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Chinese hamster ovary (CHO) cells are frequently used for the production of recombinant proteins for therapeutical applications. However, the recombinant protein expression level of CHO cells may reduce during long-term culture. The physiological changes related to the stability of expression were not well understood. In this study, we performed a series of genetic analysis on stable and unstable clonal derived populations. Transcriptome analysis revealed that a large number of differentially expressed genes (>100) were identified in the unstable population between early and late generations, while only a few differentially expressed genes were found in the stable population, suggesting that the gene expression change is related to the instability of recombinant protein production. On the other hand, no significant differences were found in promoter methylation or gene copy numbers in the unstable population. Taken together, our data help better understand the molecular mechanism underlying the stability of recombinant protein production in CHO cells.
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