Article
Biochemistry & Molecular Biology
Lusha Wei, Lei Liu, Ziwei Bian, Yifei Wu
Summary: In this study, the recombinant xylanase XynA was used to hydrolyze birchwood and beechwood xylans, and the polymerization distribution of xylo-oligosaccharides generated during enzymatic digestion was analyzed by MALDI-TOF-MS. Through enzyme hydrolysis, xylobiose and xylotriose were produced as potential food additives.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Microbiology
Maaike C. de Vries, B. J. A. Hoeve-Bakker, Maaike J. C. van den Beld, Amber C. A. Hendriks, Airien S. D. Harpal, Ramon C. E. A. Noomen, Frans A. G. Reubsaet
Summary: Francisella tularensis, a zoonotic bacterium, is not included in the standard library of the most commonly used mass spectrometry systems. By building an in-house Francisella library and defining specific biomarkers, F. tularensis can be accurately differentiated at subspecies level. These strategies can be used as a fast and specific method to identify F. tularensis in clinical laboratory setting.
Article
Food Science & Technology
Lijun Zhao, Lirong Jia, Bingcun Ma, Wei Zhong, Ying Huang, Feixia Duan
Summary: The study found that temperature significantly affects the texture stiffening of soybean curd, with high-temperature autoclaving leading to increased hardness. Analysis identified Bacillus, Clostridium, Lysinibacillus, and Brevibacillus as main contaminants. Bacterial contamination in two different processing lines was dominated by Staphylococcus, Macrococcus, Psychrobacter, Bacillus, and Acinetobacter.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Jianchun Wei, Huijuan Zhang, Huifang Zhang, Enmin Zhang, Binghua Zhang, Fei Zhao, Di Xiao
Summary: This study developed a rapid, high-throughput screening method using MALDI-TOF MS, coupled with ClinProTools, to differentiate between Bacillus anthracis and Bacillus cereus isolates. The classification model generated using a genetic algorithm accurately identified the bacteria with 100% sensitivity and specificity, making it a powerful tool for screening bacterial strains.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Victor Munoz-Hisado, Fatima Ruiz-Blas, Jesus Manuel Sobrado, Eva Garcia-Lopez, Emma Martinez-Alonso, Alberto Alcazar, Cristina Cid
Summary: The exploration of Mars is important for understanding its potential habitability. Recent studies have suggested the presence of liquid water in subglacial lakes below the South pole of Mars. In this study, two bacterial species were subjected to a simulated Martian environment to investigate their adaptation mechanisms. The results showed that the bacteria employed different strategies for survival.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Samart Dorn-In, Sirkka Mang, Karin Schwaiger
Summary: This study investigated cold-tolerant Clostridium spp. strains isolated from various samples, identifying them into different groups based on genetic and biochemical characteristics. Spiked beef samples showed changes in odor after storage, while maintaining similar color and meat quality compared to the control group.
Article
Food Science & Technology
Yaxin Gao, Danfeng Li, Zhiliang Tian, Lizhen Hou, Jie Gao, Bei Fan, Fengzhong Wang, Shuying Li
Summary: This study investigated the metabolite changes in soymilk fermented by Bacillus subtilis BSNK-5 using comparative metabolomics. The results showed that the metabolites were gradually enriched and stabilized with the extension of fermentation time. BSNK-5 effectively degraded the macromolecular substances of soymilk into small molecules, affecting the nutritional and functional properties of fermented soymilk.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Plant Sciences
Lucia Galovicova, Petra Borotova, Veronika Valkova, Nenad L. Vukovic, Milena Vukic, Margarita Terentjeva, Jana Stefanikova, Hana Duranova, Przemyslaw Lukasz Kowalczewski, Miroslava Kacaniova
Summary: The essential oil of Thymus serpyllum shows high free-radical scavenging and antimicrobial activity, especially against Pseudomonas aeruginosa, Salmonella enteritidis, and biofilm-forming bacteria. It also exhibits effective antifungal activity, particularly against Penicillium crustosum.
Article
Biochemistry & Molecular Biology
Lukas Hleba, Miroslava Hlebova, Anton Kovacik, Jana Petrova, Zuzana Maskova, Juraj Cubon, Peter Massanyi
Summary: This research aims to develop a rapid identification method for producing and non-producing strains of A.flavus based on the entire mass spectrum. The results show that it is possible to distinguish between AFB(1) producers and non-producers by comparing the entire mass spectra using MALDI-TOF MS. By establishing a local strain database, the identification of aflatoxigenic A.flavus strains can be even faster and cheaper.
Review
Microbiology
Eva Torres-Sangiao, Cristina Leal Rodriguez, Carlos Garcia-Riestra
Summary: Mass spectrometry (MS) has emerged as a powerful tool in clinical microbiology for rapid and reliable microbial identification. Its cost- and time-effective advantages make it a promising alternative to conventional methods, offering potential applications in early diagnosis and appropriate treatment of severe infections.
Article
Biochemical Research Methods
Sergio Fernando Huarachi, Gabriela Petroselli, Rosa Erra-Balsells, Marcela Carina Audisio
Summary: In this study, two Bacillus strains isolated from honey and a waste of an artisanal tannery were evaluated for their antibacterial activity against enteropathogenic Escherichia coli strains. The results showed that the crude cell-free supernatants of both Bacillus strains exhibited anti-E. coli activities, with the lipopeptide fraction of one strain showing significant inhibition. Analysis using mass spectrometry revealed that surfactin homologues were the most abundant lipopeptides produced by both strains, along with kurstakin, iturin, and fengycin homologues.
JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Microbiology
Kexin Liu, Yajie Wang, Minlei Zhao, Gaogao Xue, Ailan Wang, Weijie Wang, Lida Xu, Jianguo Chen
Summary: This study explored the potential of using MALDI-TOF MS protein profiles and machine learning methods to differentiate between different subspecies of Bifidobacterium. Five distinct biomarker peaks were identified using RFE, and machine learning models were developed. The RF model performed the best, surpassing other algorithms in terms of accuracy and sensitivity. The research findings suggest that this approach has the potential for rapid and precise discrimination of specific bacterial subspecies in commercial applications.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Eiseul Kim, Seung-Min Yang, Eun-Ji Cho, Hae-Yeong Kim
Summary: In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) coupled with specific peaks and databases was used to accurately classify five Lacticaseibacillus species. The discovery of species-specific protein peaks contributes to the high-resolution discrimination of these species.
Article
Biotechnology & Applied Microbiology
Manpreet Kaur, Rakesh Kumar, Poonam Katoch, Reena Gupta
Summary: In this study, lipase from a thermotolerant Bacillus subtilis TTP-06 was purified and characterized. The purified lipase showed high homogeneity and displayed specific activity. Its enzymatic properties and 3-D structure were also investigated.
Article
Plant Sciences
Jesieli Beraldo-Borrazzo, Julio Cesar Polonio, Rodrigo Pawloski Schoffen, Joao Arthur dos Santos de Oliveira, Andressa Domingos Polli, Benicio Alves de Abreu Filho, Elton Cruz, Jakeline Luiz Correa, Claudete Aparecida Mangolin, Maria de Fatima P. S. Machado
Summary: Bioprospecting of endophytic microorganisms from C. peruvianus plants revealed somaclones as a promising source for obtaining new biomolecules with biotechnological applications.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Agriculture, Dairy & Animal Science
Kuo-Lung Chen, Pei-Chun Tu, Chean-Ping Wu, Tsai-Tzu Chen, YaoChi Su, Sheng-Ya Chen, Chishih Chu
JOURNAL OF POULTRY SCIENCE
(2016)
Article
Agriculture, Dairy & Animal Science
Ruei Han Yeh, Chia Wen Hsieh, Kuo Lung Chen
Article
Agriculture, Dairy & Animal Science
Shih Yi Chen, Tsung Yu Li, Chih Heng Tsai, Dan Yuan Lo, Kuo Lung Chen
ANIMAL SCIENCE JOURNAL
(2014)
Article
Agriculture, Dairy & Animal Science
H. H. Wu, B. B. C. Weng, K. L. Chen, P. W. S. Chiou, B. Yu
Article
Agriculture, Dairy & Animal Science
K. -L. Chen, B. -C. Weng, M. -T. Chang, Y. -H. Liao, T. -T. Chen, C. Chu
Article
Agriculture, Dairy & Animal Science
K. L. Chen, T. Y. Lee, T. W. Chen, P. W. S. Chiou
Article
Agriculture, Dairy & Animal Science
K. -L. Chen, W. -L. Kho, S. -H. You, R. -H. Yeh, S. -W. Tang, C. -W. Hsieh
Article
Agriculture, Dairy & Animal Science
K. L. Chen, S. M. Tsay, P. W. S. Chiou, C. P. Sun, B. C. Weng
Article
Agriculture, Dairy & Animal Science
K. L. Chen, T. Y. Lee, C. C. Huang, Y. C. Chen, P. W. S. Chiou
Article
Agriculture, Dairy & Animal Science
Hsien-Juang Huang, Bor-Chun Weng, Yan-Der Hsuuw, Yueh-Sheng Lee, Kuo-Lung Chen
Summary: The study demonstrated that feeding pigs with 2.5-5% TSFP can improve average daily feed intake and feed conversion rate. The 5% TSFP group showed increased HDL-C, decreased LDL-C and blood urea nitrogen content, as well as enhanced lymphoblastogenesis, IFN-gamma and IgA production, and phagocytic cells oxygen burst capacity. This suggests that TSFP has positive effects on growth performance and immunity of finishing pigs.
Article
Biotechnology & Applied Microbiology
Ruei-Han Yeh, Chia-Wen Hsieh, Kuo-Lung Chen
Summary: This study aimed to investigate the dose tolerance of enzymatically degraded feather meal (EFM) in the diet and the effect of two-stage fermented feather meal on the growth performance and amino acid digestibility of broilers. The results showed that dietary inclusion of more than 15% EFM had a negative impact on growth performance, while the inclusion of Bacillius subtilis var. natto N21 + S. cerevisiae Y10 fermented EFM improved amino acid digestibility and broiler growth performance.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Hsien-Juang Huang, Yueh-Sheng Lee, Bor-Chun Weng, Cheng-Yung Lin, Yan-Der Hsuuw, Kuo-Lung Chen
Summary: This study investigated the effects of TSFP on the performance, clinical blood biochemistry, and immunity of sows and piglets. The results showed that the 2% and 3% TSFP groups had better weight gain and feed conversion ratio during gestation. In addition, the 3% TSFP group showed the best weaning litter weight and improved immune function.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Weng-Keong Lo, Ping-Lin Ong, Yueh-Sheng Lee, Yi-Tai Hsu, Kuo-Lung Chen
Summary: This study screened the proteolytic activity of Bacillus species in meat and bone meal and investigated the effects of fermented meat and bone meal-soybean meal products on the growth performance of broilers. The results showed that Bacillus subtilis M6 efficiently decomposed meat and bone meal-soybean meal and improved the protein properties and nutritional value of the product. Furthermore, supplementation with 5% fermented meat and bone meal-soybean meal products promoted weight gain in broilers.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Hsien-Juang Huang, Bor-Chun Weng, Yueh-Sheng Lee, Cheng-Yung Lin, Yan-Der Hsuuw, Kuo-Lung Chen
Summary: This study demonstrated that supplementing nursery pigs with 5% TSFP produced through a 32-hour first-stage fermentation using selected Bacillus strains resulted in improved growth performance and immunity.
FERMENTATION-BASEL
(2022)