Article
Cell Biology
Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Summary: The research identified TACC3 and clathrin as core members, and chTOG and GTSE1 as ancillary members of the complex. Additionally, PIK3C2A was shown not to be a part of the complex. Targeting the TACC3-clathrin interface or their microtubule-binding sites are the two most likely strategies to disrupt spindle stability mediated by this multiprotein complex.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Ying Zhang, Jing Zhu, Hao Xu, Qin Yi, Liang Yan, Liang Ye, Xinyuan Zhang, Min Xie, Bin Tan
Summary: Using real-time confocal microscopy and structured illumination microscopy, we observed the time-dependent internalization of fluorescently labeled S100B protein (S100B-Alexa488) into MSCs and its transport through endolysosomal pathways. Inhibitors and RNA interference approaches further demonstrated that clathrin and lipid rafts play important roles in the internalization of S100B-Alexa488, providing potential interventions for the safe application of MSCs in glioma therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Dustin T. King, Jesus E. Serrano-Negron, Yanping Zhu, Christopher L. Moore, Matthew D. Shoulders, Leonard J. Foster, David J. Vocadlo
Summary: Posttranslational modifications can alter protein stability and cellular physiology. This study presents an unbiased proteome-wide approach to explore the influence of protein modifications on thermodynamic stability. Using this approach, the authors identify a set of proteins that are destabilized by O-GlcNAc modification and form distinct macromolecular complexes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Matthew A. Birk, Noa Liscovitch-Brauer, Matthew J. Dominguez, Sean McNeme, Yang Yue, J. Damon Hoff, Itamar Twersky, Kristen J. Verhey, R. Bryan Sutton, Eli Eisenberg, Joshua J. C. Rosenthal
Summary: Temperature changes pose a challenge to the physiological function integration in poikilotherms. Among the behaviorally sophisticated coleoid cephalopods, the complex nervous systems face substantial problems in this aspect. RNA editing through adenosine deamination is a mechanism well-suited for environmental acclimation. This study reveals that Octopus bimaculoides' neural proteome undergoes significant reconfigurations via RNA editing in response to temperature changes, affecting over 13,000 codons and altering vital proteins.
Review
Cell Biology
Angela R. R. Bunning, Mohan L. L. Gupta Jr.
Summary: Accurate chromosome segregation is essential for cell and organismal viability. The mitotic spindle, composed mainly of dynamic microtubules, plays a crucial role in this process by forming a bipolar configuration and generating tension across sister kinetochores. Recent advances have shed light on the role of tension as a biophysical signal that ensures accurate chromosome segregation by destabilizing aberrant kinetochore-microtubule attachments and reinforcing correct connections.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Darshan Sapkota, Mandy S. J. Kater, Kristina Sakers, Kayla R. Nygaard, Yating Liu, Sarah K. Koester, Stuart B. Fass, Allison M. Lake, Rohan Khazanchi, Rana R. Khankan, Mitchell C. Krawczyk, August B. Smit, Susan E. Maloney, Mark H. G. Verheijen, Ye Zhang, Joseph D. Dougherty
Summary: Neuronal activity can regulate transcriptional and translational responses in astrocytes, providing an additional mechanism for astrocytes to dynamically modulate nervous system functioning.
Article
Cell Biology
Wendy A. Herbst, Weixian Deng, James A. Wohlschlegel, Jennifer M. Achiro, Kelsey C. Martin
Summary: By using nucleus-specific proteomic mapping in silenced and stimulated neurons, researchers revealed an understudied mechanism of nuclear proteome regulation: activity-dependent proteasome-mediated degradation. They found that the tumor suppressor protein PDCD4 undergoes rapid stimulus-induced degradation in the nucleus of neurons. This degradation of PDCD4 is crucial for normal activity-dependent transcription, impacting genes involved in synapse formation, remodeling, and transmission.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Microbiology
Shuying Chen, Fan Yang, Zixiang Zhu, Weijun Cao, Kaiqi Lian, Wei Zhang, Zhijian Zhu, Jijun He, Jianhong Guo, Xiangtao Liu, Bin Zhou, Haixue Zheng
Summary: The study reveals that foot-and-mouth disease virus can enter cells through endocytosis and endosomal pathways, even in the absence of integrin and heparan sulfate receptors. Inhibition of endocytosis can block viral internalization, while the acidic environment and the presence of dynamin are crucial for infection.
VETERINARY MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ugo Dionne, Anne-Claude Gingras
Summary: In recent years, proximity-dependent biotinylation approaches have been widely used to define the compositions of organelles and other structures. The contributions of post-translational modifications to protein compartmentalization, such as phosphorylation, have been discussed in this field.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Developmental Biology
Paula Vazquez-Pianzola, Dirk Beuchle, Gabriella Saro, Greco Hernandez, Giovanna Maldonado, Dominique Brunssen, Peter Meister, Beat Suter
Summary: BicD is essential for producing normal female meiosis II products, preventing re-entry of female meiotic products into the cell cycle, and pronuclear fusion. It plays a role in localizing SAC components for metaphase arrest and interacts with Clathrin heavy chain to correctly position proteins during female meiosis II.
Article
Multidisciplinary Sciences
Stylianos Papaioannou, Michael Dimitriou
Summary: During preparation for voluntary movements, goal-dependent modulation reduces human muscle afferent activity and also adjusts stretch reflex gains in shoulder and upper arm muscles. Central preparatory activity plays a role in tuning muscle stiffness through independent control of muscle spindle sensors based on task goals.
Article
Agronomy
Da Xiao, Jianxiu Yao, Xiwu Gao, Kun Yan Zhu
Summary: Clathrin-dependent endocytosis is essential for insect development and reproduction in Tribolium castaneum. The knockdown of four major genes involved in this endocytic pathway resulted in larval mortality and deformed adults with wing development defects. The findings provide potential molecular targets for insect pest management strategies.
PEST MANAGEMENT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Kun-Huan Zhang, Yuan-Jing Zou, Meng-Meng Shan, Zhen-Nan Pan, Jia-Qian Ju, Jing-Cai Liu, Yi-Ming Ji, Shao-Chen Sun
Summary: This study reveals the noncanonical functions of ADP-ribosylation factor 1 (Arf1) in mouse oocyte meiosis, including its roles in G2/M transition and meiotic spindle organization.
Article
Food Science & Technology
Haixi You, Juanrui Li, Yiju Li, Wei Wang, Zhipeng Yu, Jingbo Liu, Xuebo Liu, Long Ding
Summary: This study provides the first molecular-level evidence that clathrin-dependent endocytosis may be the main transport route of EWH in the intestinal epithelium. It demonstrates that EWH is absorbed intact through the intestinal epithelium into the bloodstream.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Engineering, Industrial
Makoto Kato, Yasuhiro Kakinuma
Summary: This research proposes a modeling method of CFRP spindle unit dynamics via generalized multipoint receptance coupling with contact characteristics at the spindle-holder interface. The temperature-dependent mechanical properties are analyzed by parameter identification from the model. The translational stiffness and damping of bearings in the CFRP spindle increase with temperature increase at high rotational speed.
CIRP ANNALS-MANUFACTURING TECHNOLOGY
(2023)
Article
Oncology
Rodney Luwor, Andrew P. Morokoff, Stephanie Amiridis, Giovanna D'Abaco, Lucia Paradiso, Stanley S. Stylli, Hong P. T. Nguyen, Mark Tarleton, Kelly A. Young, Terence J. O'Brien, Phillip J. Robinson, Megan Chircop, Adam McCluskey, Nigel C. Jones
CANCER INVESTIGATION
(2019)
Article
Chemistry, Multidisciplinary
Matthew G. Alteen, Christina Gros, Richard W. Meek, David A. Cardoso, Jil A. Busmann, Gontran Sangouard, Matthew C. Deen, Hong-Yee Tan, David L. Shen, Cecilia C. Russell, Gideon J. Davies, Phillip J. Robinson, Adam McCluskey, David J. Vocadlo
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Biochemistry & Molecular Biology
Hui Yi Chew, Priscila O. De Lima, Jazmina L. Gonzalez Cruz, Blerida Banushi, Godwins Echejoh, Lingbo Hu, Shannon R. Joseph, Benedict Lum, James Rae, Jake S. O'Donnell, Lilia Merida de Long, Satomi Okano, Brigid King, Rachael Barry, Davide Moi, Roberta Mazzieri, Ranjeny Thomas, Fernando Souza-Fonseca-Guimaraes, Matthew Foote, Adam McCluskey, Phillip J. Robinson, Ian H. Frazer, Nicholas A. Saunders, Robert G. Parton, Riccardo Dolcetti, Katharine Cuff, Jennifer H. Martin, Benedict Panizza, Euan Walpole, James W. Wells, Fiona Simpson
Article
Multidisciplinary Sciences
Rebecca C. Poulos, Peter G. Hains, Rohan Shah, Natasha Lucas, Dylan Xavier, Srikanth S. Manda, Asim Anees, Jennifer M. S. Koh, Sadia Mahboob, Max Wittman, Steven G. Williams, Erin K. Sykes, Michael Hecker, Michael Dausmann, Merridee A. Wouters, Keith Ashman, Jean Yang, Peter J. Wild, Anna deFazio, Rosemary L. Balleine, Brett Tully, Ruedi Aebersold, Terence P. Speed, Yansheng Liu, Roger R. Reddel, Phillip J. Robinson, Qing Zhong
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Cedric S. Tremblay, Sung Kai Chiu, Jesslyn Saw, Hannah McCalmont, Veronique Litalien, Jacqueline Boyle, Stefan E. Sonderegger, Ngoc Chau, Kathryn Evans, Loretta Cerruti, Jessica M. Salmon, Adam McCluskey, Richard B. Lock, Phillip J. Robinson, Stephen M. Jane, David J. Curtis
NATURE COMMUNICATIONS
(2020)
Correction
Multidisciplinary Sciences
Cedric S. Tremblay, Sung Kai Chiu, Jesslyn Saw, Hannah McCalmont, Veronique Litalien, Jacqueline Boyle, Stefan E. Sonderegger, Ngoc Chau, Kathryn Evans, Loretta Cerruti, Jessica M. Salmon, Adam McCluskey, Richard B. Lock, Phillip J. Robinson, Stephen M. Jane, David J. Curtis
NATURE COMMUNICATIONS
(2021)
Review
Hematology
Cedric S. Tremblay, Stephen B. Ting, Adam McCluskey, Phillip J. Robinson, David J. Curtis
Summary: Endocytosis plays a crucial role in controlling cellular processes and cellular responses to the microenvironment. Targeting therapeutic strategies based on endocytosis show promise for the treatment of hematological malignancies.
EXPERIMENTAL HEMATOLOGY
(2021)
Article
Chemistry, Medicinal
Luke R. Odell, Ngoc Chau, Cecilia C. Russell, Kelly A. Young, Jayne Gilbert, Phillip J. Robinson, Jennette A. Sakoff, Adam McCluskey
Summary: The study involved the synthesis of pyrimidine-based dynamin GTPase inhibitors, with di- and tri-substituted pyrimidines showing higher levels of dynamin inhibition and potentially stronger cytotoxicity. These inhibitors demonstrated a good correlation between dynamin inhibition and cytotoxicity in various cancer cell lines.
Article
Biochemical Research Methods
Stephen A. Wood, Peter G. Hains, Arnaud Muller, Melissa Hill, Susitha Premarathne, Mariyam Murtaza, Phillip J. Robinson, George D. Mellick, Alex M. Sykes
Summary: In this study, we quantified proteomic differences between healthy control and PD patient cells and identified the critical role of the unfolded protein response (UPR) in PD patients. PD patients with high amounts of PDIA6 and HYOU1 proteins were more sensitive to endoplasmic reticulum stress. These findings provide insight into underlying cellular dysfunctions in PD patients.
PROTEOMICS CLINICAL APPLICATIONS
(2022)
Article
Oncology
Emanuel Goncalves, Rebecca C. Poulos, Zhaoxiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker, Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yangxiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip J. Robinson, Qing Zhong, Mathew J. Garnett, Roger R. Reddel
Summary: The study analyzes the proteomes of multiple cancer cell lines through mass spectrometry and reveals thousands of cancer protein biomarkers that are not significant at the transcript level, demonstrating that the proteome has predictive power for drug response similar to the transcriptome.
Review
Biochemical Research Methods
Emma L. Boys, Jia Liu, Phillip J. Robinson, Roger R. Reddel
Summary: Tumor tissue processing methodologies combined with data-independent acquisition mass spectrometry (DIA-MS) can accurately analyze the proteome of multiple tumor samples, providing new insights into cancer classification, tumor biology, biomarkers, treatment response, and drug targets. However, these technologies have not yet been widely implemented in routine cancer clinical practice.
Article
Biochemical Research Methods
Rebecca C. Poulos, Zhaoxiang Cai, Phillip J. Robinson, Roger R. Reddel, Qing Zhong
Summary: Proteomic data are valuable for drug response prediction and biomarker discovery due to the direct interaction between most drugs and proteins in target cells. This review highlights the opportunities of combining large-scale proteomic data with drug-related research, focusing on oncology. Successful applications of drug response prediction using molecular data are discussed, along with the technical advances in data-independent acquisition mass spectrometry (DIA-MS) for biomarker discovery. The potential of machine learning in pharmacoproteomics and the challenges of clinical validation are also explored.
Article
Chemistry, Medicinal
Luke R. Odell, Mark J. Robertson, Kelly A. Young, Andrew B. McGeachie, Annie Quan, Phillip J. Robinson, Adam McCluskey
Summary: The Bis-T series of compounds are potent inhibitors of dynamin GTPase activity. However, their low cell permeability limits their activity. To overcome this, prodrug ester analogues were synthesized. Among them, propionic ester 4 showed the highest efficacy, as it could effectively cross the cell membrane and rapidly hydrolyze into the desired Bis-T-22.
Article
Chemistry, Medicinal
Peter J. Cossar, David Cardoso, Daniel Mathwin, Cecilia C. Russell, Beatrice Chiew, Michael P. Hamilton, Jennifer R. Baker, Kelly A. Young, Ngoc Chau, Phillip J. Robinson, Adam McCluskey
Summary: Wiskostatin is a specific and potent inhibitor of the N-WASP actin remodeling complex. It also inhibits dynamin GTPase activity and endocytosis.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Meeting Abstract
Oncology
Hui Yi Chew, Benedict Panizza, Caroline Cooper, Riccardo Dolcetti, Jim Coward, Adam McCluskey, Phillip J. Robinson, James W. Wells, Fiona Simpson
MOLECULAR CANCER RESEARCH
(2020)
