Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 54, Pages 10953-10956Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc03921e
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Funding
- ARC DECRA [DE 140101056]
- National Breast Cancer Foundation of Australia [CG-12-07]
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Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.
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