Journal
MICROCHIMICA ACTA
Volume 184, Issue 2, Pages 627-632Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-016-2050-x
Keywords
Kanamycin; Aptamer; Carbon nanotubes; Fluorescence Quenching; Milk analysis; Antibiotic
Categories
Funding
- fund from innovation fund of Jiangxi academy of agricultural sciences [2014CQN010]
- National quality and safety risk assessment of livestock and poultry products [GJFP2016007]
Ask authors/readers for more resources
The authors describe a fluorometric assay for the antibiotic kanamycin that is making use of a fluorescein-labeled aptamer against kanamycin and carbon nanotubes (CNTs). It is found that CNTs do not quench the fluorescence of the duplex formed between FAM-labeled aptamer and its complementary DNA (cDNA). However, if kanamycin is added, it will bind to the aptamer, and this leads to dehybridization of the duplex upon which the fluorescence of the label is quenched by the CNTs present in solution. The method has an analytical range that extends from 1.0 to 50 nmol L-1 of kanamycin, and the detection limit is 0.4 nmol L-1. It was successfully employed to the detection of kanamycin in (spiked) milk samples and gave good recoveries.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
