Microplate based assay for thrombin detection using an RNA aptamer as affinity ligand and cleavage of a chromogenic or a fluorogenic peptide substrate

Title
Microplate based assay for thrombin detection using an RNA aptamer as affinity ligand and cleavage of a chromogenic or a fluorogenic peptide substrate
Authors
Keywords
Photometry, Fluorescence, Toggle-25, Enzyme substrate, 2′-deoxyfluoro sugars, Microtiter plate, Kinetic assay, DNA aptamer, Nuclease resistance
Journal
MICROCHIMICA ACTA
Volume 183, Issue 6, Pages 1891-1898
Publisher
Springer Nature
Online
2016-03-21
DOI
10.1007/s00604-016-1833-4

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