Article
Biotechnology & Applied Microbiology
Kelei Zhao, Jing Li, Xiting Yang, Qianglin Zeng, Wei Liu, Yi Wu, Hui Zhou, Balakrishnan Prithiviraj, Xinrong Wang, Xikun Zhou, Yiwen Chu
Summary: Increasing evidence shows the polymicrobial nature of chronic infections, with bacterial pathogens frequently communicating and causing challenges for clinical therapy. Investigating bacterial interspecific interactions during antibiotic treatment is crucial for understanding polymicrobial infections and optimizing therapies. A study on the interaction between P. aeruginosa and S. aureus in the presence of commonly used antibiotics revealed that subinhibitory concentrations of cefotaxime and levofloxacin can influence P. aeruginosa survival in coculture by modulating its quorum-sensing system. This insight highlights the importance of the QS system in bacterial interactions and provides valuable information for clinical treatment strategies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Kieran Elmes, Astra Heywood, Zhiyi Huang, Alex Gavryushkin
Summary: Large-scale genotype-phenotype screens provide valuable data for identifying molecular alterations associated with a phenotype. This study focuses on the importance of epistatic effects in association studies, and aims to develop a method that can consider three-way interactions. The proposed method, Pint, outperforms known methods in simulated data and demonstrates accuracy in antibiotic resistance testing and siRNA perturbation screens.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Review
Microbiology
Amber Grace, Rajnish Sahu, Donald R. R. Owen, Vida A. A. Dennis
Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Biljana Mojsoska, Melanie Ghoul, Gabriel G. Perron, Havard Jenssen, Fatima AlZahra'a Alatraktchi
Summary: This study explores the impact of sub-inhibitory antibiotic concentrations on the production of virulence factors in environmental Pseudomonas aeruginosa strains, showing that sub-inhibitory antibiotic concentrations can alter bacterial toxin production, with strain-specific effects. Additionally, biologically relevant measured toxin concentrations were found to decrease the viability of cultured epithelial cells. This suggests that antibiotic-induced virulence should be considered in routine pathogen testing.
Article
Multidisciplinary Sciences
Nuno M. Oliveira, James H. R. Wheeler, Cyril Deroy, Sean C. Booth, Edmond J. Walsh, William M. Durham, Kevin R. Foster
Summary: In this study, we discovered that bacteria use pilus-driven motility to migrate towards antibiotics. Although these bacteria can reach high concentrations of antibiotics within a short period of time, they are unable to reproduce and eventually die. However, they release bacteriocins to attack other bacteria, indicating their response to antibiotics resembles that of competing colonies.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Yue Li, Lexin Xia, Jian Chen, Yulu Lian, Ajai A. Dandekar, Feng Xu, Meizhen Wang
Summary: The study reveals a significant increase in the minimum inhibitory concentration of evolved Pseudomonas aeruginosa PAO1 under sub-lethal conditions, associated with mutations in mpl and ampD, and overexpression of quorum sensing genes. The involvement of quorum sensing in antibiotic resistance evolution is highlighted, showing a multifactorial contribution to observed phenotypes.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Microbiology
Marta Hernandez-Garcia, Maria Garcia-Castillo, Sergio Garcia-Fernandez, Diego Lopez-Mendoza, Jazmin Diaz-Reganon, Joao Romano, Leonor Passaro, Laura Paixao, Rafael Canton
Summary: CrpP enzymes have been identified as a novel mechanism of ciprofloxacin resistance, with related genes found in a significant number of Pseudomonas aeruginosa isolates. Mutations in these genes, along with chromosomal mutations in gyrA and parC, contribute to ciprofloxacin resistance and the potential emergence of new variants. The spread of these genes in highly mobilizable genomic islands raises concerns about the dispersion of multidrug-resistant pathogens in hospital settings.
Article
Microbiology
Mona Bove, Mette Kolpen, Mads Lichtenberg, Thomas Bjarnsholt, Tom Coenye
Summary: In this study, the fitness, antimicrobial susceptibility, metabolic activity, gene expression, in vitro production of virulence factors and in vivo virulence of experimentally evolved Pseudomonas aeruginosa PAO1 were evaluated. Mutations in mexT and fusA1 were found in the evolved strains, leading to altered fitness, metabolic activity, mexE expression, and antimicrobial susceptibility. However, the in vitro production of virulence factors and virulence in vivo were unchanged compared to the wild-type strains. These evolved strains also showed reduced susceptibility to tobramycin in an in vivo mouse model. Overall, this study highlights the impact of experimental evolution on the fitness and antimicrobial susceptibility of Pseudomonas aeruginosa.
Article
Microbiology
Qi Liu, Liwen Yin, Chenjing Lv, Fang Bai, Zhihui Cheng, Weihui Wu, Yongxin Jin
Summary: Researchers have discovered that ciprofloxacin-resistant Pseudomonas aeruginosa can develop increased tolerance to the antibiotic through genetic mutations, including a mutation in the PA0625 gene which was not previously associated with ciprofloxacin resistance. This study enhances our understanding of ciprofloxacin resistance in P. aeruginosa.
Article
Engineering, Environmental
Katarzyna Slipko, Roberto B. M. Marano, Eddie Cytryn, Valentina Merkus, Markus Wogerbauer, Jorg Krampe, Edouard Jurkevitch, Norbert Kreuzinger
Summary: This study demonstrates that the core sludge microbiome in CAS systems is resilient to sub-inhibitory concentrations of ciprofloxacin, showing no measurable effects on antibiotic resistance determinants at a functional, structural, and antibiotic resistance levels.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Microbiology
Tatsuya Shirai, Mao Akagawa, Miho Makino, Manami Ishii, Ayaka Arai, Norika Nagasawa, Mitsuru Sada, Ryusuke Kimura, Kaori Okayama, Taisei Ishioka, Haruyuki Ishii, Shinichiro Hirai, Akihide Ryo, Haruyoshi Tomita, Hirokazu Kimura
Summary: Despite increasing evidence of clinical impact, the molecular evolution of the gene bla(PDC), which encodes Pseudomonas-derived cephalosporinase (PDC), remains unknown. Through evolutionary analysis, it was found that bla(PDC) diverged from a common ancestor approximately 4660 years ago, resulting in the formation of eight clonal variants. The functionality of PDC in antibiotic resistance remains similar regardless of genotype.
Article
Microbiology
Mengmeng Cheng, Ruiyi Chen, Lisheng Liao
Summary: This study investigates the impact of a gene variation, LasR(Q45stop), in Pseudomonas aeruginosa. The introduction of LasR(Q45stop) in the bacteria led to the development of elastase-phenotypic-variability variants during co-evolution with the wild-type. The variation in T2SS-peptidase xcpA and mexT genes played a crucial role in the divergence of various phenotypes. These findings highlight the ongoing microevolution of individual mutational trajectory diversity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Graziela De Araujo Lock, Victoria Etges Helfer, Bruna Bernar Dias, Bruna Gaelzer Silva Torres, Bibiana Verlindo De Araujo, Teresa Dalla Costa
Summary: This study investigated the concentrations of ciprofloxacin (CIP) in lung interstitial fluid and epithelial lining fluid (ELF) during acute and chronic infections of Pseudomonas aeruginosa. The results showed that acute infection had minimal impact on CIP distribution, while chronic infection led to decreased CIP concentrations in lung tissue. The evaluation demonstrated that CIP concentrations in plasma were sufficient for successful treatment of lung infections, but concentrations in lung tissue and ELF may be inadequate for effective treatment of chronic infection caused by biofilm-forming P. aeruginosa.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Microbiology
Congjuan Xu, Huimin Liu, Xiaolei Pan, Zhenzhen Ma, Dan Wang, Xinxin Zhang, Guangbo Zhu, Fang Bai, Zhihui Cheng, Weihui Wu, Yongxin Jin
Summary: The study conducted a detailed analysis of the molecular mechanisms behind the development of ciprofloxacin-resistant derivative of a clinical isolate, identifying specific genetic mutations responsible for increased antibiotic resistance in the isolate.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kamilla B. Alieva, Maria N. Golikova, Svetlana A. Dovzhenko, Mikhail A. Kobrin, Elena S. Strukova, Vladimir V. Ageevets, Alisa H. Avdeeva, Ofeliia Sulian, Sergey Sidorenko, Stephen Zinner
Summary: OXA-48 carbapenemases are frequently expressed by Klebsiella pneumoniae clinical isolates and decrease the effectiveness of meropenem therapy. The emergence of resistance in susceptible carbapenemase-producing isolates and their resistance patterns when exposed to meropenem are not completely understood. This study confirmed the applicability of the mutant selection window (MSW) hypothesis to meropenem and carbapenemase-producing K. pneumoniae.
Article
Soil Science
S. Khatri, S. Dubey, Y. S. Shivay, L. Jelsbak, S. Sharma
Summary: Organic farming, with its higher microbial activity and diversity, serves as a sustainable alternative in preventing soil-borne plant diseases. This study found that organic farming practices increased the abundance of biocontrol genera and diversity indices for bacterial communities in the soil. In planta experiments also showed that plants treated with microbiome from organic fields had lower disease severity compared to conventional fields. Key taxa such as Flavobacterium, Bacillus, Pseudomonas, and Planctomycetes were identified as having the potential to impart disease suppressiveness in organic fields. This research provides a basis for the development of synthetic microbial communities to induce disease suppression in otherwise conducive soils.
APPLIED SOIL ECOLOGY
(2023)
Article
Multidisciplinary Sciences
Yvonne Adams, Anne Skovsbo Clausen, Peter Ostrup Jensen, Malin Lager, Peter Wilhelmsson, Anna J. Henningson, Per-Eric Lindgren, Daniel Faurholt-Jepsen, Helene Mens, Peter Kraiczy, Kasper Norskov Kragh, Thomas Bjarnsholt, Andreas Kjaer, Anne-Mette Lebech, Anja R. Jensen
Summary: We screened a group of low-passage Borrelia burgdorferi s.l. isolates using a novel human-derived 3D blood-brain barrier (BBB)-organoid model. The results showed that human-derived BBB-organoids can be invaded by Borrelia spirochetes, leading to swelling and loss of structural integrity. The BBB-organoid model highlights the tropism between B. burgdorferi s.l. genospecies and their ability to cross the blood-brain barrier, contributing to central nervous system infection.
Article
Microbiology
Eva Armengol, Kasper Norskov Kragh, Tim Tolker-Nielsen, Josep M. Sierra, Doaa Higazy, Oana Ciofu, Miguel Vinas, Niels Hoiby
Summary: The combination of colistin and rifampicin has been found to effectively kill colistin-resistant Pseudomonas aeruginosa, suggesting it as a promising treatment for infections caused by this strain.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Magnus Bock, Anna Marie Theut, Johan G. C. van Hasselt, Hengzhuang Wang, Kurt Fuursted, Niels Hoiby, Christian Johann Lerche, Nikolaj Ihlemann, Sabine Gill, Ulrik Christiansen, Hans Linde Nielsen, Lars Lemming, Hanne Elming, Jonas A. Povlsen, Niels Eske Bruun, Dan Hofsten, Emil L. Fosbol, Lars Kober, Martin Schultz, Mia M. Pries-Heje, Jonas Henrik Kristensen, Jens Jorgen Christensen, Flemming S. Rosenvinge, Christian Torp Pedersen, Jannik Helweg-Larsen, Niels Tonder, Kasper Iversen, Henning Bundgaard, Claus Moser
Summary: Pharmacokinetic/pharmacodynamic analyses were conducted to assess the probabilities of target attainment for oral treatments of infective endocarditis. The majority of patients achieved the target level with orally administered antibiotics, supporting the efficacy of oral step-down antibiotic treatment in patients with infective endocarditis.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Yonggang Zhao, Dingqiang Chen, Boyang Ji, Xingju Zhang, Mikkel Anbo, Lars Jelsbak
Summary: A study conducted in two tertiary hospitals in Guangdong Province, China, analyzed the genomic epidemiology and antimicrobial resistance of Pseudomonas aeruginosa. The study found a high prevalence of multidrug-resistant and Carbapenem-Resistant Pseudomonas aeruginosa strains, as well as diverse sequence types and the presence of international high-risk clones. This study provides valuable insights for public health policies and measures for the prevention and control of Pseudomonas aeruginosa resistance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Yonggang Zhao, Dingqiang Chen, Kaichao Chen, Miaomiao Xie, Jiubiao Guo, Edward Wai Chi Chan, Lu Xie, Jingbo Wang, Enqi Chen, Sheng Chen, Weijun Chen, Lars Jelsbak
Summary: By sequencing and analyzing the genomes of 416 Pseudomonas aeruginosa strains from hospitals in China, the genetic, phenotypic, and transmission characteristics of carbapenem-resistant strains were elucidated, and the molecular signatures responsible for the increased prevalence of CRPA infections in China were identified. These findings may provide new insight into the development of effective strategies for worldwide control of CRPA and minimize the occurrence of untreatable infections in clinical settings.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Katrin Beilharz, Kasper Norskov Kragh, Blaine Fritz, Julius B. Kirkegaard, Tim Tolker-Nielsen, Thomas Bjarnsholt, Mads Lichtenberg
Summary: This protocol presents a method for assessing the metabolic activity of bacterial populations using isothermal calorimetry. It includes steps for preparing different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements. The protocol also uses principal component analysis and probabilistic logistic classification to differentiate between metabolic states of different populations and assess resemblance to wild-type bacteria. This fine-scale metabolic measurement method can contribute to the understanding of microbial physiology.
Article
Infectious Diseases
Magnus Bock, Johan G. C. Van Hasselt, Franziska Schwartz, Hengzhuang Wang, Niels Hoiby, Kurt Fuursted, Nikolaj Ihlemann, Sabine Gill, Ulrik Christiansen, Niels Eske Bruun, Hanne Elming, Jonas A. Povlsen, Lars Kober, Dan E. Hofsten, Emil L. Fosbol, Mia M. Pries-Heje, Jens Jorgen Christensen, Flemming S. Rosenvinge, Christian Torp-Pedersen, Jannik Helweg-Larsen, Niels Tonder, Kasper Iversen, Henning Bundgaard, Claus Moser
Summary: This study characterized the population pharmacokinetics of linezolid in patients with infective endocarditis and found that there is a substantial interaction between linezolid and rifampicin, which increases the clearance of linezolid. Model-based simulations showed that increasing the linezolid dose can compensate for the interaction without increasing the risk of adverse effects.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Evolutionary Biology
Melanie Ghoul, Sandra B. Andersen, Rasmus L. Marvig, Helle K. Johansen, Lars Jelsbak, Soren Molin, Gabriel Perron, Ashleigh S. Griffin
Summary: Pathogenic bacteria can evolve resistance and tolerance to antibiotic treatment. This study investigates the evolution of tolerance and resistance in Pseudomonas aeruginosa isolates from chronic cystic fibrosis lung infections. The results suggest that tolerance is positively selected in the lung and can act as a stepping stone to resistance. However, the association between resistance and tolerance is not inevitable and difficult to predict.
Article
Microbiology
Katrine Wegener Tams, Inge Larsen, Julie Elvekjaer Hansen, Henrik Spiegelhauer, Alexander Damm Strom-Hansen, Sophia Rasmussen, Anna Caecilia Ingham, Lajos Kalmar, Iain Robert Louis Kean, Oystein Angen, Mark A. Holmes, Karl Pedersen, Lars Jelsbak, Anders Folkesson, Anders Rhod Larsen, Mikael Lenz Strube
Summary: Antibiotics are widely used in pig farming, raising concerns about the selection of antibiotic-resistant bacteria and potential impact on human health. The study investigated the microbiome and resistome of pigs raised without antibiotics and those treated with antibiotics at a commercial farm. The results showed age-related changes in both the microbiome and resistome, as well as a temporary divergence between treated and untreated animals. The study suggests the need for further research to determine the optimal way to rear pigs without antibiotics.
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Lars Christophersen, Mette Kolpen, Peter Ostrup Jensen, Henrik Calum, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: Chronic wounds have compromised microcirculation and are often infested with microorganisms in biofilms, hindering antibiotic function. This study investigates whether hyperbaric oxygen therapy (HBOT) can counteract this process.
Article
Microbiology
Kendra P. Rumbaugh, Thomas Bjarnsholt
Summary: This article traces the historical development of biofilms and their significance in infections, discusses the importance and challenges of in vivo biofilm research, and summarizes the current understanding and knowledge gaps in biofilm infections.
Article
Infectious Diseases
Yonggang Zhao, Lu Xie, Chongzhi Wang, Qian Zhou, Lars Jelsbak
Summary: This study provides insights into the evolutionary pathways and routes of dissemination of Pseudomonas aeruginosa high-risk clones from Chinese and global perspectives. It reveals a geographical separation of isolates and identifies antibiotic resistance genes associated with this separation. Additionally, the evolutionary history of a China-specific high-risk clone is deduced.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: The study demonstrates that hyperbaric oxygen therapy (HBOT) can enhance the effectiveness of tobramycin in biofilms and counteract the micro-compartmentalization of biofilm pharmacokinetics through increased available tobramycin and augmented bacterial killing.
