Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 97, Pages 17225-17228Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc06817g
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Funding
- Biotechnology and Biosciences Research Council (BBSRC) [BB/L007444/1]
- Almac
- Novartis Pharmaceuticals
- Biotechnology and Biological Sciences Research Council [BB/L007444/1, BB/L010801/1] Funding Source: researchfish
- BBSRC [BB/L010801/1, BB/L007444/1] Funding Source: UKRI
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A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.
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