Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 23, Pages 4869-4871Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc10195b
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Funding
- [2 RO1 NS045062]
- [MSCRFF-0151-00]
- [MSCRFII-0042]
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Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.
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