Effects of cytoplasm from the related wild species Aegilops mutica on agronomic characters in Japanese bread wheat cultivars

Alloplasmic lines or cytoplasmic substitution lines of bread wheat (Triticum aestivum) that have cytoplasm from a related wild Aegilops species through recurrent backcrossing exhibit several useful characteristics for wheat breeding. Using an alloplasmic line initially developed by Professor Tsunewaki, we derived new alloplasmic lines for 14 Japanese bread wheat cultivars with Aegilops mutica cytoplasm to examine the effects of the cytoplasm on agronomic characters. All alloplasmic lines showed delayed heading time (4 to 17 days in the field) compared with the euplasmic lines, and the degree of heading delay depended on VERNALIZATION1 (VRN1) genotype. In spring wheat cultivars such as 'Minaminokaori' that carry the dominant VRN-A1 allele, the degree of heading delay due to the alien cytoplasm was large; by contrast, in winter wheat cultivars such as 'Haruibuki' that carry recessive vrn1 alleles in three homoeologous genes, the heading delay was small. Compared with euplasmic lines, the alloplasmic lines generally showed increased spikelet number per spike, but decreased floret number per spikelet, leading to decreased grain number per spike (GNS). However, GNS varied depending on genotype; in the alloplasmic lines of 'Nanbukomugi,' 'Nebarigoshi,' and 'Fukusayaka,' no decrease in GNS occurred. Furthermore, the 'Nebarigoshi' and 'Fukusayaka' alloplasmic lines could suppress the decrease in spike number per plant during winter due to delayed flowering. These alloplasmic lines will be useful for the development of varieties adapted to global warming.

Automated summary

Alloplasmic lines of bread wheat with cytoplasm from wild Aegilops species exhibit useful characteristics for wheat breeding, such as delayed heading time and increased spikelet number. The effects of the cytoplasm on agronomic characters differ depending on genotype, and different cultivars show variations in these traits.