Article
Biotechnology & Applied Microbiology
Roslina Jawan, Sahar Abbasiliasi, Joo Shun Tan, Murni Halim, Shuhaimi Mustafa, Bin Hao Lee, Jia Sim Kwa, Arbakariya B. Ariff
Summary: The study optimized parameters for the partitioning of bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 using a polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). The results showed that specific compositions of PEG and dextran in the ATPS significantly affected the partition coefficient, purification factor, and yield of BLIS, demonstrating the effectiveness of the system in extractive fermentation.
FERMENTATION-BASEL
(2021)
Article
Engineering, Chemical
Marcelo da Silva Pedro, Luiz Andre Fialho Oliveira, Carlos Eduardo de Araujo Padilha, Everaldo Silvino dos Santos, Jackson Araujo de Oliveira, Domingos Fabiano de Santana Souza
Summary: The study investigated the effects of flow patterns on the partitioning of biomolecules using microdevices in ATPE. Results showed that the droplet flow pattern exhibited better recovery and partition coefficient for BSA, while for ampicillin, the extraction performance was almost independent of the flow patterns.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Yuqiu Chen, Xiaodong Liang, Georgios M. Kontogeorgis
Summary: An optimal design method combining artificial neural network algorithm and group contribution method is developed in this study to predict the phase equilibrium composition and partition of biomolecules in aqueous two-phase systems (ATPS). By solving an optimization-based mixed-integer non-linear programming problem, the optimal ATPS is identified. Results show that our tailored ATPS exhibits significantly higher partitioning performance than reported ATPS in both cases.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Chemistry, Analytical
Qiaoshu Chen, Yanwen Zhang, Hui Chen, Jianbo Liu, Juewen Liu
Summary: Increasing the local concentration of DNA-based probes can improve the sensitivity of biosensors. A classic aqueous two-phase system (ATPS) using water soluble polymers is studied as a method to enrich DNA probes, leading to enhanced detection limits in molecular beacon-based DNA detection systems.
ANALYTICAL CHEMISTRY
(2021)
Article
Green & Sustainable Science & Technology
Dipak Kumar Kanaujiya, Kannan Pakshirajan
Summary: This study investigates the biodegradation of DMP and DEP using a TPPB system. The results demonstrate that Cellulosimicrobium funkei can efficiently degrade these endocrine disrupting chemicals, highlighting the potential of the TPPB system for detoxification in the environment.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Polymer Science
Maria Slyusarenko, Nadezhda Nikiforova, Elena Sidina, Inga Nazarova, Vladimir Egorov, Yuri Garmay, Anastasiia Merdalimova, Natalia Yevlampieva, Dmitry Gorin, Anastasia Malek
Summary: The aim of the study was to explore a two-phase polymer system formed in human plasma for isolating exosome-enriched fraction of plasma extracellular nanovesicles (ENVs). The study systematically analyzed the optimal combination of polymer mixture parameters and characterized the isolated vesicles. This new method for plasma ENV isolation shows applicability for diagnostic purposes.
Article
Biochemistry & Molecular Biology
Settimio Pacelli, Laura Di Muzio, Patrizia Paolicelli, Valeria Fortunati, Stefania Petralito, Jordan Trilli, Maria Antonietta Casadei
Summary: Cryogels are a special type of hydrogels with potential in drug delivery and tissue engineering. A novel cytocompatible cryogel has been developed as a spongy scaffold for the delivery of biomolecules, showing improved swelling, porosity, and mechanical properties compared to hydrogels. The cryogels are non-toxic and suitable for the delivery of water-soluble biomolecules, making them suitable for biomedical applications requiring macroporous scaffolds for localized delivery of bioactive molecules.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Thomas J. Tigner, Grant Scull, Ashley C. Brown, Daniel L. Alge
Summary: The phase diagram of a poly(ethylene glycol) (PEG) and dextran ATPS is related to the material properties of PEG microgel products. The polymer concentration of microgel pellets correlates with the average concentration of PEG in the ATPS. The mechanical properties of microgels rely on both the average concentration of PEG in the ATPS and the ATPS volume ratio.
Article
Chemistry, Multidisciplinary
Aceer Nadeem, Aidan Kindopp, Ian Wyllie, Lauren Hubert, James Joubert, Sophie Lucente, Ewelina Randall, Prakrit V. Jena, Daniel Roxbury
Summary: Supramolecular hybrids of DNA and single-walled carbon nanotubes (SWCNTs) have been widely used in biosensing applications due to their unique optical properties. Aqueous two-phase (ATP) purification methods have been introduced to increase the retention time, optical performance, and stability of DNA-SWCNTs within cells. The findings provide evidence of how cells process engineered nanomaterials depending on their state of purification, which can contribute to the development of more robust and sensitive biosensors in the future.
Article
Chemistry, Physical
Parisa Jafari, Abolghasem Jouyban
Summary: A systematic investigation of a biodegradable ATPS containing a natural deep eutectic solvent was conducted, showing the significant influence of the salting-out strength of the deep eutectic solvent on phase separation in the ATPS under different temperature and HBA:HBD molar ratios. High hydrophobic drugs could be effectively partitioned into the top phase, indicating a preferential interaction between the drugs and the polymer present in this phase.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Metallurgy & Metallurgical Engineering
Monserrat Martinez, Yahaira Barrueto, Yecid P. Jimenez
Summary: This work investigates the stripping of molybdenum from aqueous two-phase systems (ATPS) using polyethylene glycol (PEG) 4000, salt, and water under different pH and temperature conditions. Experimental tests were conducted using two molybdenum salts and various stripping agents. The results show that a solution of 20% (NH4)2SO4 as the phase former and 25% NH4OH as the pH modifier is the most effective stripping agent. The study also compared the stripping efficiencies of ATPS 1 and ATPS 2 and performed a pre-feasibility analysis that includes profit factor calculations.
Article
Thermodynamics
Keycianne da Cruz Silva, Leticia Daniela de Souza, Vinicius Azevedo Gomes, Leandro Rodrigues de Lemos
Summary: Phase diagrams of four aqueous two-phase systems were determined experimentally, and conclusions were drawn regarding the effect of temperature on system enthalpy change and the influence of macromolecules on phase separation.
FLUID PHASE EQUILIBRIA
(2024)
Article
Chemistry, Analytical
Yang Cao, Jingxuan Tian, Haisong Lin, Qingchuan Li, Yang Xiao, Huanqing Cui, Ho Cheung Shum
Summary: Most fluorescence-based bioanalytical applications require washing to remove excess labels, which is labor-intensive and hinders portable applications in resource-limited areas. In this study, an aqueous two-phase system (ATPS) is used for partitioning-induced isolation of labeled analytes without extra processing steps. The ATPS-based isolation is driven by affinity differences between analytes and excess labels. Different ATPS compositions were optimized for various scales of bioanalytical applications. The results showed successful isolation and analysis of analytes in centimeter-scale, millimeter-scale, and microscale ATPS.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ghazi Ben Messaoud, Sanja Aveic, Mattis Wachendoerfer, Horst Fischer, Walter Richtering
Summary: Porous hydrogels derived from aqueous two-phase systems (ATPS) are an interesting alternative to overcome the restricted porosity of most hydrogels in vitro. By tuning the phase behavior and concentration, ATPS hydrogels with three distinct microstructures, including bicontinuous hydrogels with interconnected pores, can be formed. These ATPS hydrogels show cytocompatibility and can sustain their unique porous structure when processed by inkjet and microextrusion techniques.
Article
Chemistry, Physical
Filipe S. Buarque, David E. M. Guimaraes, Cleide M. F. Soares, Ranyere L. Souza, Matheus M. Pereira, Alvaro S. Lima
Summary: This study investigated the phase separation in three different ethanol two-phase systems, analyzing the effects of polypropylene glycol molecular weight and ionic liquid alkyl chain length on phase formation, successfully achieving selective partitioning of curcumin and caffeic acid in saffron.
JOURNAL OF MOLECULAR LIQUIDS
(2021)