Article
Chemistry, Applied
Xiaohan Zhang, Zhao Huang, Dong An, Huajiang Zhang, Jing Wang, Ning Xia, Yanqiu Ma, Siyao Han, Afeng Wei
Summary: This study aimed to investigate the effects of alkali-heat and ultrasonic treatment on the structure, stability, and polyphenol encapsulation ability of OVA. The results showed that proteins treated with alkali-heat and ultrasound had higher stability and surface hydrophobicity, leading to changes in their functional properties. The generation of S-OVA through alkali-heat treatment exhibited higher thermal stability and softer gel formation. Both treatments enhanced the binding ability of OVA with polyphenols, resulting in increased antioxidant capacity. This study provides insights into the binding mode of OVA with polyphenols and the structural changes in proteins under different treatments, highlighting the advantages of alkali-heat and ultrasonic treatment of OVA in the food industry.
FOOD HYDROCOLLOIDS
(2023)
Article
Biochemistry & Molecular Biology
Xuan Zhou, Aamir Iqbal, Jiaxing Li, Chang Liu, Ayesha Murtaza, Xiaoyun Xu, Siyi Pan, Wanfeng Hu
Summary: This study investigated the browning degree and reducing power of catechin, epicatechin, caffeic acid, and chlorogenic acid in autoxidation and enzymatic oxidation. Results showed that polyphenols have different chemical characteristics in autoxidation and enzymatic oxidation, with enzymatic oxidation products having higher reducing power than autoxidation products, and polyphenols being more prone to autoxidation browning in an alkaline environment.
Article
Food Science & Technology
Le Ao, Panhang Liu, Annan Wu, Jing Zhao, Xiaosong Hu
Summary: The study established a molecular docking and virtual screening method to investigate the interaction between soybean protein isolate (SPI) and polyphenols, with results showing that the binding pattern is highly dependent on the detailed structure of polyphenols. Flavonoids were found to exhibit higher binding capacity than phenolic acids, with hydrogen bonding and hydrophobic interactions being the dominant forces in SPI-polyphenol binding. Cyanidin chloride demonstrated the highest apparent binding constant among the top-ranking polyphenols.
Article
Biochemistry & Molecular Biology
Sujata Choudhari, Sachin Kumar Patil, Sanket Rathod
Summary: This study aimed to identify polyphenols that can inhibit human pancreatic lipase and potentially be used for obesity treatment. Through various methods, four potential candidates were identified. The findings provide insights into the inhibitory potential of selected polyphenols on human pancreatic lipase and support further experimental investigations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Applied
Hengle Zhou, Shenke Bie, Jian Li, Lei Yuan, Linyan Zhou
Summary: The inhibition mechanisms of soluble and membrane-bound polyphenol oxidases (PPOs) by various inhibitors were studied using multispectroscopic analysis and computational simulations. Reduced glutathione exhibited the strongest inhibitory effect. The inhibitors disrupted the secondary and tertiary structures of the enzymes, with soluble PPO being more susceptible. Docking and simulation results provided insights into the different inhibition mechanisms of PPOs.
Article
Chemistry, Applied
Qian-Yun Han, Fang Liu, Xin Wen, Yuan-Ying Ni
Summary: The inhibitory effect of four selected compounds on membrane-bound polyphenol oxidases was demonstrated to be reversible. Fluorescence spectroscopy revealed a single class of inhibition site on the enzyme. Important binding sites in the active center were identified using molecular docking techniques.
Article
Acoustics
Yimei Zheng, Boyu Chen, Xuanxiang Huang, Hui Teng, Chao Ai, Lei Chen
Summary: The influence of ultrasound-assisted free radical modification on the structure and functional properties of OVA-EGCG conjugates was investigated. Ultrasound-assisted processing significantly increased the conjugating efficiency of OVA and EGCG and shortened the conjugating time. Covalent conjugates could be formed between OVA and EGCG, and modification in the conformation of OVA was induced by EGCG. Functional properties of OVA, including emulsification and antioxidant properties, were significantly improved after conjugation with EGCG, especially in ultrasound-assisted conditions.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Engineering, Environmental
Wenjing Lu, Chunhui Zhang, Zhiwei Lin, Xuezhi Li, Guifeng Zhao, Zhaowei Qin, Tengzhuo Zhang, Yiyun Li, Wenyang Li, Yizhen Zhang
Summary: This study prepared amyloid fibril hybrids with significant fluoride removal capacity and demonstrated their stability and high efficiency in real wastewater experiments. The secondary conformation of proteins changed during material synthesis and fluoride adsorption, and the synergistic effects between amyloid fibrils and ZrO2 played a key role in fluoride removal.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Matthias Menzel, Andreas Kiesow, Juliana Martins de Souza E. Silva
Summary: Dentin hypersensitivity is a common oral health concern, and occlusion of exposed dentinal tubules is a treatment option. Current microscopic techniques have limitations in evaluating the occluding effects of desensitizing agents. This study used high-resolution X-ray computed tomography (nano-CT) to investigate human dentin treated with silver diammine fluoride (SDF). Nano-CT provided comparable resolution to scanning electron microscopy (SEM) and produced three-dimensional images of the occluded tubules.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Applied
Hongwei Jiang, Lei Zhou, Yuefang Sun, Kaibo Yu, Wenzhi Yu, Yuqing Tian, Junping Liu, Liqiang Zou, Wei Liu
Summary: This study found that acidic pH could enhance the inhibitory effects of phenolic acids and flavonoids on polyphenol oxidase. The results showed that 4-hydroxycinnamic acid had a greater enhancement effect, which was supported by fluorescence emission spectra and circular dichroism experiments.
Article
Multidisciplinary Sciences
Ryouichi Satou, Atsushi Yamagishi, Atsushi Takayanagi, Seitaro Suzuki, Dowen Birkhed, Naoki Sugihara
Summary: In this study, the effectiveness of the toothpaste technique in preventing caries in home care was investigated. By diluting toothpaste samples and measuring viscosity, it was found that at low concentrations, toothpaste can be easily washed out of high-risk interdental areas, resulting in reduced caries prevention. The study proposed a method to maintain toothpaste in the interdental area and showed that using finger brushes can increase the efficiency of toothpaste delivery.
Article
Chemistry, Applied
Qi Yuan, Lin He, Xuping Wang, Huaigu Yang, Fan Zhu, Xiaoxia Peng, Jingrong Cheng, Yaosheng Lin, Daobang Tang, Xueming Liu
Summary: The interaction mechanism of components in complex food systems, such as the interaction between mulberry polyphenols (MP) and beta-lactoglobulin (beta-LG) studied in this paper, plays an important role in food science. The study found that these polyphenols interacted with beta-LG through static quenching, affecting the particle size, secondary structure, and stability of the protein.
FOOD HYDROCOLLOIDS
(2024)
Article
Biochemistry & Molecular Biology
Shancan Wang, Mingyu Li, Beibei He, Yueyuan Yong, Jie Zhu
Summary: Currently, food waste and the potential hazards of petroleum-based food packaging films have become serious issues, leading to increased focus on the development of new food packaging materials. In this study, a novel packaging film based on sodium alginate and konjac glucomannan blended with tea polyphenols was prepared. The film exhibited excellent microstructure, mechanical properties, barrier property, oxidation property, antibacterial activity, and stability. The results also showed that tea polyphenols could affect bacterial growth by interacting with peptidoglycan in the cell wall. Additionally, the film demonstrated excellent preservation effects in beef and apples, indicating its potential as a bioactive packaging material for food preservation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Jingfang Li, Ze-yuan Deng, Yushan He, Yawei Fan, Huanhuan Dong, Ronghua Chen, Ronghua Liu, Rong Tsao, Xiaoru Liu
Summary: In this study, lotus seed PPO was purified and studied through molecular docking, revealing that (-)-epicatechin is the optimal substrate for lotus seed PPO due to its stronger interaction with the enzyme.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shakti Chandra Vadhana Marimuthu, Jayaprabhakaran Murugesan, Ewa Babkiewicz, Piotr Maszczyk, Murugesan Sankaranarayanan, Esakkimuthu Thangamariappan, Joseph Christina Rosy, Sureshbabu Ram Kumar Pandian, Selvaraj Kunjiappan, Vanavil Balakrishnan, Krishnan Sundar
Summary: In this study, pharmacoinformatics techniques were used to screen and identify effective phytochemicals that can target specific proteins involved in the quorum sensing pathway of oral pathogen Streptococcus mutans. The computational approach involved homology modeling, molecular docking, and molecular dynamics simulations. The results demonstrated the strong binding affinity and stability of the identified compounds with the target proteins.