Article
Chemistry, Multidisciplinary
Dino Gluhacevic von Kruchten, Magdalena Roth, Oliver Seitz
Summary: This study introduces a new reaction design involving the template-controlled cleavage of PNA-spermine conjugates, allowing for catalytic activity and high efficiency. It has potential applications in DNA-encoded drug discovery and nucleic acid diagnostics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Yuetong Yang, Derong Kong, Yungen Wu, Yiheng Chen, Changhao Dai, Chang Chen, Junhong Zhao, Shi Luo, Wentao Liu, Yunqi Liu, Dacheng Wei
Summary: A graphene field-effect transistor (GFET) biosensor based on a tetrahedral DNA nanostructure (TDN)-assisted catalytic hairpin assembly (CHA) reaction (TCHA) has been developed, allowing sensitive and specific detection of miRNA-21. The TDN structures in the biosensing interface facilitate the CHA reaction and prevent unwanted entanglements, aggregation, and adsorption of probes on the graphene channel. The biosensor exhibits ultra-sensitive detection of miRNA-21, with a detection limit of 5.67 x 10(-19) M, three orders of magnitude lower than that achieved by graphene transistors with single-stranded DNA probe functionalization of the channel. Furthermore, the biosensor demonstrates excellent performance in terms of selectivity, stability, and reproducibility, showing promising potential in bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Li-Ping Jia, Rui-Juan Zhao, Zhe Feng, Ming-Yue Wang, Rong-Na Ma, Wen-Li Jia, Lei Shang, Wei Zhang, Qing-Wang Xue, Huai-Sheng Wang
Summary: Tumor biomarker detection is crucial for cancer early diagnosis and treatment. In this study, hollow polymeric nanospheres with high loading efficiency of ferrocene molecules were prepared, and an ultrasensitive electrochemical biosensor for circulating tumor DNA detection was developed by combining dual-enzyme assisted target amplification strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Yilin Liu, Li Zhan, Zhenpeng Qin, James Sackrison, John C. Bischof
Summary: LFAs, paper-based point-of-care diagnostic tools, have traditionally lower sensitivities and specificities than standard laboratory tests. Recent efforts have focused on optimizing assay kinetics, signal amplification, and sample preamplification to increase sensitivities, as well as improving specificity through high-affinity molecules and assay optimization. With continuing improvements, LFAs may soon offer performance competitive with laboratory techniques while retaining a rapid format.
Article
Chemistry, Analytical
Bo Shen, Li Li, Changjin Liu, Xinmin Li, Xinyu Li, Xiaoxue Cheng, Haiping Wu, Tiantian Yang, Wei Cheng, Shijia Ding
Summary: An ultrasensitive electrochemiluminescent (ECL) biosensor was constructed for accurate detection of exosomal microRNAs (exomiRNAs), using a three-dimensional (3D) walking nanomotor-mediated CRISPR/Cas12a and tetrahedral DNA nanostructures (TDNs)-modified nanoemitters (TCPP-Fe@HMUiO@Au-ABEI). The biosensor achieved a limit of detection down to 273.20 aM and successfully discriminated breast cancer patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Chunhuan Xu, Jingjin Zhao, Shengyu Chen, Ivan Yu Sakharov, Shengqiang Hu, Shulin Zhao
Summary: A novel DNA machine-based approach for absolute quantification of mRNA in individual cells is presented. This method provides important insights into cellular function and has great research and diagnostic value.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yunlong Liu, Xiaoming Wang, Yujiao Li, Haiping Wu
Summary: This paper proposes a temperature-programmed enzymatic reaction method for cfDNA methylation analysis in a single tube. The method uses endonuclease to mildly recognize DNA methylation to avoid cfDNA degradation, and two stages of amplification reactions significantly improve detection sensitivity for GC-rich sequences. The method accurately quantifies the methylation level of the target sequence and successfully detects the methylated vimentin gene in 20 clinical plasma samples. The results suggest that the proposed method has great application potential as a complement to bisulfite conversion-based methods for DNA methylation analysis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hong Jiang, Li-Bin Wang, Ya-Ting Zhang, Min Dong, Jian Li, Ji-Dong Wang
Summary: In this study, a simple fluorescent signal amplification biosensor based on an entropy-driven three-dimensional DNA walker was developed for highly sensitive detection of cancer cells. The biosensor showed excellent selectivity and sensitivity, and has great potential for early diagnosis and clinical treatment of cancer.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Juan Song, Shan Li, Jie Zhou, Qiao Yu, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We have developed a triple signal amplification strategy for in situ visualization of telomerase RNA (TR) in living cells. The strategy includes target-initiated strand displacement circuit, multidirectional rolling circle amplification (RCA), and Mg2+ DNAzyme-mediated amplification. The proposed method exhibited ultrasensitive detection of TR mimic with a detection limit at the picomolar level. Moreover, after transfection with the proposed sensing platform, tumor cells can be easily distinguished from normal cells based on TR abundance-related fluorescence signal, providing new insights into initial cancer screening.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Qingyu Wang, Haobo Sun, Dongxiao Wen, Lei Wang, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: An electrochemical sensing platform based on ATRP signal amplification strategy has been developed for ultrasensitive determination of breast and prostate cancer marker miRNA-141, with a linear range from 10 pM to 10 aM and a detection limit of 3.23 aM, providing a new and promising path for ultrasensitive analysis of tumor-related miRNAs.
Article
Biophysics
Yuecan Zhao, Junzhu Xiang, Hao Cheng, Xiaojuan Liu, Feng Li
Summary: The development of a novel flexible photoelectrochemical biosensor for miRNA determination showed promising potential for ultrasensitive disease diagnosis. The biosensor exhibited excellent analytical performance, selectivity, stability, flexibility, and practical applicability, making it a valuable tool for bioassays and early disease detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Kexin Ding, Yantian Shi, Yaojia Liu, Xinyue Song, Haiyan Wang, Jing Liu, Hong Zhou
Summary: We developed an enzyme-assisted multiple signal amplification reaction for target nucleic acid detection. This strategy involves utilizing the target nucleic acid as triggers, along with specific primers, DNA polymerase, and DNA nicking endonuclease, to promote repetitive cycle DNA replication and exponentially increase the nucleic acid sequence. The amplification products form a Mg2+-DNAzyme unit with the specific primer, enabling real-time fluorescence detection of HIV-1 DNA through cyclic cleavage of hairpin probes. Under optimal conditions, this method has a detection limit of 10-17 M. It offers a simple design, isothermal conditions, and easy fluorescence measurement, making it highly sensitive and specific for quantitative detection of DNA or RNA in theoretical and clinical studies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Yihan Wang, Huan Feng, Ke Huang, Jinfeng Quan, Xiaohui Liu, Hui Jiang, Xuemei Wang
Summary: In this study, a signal amplification sensing system with target-triggered DNA cascade reaction and dual-signal readout technology was designed for ultrasensitive analysis of miRNA. The system utilized highly conductive metal organic frameworks, tetrahedral DNA nanostructure, and non-enzymatic amplification method to improve sensitivity, and showed promising application in complex biological systems.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Siqi Zhang, Wei Shi, Kai-Bin Li, De-Man Han, Jing-Juan Xu
Summary: In this study, an ultrasensitive and label-free method for detecting DNA adenine methyltransferase (Dam) and CpG methyltransferase (M.SssI) was developed using the nanopore technique coupled with DNA cascade signal amplification reactions. The method showed high sensitivity and excellent specificity for the detection of methyltransferases.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Hao Jiang, Xuefei Lv, Yuan Li, Yulin Deng, Shiyong Yu
Summary: Rapid and accurate detection of pathogens is crucial for the prevention, control, and diagnosis of infectious diseases. In this study, an ultrasensitive nucleic acid isothermal cascade amplification technique combining rolling circle amplification (RCA) and hybridization chain reaction (HCR) was developed for the detection of ORF1ab in SARS-CoV-2. The proposed method showed a detection limit as low as 7.65 fM and demonstrated satisfactory recoveries in serum samples. This facile and ultrasensitive assay provides a promising tool for ORF1ab analysis and can be applied to the detection of various pathogens and genetic biomarkers.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Physical
Xiaoli Zhu, Xiaoxia Mao, Zihan Wang, Chang Feng, Guifang Chen, Genxi Li
Article
Chemistry, Analytical
Xiaonan Wang, Ying Liu, Juan Zhang, Genxi Li
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Medicine, Research & Experimental
Chang Feng, Bing Bo, Xiaoxia Mao, Hai Shi, Xiaoli Zhu, Genxi Li
Article
Chemistry, Physical
Jin Jia, Weijia Zhou, Zhaoqian Wei, Tanli Xiong, Guixiang Li, Lili Zhao, Xiaofei Zhang, Hong Liu, Jian Zhou, Shaowei Chen
Article
Medicine, Research & Experimental
Xiaoxia Chen, Jing Zhao, Tianshu Chen, Tao Gao, Xiaoli Zhu, Genxi Li
Article
Multidisciplinary Sciences
Chao Li, Liu Shi, Yaqin Tao, Xiaoxia Mao, Yang Xiang, Genxi Li
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Analytical
Yuxin Lin, Beibei Nie, Xinyu Qu, Minghui Wang, Jie Yang, Genxi Li
Summary: Covalent organic frameworks (COFs) have attracted much attention for their unique physical-chemical characteristics and potential applications in biosensing. In this study, spherical COFs with regular shape and good dispersion were synthesized, and a novel nanoprobe was developed by modifying Histostar on the COFs surface. The nanoprobe was then applied to fabricate an electrochemical biosensor for exosomes detection, achieving a low detection limit and wide linear detection range. This work offers a promising platform for ultrasensitive detection of exosomes.
Article
Chemistry, Analytical
Cao Ya, Kang Ming-Yang, Chen Hong, Tang Ying-Ying, Feng Chang, Zhao Jing, Li Gen-Xi
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Juan Zhang, Xiaonan Wang, Tingjun Chen, Chang Feng, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Chao Li, Yaqin Tao, Yi Yang, Chang Feng, Yang Xiang, Genxi Li
Article
Chemistry, Analytical
Tao Gao, Bei Wang, Liu Shi, Xiaoli Zhu, Yang Xiang, Jun-ichi Anza, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Electrochemistry
Tao Gao, Shiyu Gu, Chaoli Mu, Meiling Zhang, Jie Yang, Ping Liu, Genxi Li
ELECTROCHIMICA ACTA
(2017)
Article
Chemistry, Multidisciplinary
Xiaolu Hu, Chao Li, Chang Feng, Xiaoxia Mao, Yang Xiang, Genxi Li
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Analytical
Xiaoli Zhu, Yaoyao Chen, Chang Feng, Wei Wang, Bing Bo, Ruixin Ren, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Jinlong Li, Tao Gao, Shiyu Gu, Jun Zhi, Jie Yang, Genxi Li
BIOSENSORS & BIOELECTRONICS
(2017)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
