Article
Cell Biology
Tingting Gan, Yuhong Wang, Yang Liu, David G. Schatz, Jiazhi Hu
Summary: RAG1 and RAG2 form a tetramer nuclease for V(D)J recombination in lymphocytes, with RAG1 requiring interaction with RAG2 to be activated. RAG2 disrupts RAG1 aggregates, activating recombination.
Article
Immunology
Julia J. Reichwald, Frederic Risch, Anna-Lena Neumann, Stefan J. Frohberger, Johanna F. Scheunemann, Benjamin Lenz, Alexandra Ehrens, Wiebke Strutz, Beatrix Schumak, Achim Hoerauf, Marc P. Huebner
Summary: This study investigated the role of ILC2s during filarial infection using a rodent model of Litomosoides sigmodontis. The results showed that ILC2s were increased in C57BL/6 mice, accompanied by a stronger type 2 immune response. Furthermore, ILC2s were found to be essential in controlling microfilaremia in Rag2(-/-) C57BL/6 mice.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Plant Sciences
Daniel Dastan Rezabala Pacheco, Brenda Conceicao Guimaraes Santana, Carlos Priminho Pirovani, Alex-Alan Furtado de Almeida
Summary: This article presents a detailed study on the ZIP gene family in plants, which is involved in transporting cationic elements. The TcZIP genes in cacao underwent duplication and purifying selection, and the proteins have specific conformation and metal binding site. The analysis also revealed their interaction and enrichment in cation transport.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Immunology
Heng Zhao, Weijian Ye, Jianxiong Guo, Jiaoxiang Wang, Deling Jiao, Kaixiang Xu, Chang Yang, Shuhan Chen, Muhammad Ameen Jamal, Zhongbin Bai, Taiyun Wei, Jie Cai, Tien Dat Nguyen, Yubo Qing, Wenmin Cheng, Baoyu Jia, Honghui Li, Hong-Ye Zhao, Qingfeng Chen, Hong-Jiang Wei
Summary: We generated RGFKO pigs via CRISPR/Cas9 system and somatic cell nuclear transfer, which are both immune deficient and constantly suffer liver injury. These pigs have the potential to be engrafted with human hepatocytes without immune rejection, allowing for large scale expansion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ayeong So, Elodie Dardillac, Ali Muhammad, Catherine Chailleux, Laura Sesma-Sanz, Sandrine Ragu, Eric Le Cam, Yvan Canitrot, Jean Yves Masson, Pauline Dupaigne, Bernard S. Lopez, Josee Guirouilh-Barbat
Summary: Selection of DSB repair pathway is crucial for genetic stability. RAD51 plays a role in preventing nonconservative repair by controlling the second step of the repair process in human cells. RAD51's DNA binding ability is required for inhibiting SSA/A-EJ, not GC.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Genetics & Heredity
John Murray Topp Hunt, Christopher Allan Samson, Alex du Rand, Hilary M. Sheppard
Summary: The CRISPR-Cas gene-editing system is a valuable tool for research purposes and has made significant advancements in human clinical trials. However, our understanding of the editing outcomes has not kept pace with the developments in generating edits. Comprehensive validation of on- and off-target effects is necessary to ensure reproducibility and accurately assess safety in future editing applications.
Article
Biochemistry & Molecular Biology
Yiqing Ma, Fangrui Lou, Xiaofei Yin, Bailin Cong, Shenghao Liu, Linlin Zhao, Li Zheng
Summary: This study utilized whole-genome sequencing data to investigate the genomic characteristics and phylogenetic relationship of Pacific cod (Gadus macrocephalus). The study revealed that the estimated genome size of Pacific cod was approximately 658.22 Mb, and it contained a high proportion of repetitive sequences. Analysis of the mitochondrial genome provided insights into the evolutionary relationships between Pacific cod and its relatives. Additionally, demographic history analysis indicated changes in the population of Pacific cod during the Pleistocene.
BIOSCIENCE REPORTS
(2022)
Article
Environmental Sciences
Brian R. C. Kennedy, Lara Hakam, Jan Witting, Regen Milani, Sue Taei, Tim Smith, Erin Taylor, Tooreka Teemari, Randi D. Rotjan
Summary: The study reconstructed the distribution of sperm whales in and around the Phoenix Islands, finding that they were present year-round. Hotspot analysis indicated that PIPA and near Howland and Baker are sighting hotspots for sperm whales, suggesting long-term ecological importance in the central Pacific.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Immunology
Cameron L. Gardner, Mara Pavel-Dinu, Kerry Dobbs, Marita Bosticardo, Paul K. Reardon, Justin Lack, Suk See DeRavin, Kent Le, Ezekiel Bello, Francesca Pala, Ottavia M. Delmonte, Harry Malech, Amelie Montel-Hagan, Gay Crooks, Oreste Acuto, Matthew H. Porteus, Luigi D. Notarangelo
Summary: SCID caused by RAG1 or RAG2 deficiency is a genetically determined immune deficiency characterized by the virtual absence of T and B lymphocytes. Gene editing has shown promise in restoring normal function in patient cells, offering a novel therapeutic option for this immunodeficiency.
JOURNAL OF CLINICAL IMMUNOLOGY
(2021)
Article
Mathematics
Yi Xie
Summary: This paper introduces the annular instanton Floer homology and constructs a spectral sequence for links in a thickened annulus, with applications in detecting the unlink and distinguishing braids from other tangles using the annular Khovanov homology.
ADVANCES IN MATHEMATICS
(2021)
Article
Cell Biology
Yang Dong, Hao Guo, Donghai Wang, Rongfu Tu, Guoliang Qing, Hudan Liu
Summary: The study reveals that Notch1 transcriptional complexes directly activate Rag1 and Rag2 genes, with a novel cis-element SPS playing an important role in T-ALL and DN cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Vladimir A. Lukhtanov, Alexander Dantchenkou
Summary: This study used chromosomal and DNA-barcode markers to investigate the taxonomic relationships within the Polyommatus (Agrodiaetus) damone (Eversmann, 1841) species complex, revealing a new subspecies and six diverged clusters based on mitochondrial gene COI analysis with distinct morphological features. The findings suggest chromosomal stasis in this complex and provide insights into the genetic and morphological diversity within the group.
COMPARATIVE CYTOGENETICS
(2021)
Article
Chemistry, Multidisciplinary
Hiroshi Izumi, Hiroshi Aoki, Laurence A. Nafie, Rina K. Dukor
Summary: The Omicron BA.1 variant infects mainly through the cathepsin-mediated endocytic pathway, but the exact mechanism of cell entry is still unknown. Compared to BA.2, BA.4/5 is more fusogenic and spreads more efficiently in human lung cells. It remains unclear why the Omicron spike is not efficiently cleaved in virions compared to the Delta variant, and how it successfully reproduces without cell entry through plasma membrane fusion.
Article
Multidisciplinary Sciences
Daniel Allen, Orli Knop, Bryan Itkowitz, Nechama Kalter, Michael Rosenberg, Ortal Iancu, Katia Beider, Yu Nee Lee, Arnon Nagler, Raz Somech, Ayal Hendel
Summary: RAG2-SCID is an immunodeficiency disease, and the current treatment is allogeneic hematopoietic stem cell transplantation. Using CRISPR-Cas9/rAAV6 gene editing technology, we have demonstrated an innovative RAG2 correction strategy that replaces the patient's own hematopoietic stem and progenitor cells, offering an alternative therapy.
NATURE COMMUNICATIONS
(2023)
Article
Mathematics
Ben Knudsen, Jeremy Miller, Philip Tosteson
Summary: We study the stability patterns in the high dimensional rational homology of unordered configuration spaces of manifolds by applying a general approach to stability phenomena in the homology of Lie algebras.
MATHEMATISCHE ANNALEN
(2023)