Optimization of Peripheral Blood Mononuclear Cell Processing for Improved Clinical ELISpot Assay Performance

Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes

(2023) Bryan Linggi et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosis

(2022) Tobias Burkard et al.   Clinical and Translational Medicine

Early Phase Clinical Immunogenicity of Valoctocogene Roxaparvovec, an AAV5-Mediated Gene Therapy for Hemophilia A

(2020) Brian R. Long et al.   MOLECULAR THERAPY

Prevalence of Anti-Adeno-Associated Virus Immune Responses in International Cohorts of Healthy Donors

(2019) Anita Kruzik et al.   Molecular Therapy-Methods & Clinical Development

Improved Expansion and In Vivo Function of Patient T Cells by a Serum-free Medium

(2018) Andrew R. Medvec et al.   Molecular Therapy-Methods & Clinical Development

Hemophilia B Gene Therapy with a High-Specific-Activity Factor IX Variant

(2017) Lindsey A. George et al.   NEW ENGLAND JOURNAL OF MEDICINE

High-density preculture of PBMCs restores defective sensitivity of circulating CD8 T cells to virus- and tumor-derived antigens

(2015) J. Wegner et al.   BLOOD

Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis

(2014) Radleigh Santos et al.   Cells

Overnight Resting of PBMC Changes Functional Signatures of Antigen Specific T- Cell Responses: Impact for Immune Monitoring within Clinical Trials

(2013) Sarah Kutscher et al.   PLoS One

Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols

(2012) Helene Filbert et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Humoral and Cellular Capsid-Specific Immune Responses to Adeno-Associated Virus Type 1 in Randomized Healthy Donors

(2012) P. Veron et al.   JOURNAL OF IMMUNOLOGY

Preculture of PBMCs at high cell density increases sensitivity of T-cell responses, revealing cytokine release by CD28 superagonist TGN1412

(2011) P. S. Romer et al.   BLOOD

Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function

(2011) Walter C Olson et al.   Journal of Translational Medicine

Critical parameters in blood processing for T-cell assays: Validation on ELISpot and tetramer platforms

(2010) Georgia Afonso et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Performance of serum-supplemented and serum-free media in IFNγ Elispot Assays for human T cells

(2009) Sylvia Janetzki et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Delayed processing of blood increases the frequency of activated CD11b+ CD15+ granulocytes which inhibit T cell function

(2008) Kyle C. McKenna et al.   JOURNAL OF IMMUNOLOGICAL METHODS