Article
Immunology
Zihan Li, Qiuxia Huang, Zhuoran Wang, Lijia Huang, Lisha Gu
Summary: Periodontitis is a dysbiosis condition caused by the coordinated action of multiple members within the microbial consortium. This study demonstrates that Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can alter the structure and composition of subgingival microbiome, leading to dysbiosis and impaired epithelium function. P. gingivalis drives the commensal microbiome towards dysbiosis, while A. actinomycetemcomitans enhances the symbiotic state.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Microbiology
Rolf Claesson, Anders Johansson, Georgios N. Belibasakis
Summary: The aim of this study was to compare the prevalence and proportions of three bacterial species in periodontitis pocket samples from young and old patients. The results showed that the prevalence of Aggregatibacter actinomycetemcomitans was higher in young patients, while Porphyromonas gingivalis was similarly distributed in both age groups. Parvimonas micra was more prevalent in old patients. Furthermore, the proportion of samples containing A. actinomycetemcomitans and P. micra together was higher than when P. gingivalis was present.
Article
Biotechnology & Applied Microbiology
Rui Li, Mengjie Hou, Liying Yu, Wen Luo, Jie Kong, Renmei Yu, Ruihan Liu, Qian Li, Lisi Tan, Chunling Pan, Hongyan Wang
Summary: This study aimed to investigate the effects of salivary histatin 5 (Hst5) on Porphyromonas gingivalis (P. gingivalis) biofilms in vitro and in vivo and the possible mechanisms. The results showed that 25μg/mL Hst5 effectively inhibited biofilm formation and increased concentrations of Hst5 increased the inhibitive effect. Hst5 could regulate membrane function and metabolic processes in P. gingivalis, and RpoD and FeoB proteins might play important roles in this process. Moreover, 100μg/mL Hst5 inhibited periodontal inflammation and alveolar bone loss in rat periodontitis through its antibacterial and anti-inflammatory effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Thomas E. Rams, Adolfo Contreras, Jorgen Slots
Summary: This study found a high prevalence of early-age aggressive periodontitis in Pueblo Indian adolescents, mainly influenced by periodontopathic bacteria and herpesviruses.
JOURNAL OF PERIODONTOLOGY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Chaitanya Joshi, Ranjeet Bapat, William Anderson, Dana Dawson, George Cherukara, Karolin Hijazi
Summary: The meta-analysis showed a significant association between elevated serum IgG antibody responses (anti-Pg and anti-Aa) and CHD, suggesting their potential use as biomarkers for detecting and monitoring at-risk populations. However, inconsistencies in the design and interpretation of immunoassays highlight the need for standardization in assessing antibody responses against periodontal bacteria.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
Nabil Khzam, Leticia Algarves Miranda, Omar Kujan, Kate Shearston, Dorte Haubek
Summary: This study investigated the global prevalence of the JP2 genotype of Aggregatibacter actinomycetemcomitans (Aa). The results showed a relatively high presence of the JP2 genotype in subjects from South America, North America, and Africa. However, there were no studies estimating the presence of the JP2 genotype in the Oceania region. Due to the heterogeneity and quality of the included publications, caution should be exercised when interpreting the data, and there is a need for additional evidence.
CLINICAL ORAL INVESTIGATIONS
(2022)
Review
Pharmacology & Pharmacy
Thanyaporn Sang-Ngoen, Laszlo Mark Czumbel, Wuttapon Sadaeng, Alexandra Miko, David Istvan Nemeth, Peter Matrai, Peter Hegyi, Barbara Toth, Dezso Csupor, Istvan Kiss, Andrea Szabo, Gabor Gerber, Gabor Varga, Beata Keremi
Summary: The study found that probiotics have a beneficial effect in reducing the count of A. actinomycetemcomitans, but do not have a significant impact on other key periodontal pathogenic bacteria. Further well-designed randomized clinical trials are needed to assess the efficacy of probiotics in managing periodontal disease.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Gina R. Lewin, Kendall S. Stocke, Richard J. Lamont, Marvin Whiteley
Summary: This study characterizes the behavior and virulence of the periodontal pathogen Porphyromonas gingivalis during human infection. It reveals that P. gingivalis is highly expressed in diseased samples, with genes related to virulence factors and growth/matabolism being actively expressed. The study also shows that the gene expression of P. gingivalis during periodontitis is highly conserved with logarithmic laboratory growth, suggesting that simple test tube growth can be used as a model for studying P. gingivalis biology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cardiac & Cardiovascular Systems
Chaitanya Joshi, Ranjeet Bapat, William Anderson, Dana Dawson, Karolin Hijazi, George Cherukara
Summary: This systematic review found a high prevalence of periodontal bacterial DNA in coronary atherosclerotic plaque specimens, with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans being the most commonly detected species. In addition to periodontal microbes, DNA from a variety of other microbes was also recovered from the collected samples.
TRENDS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Chemistry, Analytical
Seonhwa Park, Kiryeon Park, Hee Sam Na, Jin Chung, Haesik Yang
Summary: An electrochemical biosensor for sensitive detection of Porphyromonas gingivalis in saliva was developed without the need for washing and separation steps. This biosensor can be used as an initial diagnosis method to distinguish between healthy people and patients with periodontal diseases.
ANALYTICAL CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Fahad A. Alshehri, Meshal S. Alharbi
Summary: This systematic review evaluated the effectiveness of hyaluronic acid (HA) as an adjunctive topical antibacterial agent in reducing the prevalence of P. gingivalis in subgingival biofilms during non-surgical mechanical therapy of periodontitis. Five clinical studies were included in the analysis, and only three studies provided data for meta-analysis at three and six months. The results did not support the use of HA to reduce the prevalence of P. gingivalis in subgingival biofilm.
Article
Immunology
Banndith Cheat, Coralie Torrens, Asmaa Foda, Brigitte Baroukh, Jeremy Sadoine, Lotfi Slimani, Veronique Witko-Sarsat, Olivier Huck, Marjolaine Gosset, Jerome Bouchet
Summary: Our study investigates the role of NLRP3 in periodontitis, demonstrating its ambiguous role in neutrophils and its interaction with Porphyromonas gingivalis. The study reveals that NLRP3 is involved in regulatory pathways that limit periodontitis in mouse models, but its protective effect is diminished in the presence of P. gingivalis. Overexpression of NLRP3 in connective tissue leads to increased production of mature IL-1 beta and mobilization of neutrophils, while NLRP3 deficiency results in lower IL-1 beta expression and almost no neutrophil mobilization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Evelin Witkowska, Anna M. Lasica, Krzysztof Nicinski, Jan Potempa, Agnieszka Kaminska
Summary: Recent studies suggest a potential link between Porphyromonas gingivalis and Alzheimer's disease. A method using surface-enhanced Raman scattering (SERS) to easily identify P. gingivalis and distinguish it from other pathogens has been proposed. By utilizing magnetic nanoparticles and microfluidic chips, bacteria can be separated for further analysis.
Article
Multidisciplinary Sciences
Min Wang, Lili Li, Jun Qian, Nannan Wang, Jun Bao, Jiangyue Lu, Faming Chen, Yanfen Li, Yangheng Zhang, Fuhua Yan
Summary: In this study, the salivary microbiota from patients with periodontitis was transferred to obese mice induced by high-fat diet to investigate the influence on gut microbiome and liver metabolism. The results showed that periodontitis salivary microbiota altered gut microbiota composition and exacerbated intestinal barrier dysfunction, leading to activation of toll-like receptor 4 signaling and release of pro-inflammatory factors in the liver. Moreover, the upregulation of the tryptophan-kynurenine-AhR signal axis in the liver was associated with aggravated hepatic steatosis and dysregulation of glucolipid metabolism during NAFLD development. This study suggests that periodontitis salivary microbiota may worsen the pathological progression of NAFLD in the context of obesity, with the tryptophan-AhR pathway potentially playing a key role.
Article
Immunology
Xiaomiao Fan, Shaowen Zheng, Chen Chen, Li Lin, Hongyan Wang, Yuqin Shen, Yaping Pan, Chen Li
Summary: This study investigated the role of sialidase in immune evasion of P. gingivalis by examining its effects on macrophage polarization, antigen presentation, and phagocytosis. The results showed that Delta PG0352 strain, lacking the sialidase gene, was more easily cleared by macrophages and exhibited increased M1 polarization, antigen presentation, and phagocytosis compared to the wild-type strain. This suggests that sialidase plays an important role in P. gingivalis immune evasion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
