Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 46, Pages 9487-9490Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc02817e
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Funding
- National Natural Science Funds of China [21375102, 21005061]
- Natural Science Basic Research Plan in Shaanxi Province of China [2012KJXX-25]
- Science and Technology Plan Project of Xi'an [CXY1441(8)]
- Open Foundation of Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education [338080059]
- Education Department of Shaanxi Province, China [12JS088]
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An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2'-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransferase over a dynamic concentration range (0.1 U mL(-1)-100 U mL(-1)) with an extremely low detection limit of 0.03 U mL(-1), using gold nanoparticles and a quenching ECL signal produced by a chemical quencher such as ferrocene.
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