Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 54, Pages 10945-10948Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cc02409a
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Funding
- KNRF grant - Korean government [NRF-2013R1A1A1008228, NRF-2010-0028684]
- KRIBB Research Initiative Program
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A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
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